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Strain identifier

BacDive ID: 81

Type strain: Yes

Species: Acidisoma tundrae

Strain Designation: WM1

Strain history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; WM1 <- S. Belova

NCBI tax ID(s): 512382 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8416

BacDive-ID: 81

DSM-Number: 19999

keywords: 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, psychrophilic, Gram-negative, coccus-shaped, colony-forming

description: Acidisoma tundrae WM1 is an obligate aerobe, chemoorganotroph, psychrophilic bacterium that forms circular colonies and was isolated from acidic oligotrophic Sphagnum-dominated wetland.

NCBI tax id

  • NCBI tax id: 512382
  • Matching level: species

strain history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; WM1 <- S. Belova

doi: 10.13145/bacdive81.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acidisoma
  • species: Acidisoma tundrae
  • full scientific name: Acidisoma tundrae Belova et al. 2009

@ref: 8416

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acidisoma

species: Acidisoma tundrae

full scientific name: Acidisoma tundrae Belova et al. 2009

strain designation: WM1

type strain: yes

Morphology

cell morphology

  • @ref: 22964
  • gram stain: negative
  • cell length: 1.9-4.1 µm
  • cell width: 0.8-1.5 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 22964
  • colony size: 2.0-5.0 mm
  • colony color: white or ivory
  • colony shape: circular
  • incubation period: 14 days

Culture and growth conditions

culture medium

  • @ref: 8416
  • name: ACIDOSOMA MEDIUM (DSMZ Medium 1220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1220
  • composition: Name: ACIDOSOMA MEDIUM (DSMZ Medium 1220) Composition: Agar 15.0 g/l Na gluconate 0.5 g/l (NH4)2SO4 0.25 g/l KH2PO4 0.1 g/l Yeast extract 0.1 g/l CaCl2 x 2 H2O 0.05 g/l MgSO4 x 7 H2O 0.02 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l CuCl2 x 5 H2O 0.0001 g/l Na2MoO4 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
8416positivegrowth20psychrophilic
22964positivegrowth2.0-30.0
22964positiveoptimum15.0-22.0psychrophilic

culture pH

@refabilitytypepHPH range
22964positivegrowth3.0-7.5acidophile
22964positiveoptimum4.5-5.7

Physiology and metabolism

oxygen tolerance

  • @ref: 22964
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 22964
  • type: chemoorganotroph

spore formation

  • @ref: 22964
  • spore formation: no

halophily

  • @ref: 22964
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: >1.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2296430089acetate-growth
2296416449alanine-growth
2296435391aspartate-growth
2296416150benzoate-growth
2296428885butanol-growth
2296417057cellobiose-growth
2296416947citrate-growth
2296415748D-glucuronate-growth
2296415740formate-growth
2296429806fumarate-growth
2296417120hexanoate-growth
2296417716lactose-growth
2296425017leucine-growth
2296417306maltose-growth
2296417790methanol-growth
2296430623oxalate-growth
2296417272propionate-growth
2296416634raffinose-growth
2296430031succinate-growth
2296417992sucrose-growth
2296458187alginate-hydrolysis
2296485146carboxymethylcellulose-hydrolysis
2296437397chondroitin sulfate-hydrolysis
229646364laminarin-hydrolysis
2296427941pullulan-hydrolysis
2296417632nitrate-reduction
2296422599arabinose+carbon source
2296417968butyrate+carbon source
22964casamino acids+carbon source
2296418024D-galacturonic acid+carbon source
2296416813galactitol+carbon source
2296416236ethanol+carbon source
2296428757fructose+carbon source
2296428260galactose+carbon source
2296424265gluconate+carbon source
2296417234glucose+carbon source
2296417754glycerol+carbon source
2296417196L-asparagine+carbon source
2296424996lactate+carbon source
2296425115malate+carbon source
2296429864mannitol+carbon source
2296437684mannose+carbon source
2296417268myo-inositol+carbon source
22964peptone+carbon source
2296415361pyruvate+carbon source
2296430911sorbitol+carbon source
2296427922sorbose+carbon source
2296427082trehalose+carbon source
2296418222xylose+carbon source
22964yeast extract+carbon source
229644853esculin+hydrolysis
229645181fucoidan+hydrolysis
2296428304heparin+hydrolysis
2296417309pectin+hydrolysis
2296428017starch+hydrolysis
2296437166xylan+hydrolysis
2296416449alanine+nitrogen source
2296428938ammonium+nitrogen source
2296428300glutamine+nitrogen source
2296427570histidine+nitrogen source
2296416811methionine+nitrogen source
2296417632nitrate+nitrogen source
2296426271proline+nitrogen source
2296416199urea+nitrogen source
22964yeast extract+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2296428971ampicillinyesyes
229646472lincomycinyesyes
2296417698chloramphenicolyesyes
2296417833gentamicinyesyes
229646104kanamycinyesyes
229647507neomycinyesyes
2296428368novobiocinyesyes
2296417076streptomycinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2296430033bacteriochlorophyll alphano
229648295beta-hydroxybutyrateyes

