Strain identifier

BacDive ID: 8140

Type strain: Yes

Species: Acinetobacter brisouii

Strain Designation: 5YN5-8

Strain history: CIP <- 2011, DSMZ <- S.W. Kwon, KACC, Korea: strain 5YN5-8

NCBI tax ID(s): 1341683 (strain), 396323 (species)

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General

@ref: 7569

BacDive-ID: 8140

DSM-Number: 18516

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Acinetobacter brisouii 5YN5-8 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from peat layer of a high moor.

NCBI tax id

NCBI tax idMatching level
1341683strain
396323species

strain history

@refhistory
7569<- S.-W. Kwon, KACC; 5YN5-8
117047CIP <- 2011, DSMZ <- S.W. Kwon, KACC, Korea: strain 5YN5-8

doi: 10.13145/bacdive8140.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter brisouii
  • full scientific name: Acinetobacter brisouii Anandham et al. 2011

@ref: 7569

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter brisouii

full scientific name: Acinetobacter brisouii Anandham et al. 2011

strain designation: 5YN5-8

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.991
117047negativeoval-shapedno

colony morphology

@refincubation period
75691-2 days
626781 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7569R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
37440MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
117047CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
7569positivegrowth28mesophilic
37440positivegrowth30mesophilic
62678positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
62678aerobe
117047obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11704717632nitrate-reduction
11704716301nitrite-reduction

metabolite production

  • @ref: 117047
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
117047oxidase-
117047catalase-1.11.1.6
117047urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117047-+++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
7569peat layer of a high moorYongneup wetland, 1200 m above sea level (38° 12' 53'' N 128° 07' 30'' E)Republic of KoreaKORAsia38.2147128.125
62678Peat layer,high moorYongneup wetland,1200m above sea lRepublic of KoreaKORAsia
117047Peat layer of a high moorYongneup WetlandRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Wetland (Swamp)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
75691Risk group (German classification)
1170471Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7569
  • description: Acinetobacter brisouii strain 5YN5-8 16S ribosomal RNA gene, partial sequence
  • accession: DQ832256
  • length: 1405
  • database: ena
  • NCBI tax ID: 396323

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter brisouii CIP 110357GCA_000488275scaffoldncbi1341683
66792Acinetobacter brisouii CIP 110357 DSM 18516GCA_000931655contigncbi1341683
66792Acinetobacter brisouii CIP 1103571341683.3wgspatric1341683
66792Acinetobacter brisouii CIP 110357 strain DSM 185161341683.5wgspatric1341683
66792Acinetobacter brisouii CIP 1103572657245517draftimg1341683
66792Acinetobacter brisouii CIP 1103572579778708draftimg1341683

GC content

  • @ref: 7569
  • GC-content: 43.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno89.683no
flagellatedno91.32no
gram-positiveno98.061no
anaerobicno97.579yes
aerobicyes85.518yes
halophileno90.122no
spore-formingno97.374no
thermophileno99.799no
glucose-utilno79.167no
glucose-fermentno89.948no

External links

@ref: 7569

culture collection no.: DSM 18516, CIP 110357, KACC 11602, CCUG 61636, CIP 110501, ANC 4119

straininfo link

  • @ref: 77544
  • straininfo: 377740

literature

  • topic: Phylogeny
  • Pubmed-ID: 20221727
  • title: Acinetobacter brisouii sp. nov., isolated from a wetland in Korea.
  • authors: Anandham R, Weon HY, Kim SJ, Kim YS, Kim BY, Kwon SW
  • journal: J Microbiol
  • DOI: 10.1007/s12275-009-0132-8
  • year: 2010
  • mesh: Acinetobacter/chemistry/*classification/genetics/*isolation & purification/metabolism, Fatty Acids, Genes, Bacterial, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Soil Microbiology, *Wetlands
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7569Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18516)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18516
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37440Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8207
62678Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61636)https://www.ccug.se/strain?id=61636
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77544Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID377740.1StrainInfo: A central database for resolving microbial strain identifiers
117047Curators of the CIPCollection of Institut Pasteur (CIP 110357)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110357