Strain identifier

BacDive ID: 8131

Type strain: Yes

Species: Acinetobacter bouvetii

Strain Designation: 4BO2, 4B02

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 4B02

NCBI tax ID(s): 1120925 (strain), 202951 (species)

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General

@ref: 5639

BacDive-ID: 8131

DSM-Number: 14964

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Acinetobacter bouvetii 4BO2 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Wastewater treatment plant.

NCBI tax id

NCBI tax idMatching level
202951species
1120925strain

strain history

@refhistory
5639<- P. Kämpfer; 4BO2 <- G. Knight
421492002, P. Kämpfer, Univ., Giessen, Germany: strain 4B02
67770DSM 14964 <-- P. Kämpfer 4B02 <-- G. Knight.
116903CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 4B02

doi: 10.13145/bacdive8131.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter bouvetii
  • full scientific name: Acinetobacter bouvetii Carr et al. 2003

@ref: 5639

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter bouvetii

full scientific name: Acinetobacter bouvetii Carr et al. 2003

strain designation: 4BO2, 4B02

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.986
116903negativeoval-shapedno

colony morphology

  • @ref: 116903

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5639CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5639TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42149MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116903CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5639positivegrowth30mesophilic
42149positivegrowth30mesophilic
58979positivegrowth30mesophilic
67770positivegrowth30mesophilic
116903positivegrowth10-41
116903nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58979aerobe
116903obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

halophily

@refsaltgrowthtested relationconcentration
116903NaClpositivegrowth0-2 %
116903NaClnogrowth4 %
116903NaClnogrowth6 %
116903NaClnogrowth8 %
116903NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11690317632nitrate-reduction
11690316301nitrite-reduction
11690317632nitrate-respiration

antibiotic resistance

  • @ref: 116903
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11690335581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11690315688acetoin-
11690317234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
116903oxidase-
116903beta-galactosidase+3.2.1.23
116903gelatinase-
116903catalase+1.11.1.6
116903gamma-glutamyltransferase-2.3.2.2
116903urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116903--++-+--------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5639---------------+-+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116903-------------------------------------------------+---+--------+--+-+++-----+++++++----+---++++--+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5639Wastewater treatment plantVictoriaAustraliaAUSAustralia and Oceania
58979Activated sludge
67770Activated sludge plants in VictoriaAustraliaAUSAustralia and Oceania
116903Environment, Bendigo waste water treatment plantVictoriaAustraliaAUSAustralia and Oceania1991

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Engineered#Waste#Water treatment plant

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56391Risk group (German classification)
1169031Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter bouvetii strain DSM14964 16S ribosomal RNA gene, partial sequenceEF611408452ena202951
20218Acinetobacter bouvetii genomic DNA containing 16S-23S IGS, strain DSM 14964, direct sequence AllHE651863668ena202951
20218Acinetobacter bouvetii 16S rRNA gene, strain DSM 14964HE6519271530ena202951
5639Acinetobacter bouvetii 16S ribosomal RNA gene, partial sequenceAF5098271325ena202951

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter bouvetii JCM 18991GCA_018324105completencbi202951
66792Acinetobacter bouvetii DSM 14964 = CIP 107468 [PRJNA199256]1120925.4wgspatric1120925
66792Acinetobacter bouvetii DSM 14964 = CIP 107468 [PRJNA201594]1120925.3wgspatric1120925
66792Acinetobacter bouvetii strain GTC 3697202951.3wgspatric202951
66792Acinetobacter bouvetii strain JCM 18991202951.7completepatric202951
66792Acinetobacter bouvetii DSM 149642565956682draftimg1120925
66792Acinetobacter bouvetii GTC 36972660238193draftimg202951
66792Acinetobacter bouvetii DSM 149642517093005draftimg1120925
67770Acinetobacter bouvetii DSM 14964 = CIP 107468GCA_000368865scaffoldncbi1120925
67770Acinetobacter bouvetii DSM 14964 = CIP 107468GCA_000373725scaffoldncbi1120925
67770Acinetobacter bouvetii GTC 3697GCA_001485025contigncbi202951

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno95.831no
gram-positiveno97.831no
anaerobicno97.59yes
aerobicyes90.265no
halophileno89.696no
spore-formingno97.755no
thermophileno99.886no
glucose-utilno91.638yes
motileno92.384no
glucose-fermentno90.847yes

External links

@ref: 5639

culture collection no.: CCUG 50766, DSM 14964, CIP 107468, JCM 18991, CCM 7196, GTC 3697, KCTC 12414, NBRC 112580

straininfo link

  • @ref: 77537
  • straininfo: 113565

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12892111Seven novel species of Acinetobacter isolated from activated sludge.Carr EL, Kampfer P, Patel BKC, Gurtler V, Seviour RJInt J Syst Evol Microbiol10.1099/ijs.0.02486-02003Acinetobacter/*classification/genetics/*isolation & purification, Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Terminology as Topic, VictoriaGenetics
Metabolism32926028Precursor-directed biosynthesis of catechol compounds in Acinetobacter bouvetii DSM 14964.Reitz ZL, Butler AChem Commun (Camb)10.1039/d0cc04171h2020Acinetobacter/*chemistry/metabolism, Catechols/chemistry/*metabolism, Molecular Structure, Stereoisomerism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5639Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14964)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14964
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42149Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4907
58979Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50766)https://www.ccug.se/strain?id=50766
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77537Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113565.1StrainInfo: A central database for resolving microbial strain identifiers
116903Curators of the CIPCollection of Institut Pasteur (CIP 107468)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107468