enzymes

@refvalueactivityec
22964acid phosphatase+3.1.3.2
22964alkaline phosphatase-3.1.3.1
22964alpha-chymotrypsin-3.4.21.1
22964alpha-fucosidase-3.2.1.51
22964alpha-galactosidase-3.2.1.22
22964alpha-glucosidase+3.2.1.20
22964alpha-mannosidase-3.2.1.24
22964beta-galactosidase-3.2.1.23
22964beta-glucosidase-3.2.1.21
22964beta-glucuronidase-3.2.1.31
22964catalase+1.11.1.6
22964cystine arylamidase+3.4.11.3
22964cytochrome oxidase+1.9.3.1
22964esterase+
22964esterase lipase (C 8)+
22964leucine arylamidase+3.4.11.1
22964lipase (C 14)-
22964N-acetyl-beta-glucosaminidase-3.2.1.52
22964naphthol-AS-BI-phosphohydrolase-
22964phosphatidate phosphatase+3.1.3.4
22964trypsin-3.4.21.4
22964valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitude
8416acidic oligotrophic Sphagnum-dominated wetlandSphagnumVorkuta regionRussiaRUSAsia6763
22964wetlandVorkuta regionRussiaRUSAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Peat moss
#Condition#Acidic
#Environmental#Terrestrial#Wetland (Swamp)

taxonmaps

  • @ref: 69479
  • File name: preview.99_120020.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_24648;97_30866;98_75306;99_120020&stattab=map
  • Last taxonomy: Acidisoma tundrae subclade
  • 16S sequence: AM947652
  • Sequence Identity:
  • Total samples: 1053
  • soil counts: 566
  • aquatic counts: 184
  • animal counts: 125
  • plant counts: 178

Safety information

risk assessment

  • @ref: 8416
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8416
  • description: Acidisoma tundrae partial 16S rRNA gene, type strain WM1
  • accession: AM947652
  • length: 1400
  • database: ena
  • NCBI tax ID: 512382

GC content

  • @ref: 8416
  • GC-content: 61.9
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 8416

culture collection no.: DSM 19999, VKM B-2488

straininfo link

@refpassport
20218http://www.straininfo.net/strains/844395
20218http://www.straininfo.net/strains/844396

literature

  • topic: Phylogeny
  • Pubmed-ID: 19620354
  • title: Acidisoma tundrae gen. nov., sp. nov. and Acidisoma sibiricum sp. nov., two acidophilic, psychrotolerant members of the Alphaproteobacteria from acidic northern wetlands.
  • authors: Belova SE, Pankratov TA, Detkova EN, Kaparullina EN, Dedysh SN
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.009209-0
  • year: 2009
  • mesh: Acetobacteraceae/*classification/*isolation & purification, Aerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cold Temperature, Cytoplasmic Granules/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Hydrogen-Ion Concentration, Hydroxybutyrates/analysis, Microscopy, Electron, Transmission, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polyesters/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Siberia, *Wetlands
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8416Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19999)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19999
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22964Svetlana E. Belova,Timofei A. Pankratov,Ekaterina N. Detkova,Elena N. Kaparullina,Svetlana N. Dedysh10.1099/ijs.0.009209-0Acidisoma tundrae gen. nov., sp. nov. and Acidisoma sibiricum sp. nov., two acidophilic, psychrotolerant members of the Alphaproteobacteria from acidic northern wetlandsIJSEM 59: 2283-2290 200919620354
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta