Strain identifier

BacDive ID: 8147

Type strain: Yes

Species: Acinetobacter rudis

Strain Designation: G30

Strain history: CIP <- 2011, CCUG <- 2009, C.M. Manaia, Catolica Portuguesa Univ., Porto, Portugal <- E. Hantsis Zacharov <- M. Halpern, Haifa Univ., Oranim, Tivon, Israel: strain G30

NCBI tax ID(s): 632955 (species)

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General

@ref: 17480

BacDive-ID: 8147

DSM-Number: 24031

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Acinetobacter rudis G30 is an aerobe, mesophilic bacterium that was isolated from raw milk.

NCBI tax id

  • NCBI tax id: 632955
  • Matching level: species

strain history

@refhistory
17480<- C. M. Manaia, Escola Superior Biotecnol. (UCP), Porto, Portugal; G30 <- E. Hantsis-Zacharov and M. Halpern, Univ. Haifa, Oranim, Tivon, Israel
122300CIP <- 2011, CCUG <- 2009, C.M. Manaia, Catolica Portuguesa Univ., Porto, Portugal <- E. Hantsis Zacharov <- M. Halpern, Haifa Univ., Oranim, Tivon, Israel: strain G30

doi: 10.13145/bacdive8147.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter rudis
  • full scientific name: Acinetobacter rudis Vaz-Moreira et al. 2011

@ref: 17480

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter rudis

full scientific name: Acinetobacter rudis Vaz-Moreira et al. 2011

strain designation: G30

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.766
6948099.992negative
122300nonegativeoval-shaped

colony morphology

  • @ref: 17480
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17480R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
42129MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122300CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17480positivegrowth30mesophilic
42129positivegrowth30mesophilic
61511positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
61511aerobe
122300obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
12230017632nitrate-reduction
12230016301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
12230035581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
17480catalase+1.11.1.6
17480cytochrome-c oxidase-1.9.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
122300oxidase-
122300catalase+1.11.1.6
122300urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122300-+++-++---++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
17480++-------------+-+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17480raw milknorthern IsraelIsraelISRAsia
61511Raw milkNorthern IsraelIsraelISRAsia
122300Food, Raw milkHaifaIsraelISRAsia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Host#Mammals

taxonmaps

  • @ref: 69479
  • File name: preview.99_2111.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_1159;97_1362;98_1651;99_2111&stattab=map
  • Last taxonomy: Acinetobacter rudis subclade
  • 16S sequence: EF204258
  • Sequence Identity:
  • Total samples: 2700
  • soil counts: 975
  • aquatic counts: 429
  • animal counts: 1186
  • plant counts: 110

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
174801Risk group (German classification)
1223001Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17480
  • description: Acinetobacter rudis CIP 110305 strain G30 16S ribosomal RNA gene, partial sequence
  • accession: EF204258
  • length: 1500
  • database: ena
  • NCBI tax ID: 421052

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter rudis DSM 24031GCA_000829675contigncbi632955
66792Acinetobacter rudis strain DSM 24031632955.6wgspatric632955
66792Acinetobacter rudis DSM 240312671181009draftimg632955

GC content

  • @ref: 17480
  • GC-content: 39.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno97.365no
anaerobicno96.996no
halophileno87.021no
spore-formingno96.71no
glucose-utilno88.723yes
thermophileno99.817no
flagellatedno94.378no
aerobicyes88.076no
motileno88.489no
glucose-fermentno90.294yes

External links

@ref: 17480

culture collection no.: DSM 24031, CCUG 57889, CECT 7818, LMG 26107, CIP 110305

straininfo link

  • @ref: 77550
  • straininfo: 371127

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21239566Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater.Vaz-Moreira I, Novo A, Hantsis-Zacharov E, Lopes AR, Gomila M, Nunes OC, Manaia CM, Halpern MInt J Syst Evol Microbiol10.1099/ijs.0.027045-02011Acinetobacter/*classification/genetics/*isolation & purification/metabolism, Animals, Base Composition, Cattle, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Milk/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiologyMetabolism
Phylogeny27902257Acinetobacter larvae sp. nov., isolated from the larval gut of Omphisa fuscidentalis.Liu S, Wang Y, Ruan Z, Ma K, Wu B, Xu Y, Wang J, You Y, He M, Hu GInt J Syst Evol Microbiol10.1099/ijsem.0.0016442017Acinetobacter/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Larva/microbiology, Moths/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
17480Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24031)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24031
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42129Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8150
61511Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57889)https://www.ccug.se/strain?id=57889
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77550Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID371127.1StrainInfo: A central database for resolving microbial strain identifiers
122300Curators of the CIPCollection of Institut Pasteur (CIP 110305)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110305