Strain identifier

BacDive ID: 8132

Type strain: Yes

Species: Acinetobacter gerneri

Strain Designation: 9AO1, 9A01

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 9A01

NCBI tax ID(s): 1120926 (strain), 202952 (species)

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General

@ref: 5642

BacDive-ID: 8132

DSM-Number: 14967

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, oval-shaped

description: Acinetobacter gerneri 9AO1 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Wastewater treatment plant.

NCBI tax id

NCBI tax idMatching level
1120926strain
202952species

strain history

@refhistory
5642<- P. Kämpfer; 9AO1 <- G. Knight
421512002, P. Kämpfer, Univ., Giessen, Germany: strain 9A01
119608CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 9A01

doi: 10.13145/bacdive8132.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter gerneri
  • full scientific name: Acinetobacter gerneri Carr et al. 2003

@ref: 5642

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter gerneri

full scientific name: Acinetobacter gerneri Carr et al. 2003

strain designation: 9AO1, 9A01

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
119608negativeoval-shapedno
125438negative99.5

colony morphology

  • @ref: 119608

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_14967_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14967_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14967_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14967_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_14967_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5642CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5642TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42151MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119608CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
5642positivegrowth30
42151positivegrowth30
119608positivegrowth10-41
119608nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
119608obligate aerobe
125439facultative anaerobe98.1

spore formation

@refspore formationconfidence
125438no96.248
125439no98.6

halophily

@refsaltgrowthtested relationconcentration
119608NaClpositivegrowth0-2 %
119608NaClnogrowth4 %
119608NaClnogrowth6 %
119608NaClnogrowth8 %
119608NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11960817632nitrate-reduction
11960816301nitrite-reduction
11960817632nitrate-respiration
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

antibiotic resistance

  • @ref: 119608
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11960835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11960815688acetoin-
11960817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119608oxidase-
119608beta-galactosidase-3.2.1.23
119608gelatinase-
119608catalase+1.11.1.6
119608gamma-glutamyltransferase+2.3.2.2
119608urease-3.5.1.5
68369gelatinase-
68369urease-3.5.1.5
68382alpha-galactosidase-3.2.1.22
68369cytochrome oxidase-1.9.3.1
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase-3.5.3.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119608--++++----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5642---------------+++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119608-------------------------------------------------+---+----+--+++-+-+++---+-+++-+++---+--+-++++--++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5642Wastewater treatment plantVictoriaAustraliaAUSAustralia and Oceania
119608Environment, Bendigo waste water treatment plantVictoriaAustraliaAUSAustralia and Oceania1991

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Engineered#Waste#Water treatment plant

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56421Risk group (German classification)
1196081Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter gerneri strain DSM14967 16S ribosomal RNA gene, partial sequenceEF611410453nuccore202952
20218Acinetobacter gerneri genomic DNA containing 16S-23S IGS, strain DSM 14967, direct sequence AllHE651864659nuccore202952
20218Acinetobacter gerneri 16S rRNA gene, strain DSM 14967HE6519261530nuccore202952
5642Acinetobacter gerneri 16S ribosomal RNA gene, partial sequenceAF5098291325nuccore202952
124043Acinetobacter gerneri DSM 14967 = CIP 107464 = MTCC 9824 16S ribosomal RNA gene, partial sequence.OP8181001366nuccore1120926

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter gerneri DSM 14967 = CIP 107464 = MTCC 9824GCA_000368565scaffoldncbi1120926
66792Acinetobacter gerneri DSM 14967 = CIP 107464 = MTCC 9824GCA_000430245contigncbi1120926
66792Acinetobacter gerneri DSM 14967 = CIP 1074641120926.3wgspatric1120926
66792Acinetobacter gerneri DSM 149672565956694draftimg1120926

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno99.5no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.349yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.202no
125438spore-formingspore-formingAbility to form endo- or exosporesno96.248no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno98.5yes
125438motile2+flagellatedAbility to perform flagellated movementno74.245no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.6
125439BacteriaNetmotilityAbility to perform movementno66.8
125439BacteriaNetgram_stainReaction to gram-stainingpositive75.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthfacultative anaerobe98.1

External links

@ref: 5642

culture collection no.: DSM 14967, CIP 107464, CCM 7197

straininfo link

  • @ref: 77538
  • straininfo: 113568

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12892111Seven novel species of Acinetobacter isolated from activated sludge.Carr EL, Kampfer P, Patel BKC, Gurtler V, Seviour RJInt J Syst Evol Microbiol10.1099/ijs.0.02486-02003Acinetobacter/*classification/genetics/*isolation & purification, Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Terminology as Topic, VictoriaGenetics
Phylogeny33591460Acinetobacter stercoris sp. nov. isolated from output source of a mesophilic german biogas plant with anaerobic operating conditions.Pulami D, Schauss T, Eisenberg T, Blom J, Schwengers O, Bender JK, Wilharm G, Kampfer P, Glaeser SPAntonie Van Leeuwenhoek10.1007/s10482-021-01517-72021*Acinetobacter/genetics, Anaerobiosis, Bacterial Typing Techniques, Base Composition, *Biofuels, DNA, Bacterial/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5642Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14967)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14967
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42151Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4902
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
77538Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113568.1StrainInfo: A central database for resolving microbial strain identifiers
119608Curators of the CIPCollection of Institut Pasteur (CIP 107464)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107464
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1