Strain identifier

BacDive ID: 8092

Type strain: Yes

Species: Acinetobacter haemolyticus

Strain Designation: 2446/60

Strain history: CIP <- 1964, W. Mannheim, Hyg. Inst. Marburg, Germany: strain 2446/60, "Achromobacter haemolyticus subsp. haemolyticus"

NCBI tax ID(s): 1217659 (strain), 29430 (species)

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General

@ref: 2923

BacDive-ID: 8092

DSM-Number: 6962

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Acinetobacter haemolyticus 2446/60 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from human sputum.

NCBI tax id

NCBI tax idMatching level
29430species
1217659strain

strain history

@refhistory
2923<- ATCC <- NCTC <- W. Stenzel; 2446/60
421531964, W. Mannheim, Hyg. Inst. Marburg, Germany: strain 2446/60, "Achromobacter haemolyticus subsp. haemolyticus"
119767CIP <- 1964, W. Mannheim, Hyg. Inst. Marburg, Germany: strain 2446/60, "Achromobacter haemolyticus subsp. haemolyticus"

doi: 10.13145/bacdive8092.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter haemolyticus
  • full scientific name: Acinetobacter haemolyticus (ex Stenzel and Mannheim 1963) Bouvet and Grimont 1986

@ref: 2923

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter haemolyticus

full scientific name: Acinetobacter haemolyticus (ex Stenzel and Mannheim 1963) Bouvet and Grimont 1986

strain designation: 2446/60

type strain: yes

Morphology

cell morphology

  • @ref: 119767
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis ability
2923beta1
1197671

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42153MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
2923TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
2923COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
119767CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
2923positivegrowth28mesophilic
42153positivegrowth30mesophilic
119767positivegrowth30-37mesophilic
119767nogrowth10psychrophilic
119767nogrowth25mesophilic
119767nogrowth41thermophilic
119767nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119767
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119767NaClpositivegrowth0-4 %
119767NaClnogrowth6 %
119767NaClnogrowth8 %
119767NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836927897tryptophan-energy source
6836917632nitrate-reduction
11976716947citrate+carbon source
11976717632nitrate+reduction
11976716301nitrite-reduction
11976717234glucose+/-degradation
11976717632nitrate-respiration

antibiotic resistance

  • @ref: 119767
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11976735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11976715688acetoin-
11976717234glucose-

enzymes

@refvalueactivityec
2923catalase+1.11.1.6
2923cytochrome-c oxidase-1.9.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
119767oxidase-
119767beta-galactosidase-3.2.1.23
119767gelatinase+
119767catalase+1.11.1.6
119767gamma-glutamyltransferase-2.3.2.2
119767urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119767-+++++++--++-+------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2923--+/----+--------+-++--
2923--+---+--------+-++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119767------------------------------------------------++++-+--------++++-------+--++-++-------+-++-++-+--

Isolation, sampling and environmental information

isolation

@refsample type
2923human sputum
44295Human sputum
119767Human, Sputum

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Sputum

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
29232Risk group (German classification)
1197671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter haemolyticus 16S rRNA gene, partialAJ247200452ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 01HE651825580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 02HE651826649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 03HE651827669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 04HE651828580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 06HE651829649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 07HE651830580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 08HE651831669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 09HE651832614ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 10HE651833669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 11HE651834649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 12HE651835649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 13HE651836669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 14HE651837649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 15HE651838615ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 16HE651839649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 17HE651840580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 18HE651841580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 19HE651842669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 21HE651843669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 22HE651844580ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 23HE651845615ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 24HE651846649ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 25HE651847669ena29430
20218Acinetobacter haemolyticus genomic DNA containing 16S-23S IGS, strain ATCC 17906, clone 26HE651848614ena29430
20218Acinetobacter haemolyticus 16S rRNA gene, strain ATCC 17906HE6519151530ena29430
20218Acinetobacter haemolyticus 16S rRNA gene (strain ATCC 17906T)Z934371417ena29430
2923A.haemolyticus 16S rRNA gene (DSM6962)X816621460ena29430

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter haemolyticus CIP 64.3 = MTCC 9819GCA_000369065scaffoldncbi1217659
66792Acinetobacter haemolyticus CIP 64.3 = MTCC 9819GCA_000430205contigncbi1217659
66792Acinetobacter haemolyticus NCTC10305GCA_900444835contigncbi29430
66792Acinetobacter haemolyticus CIP 64.31217659.3wgspatric1217659
66792Acinetobacter haemolyticus strain FDAARGOS_139229430.120completepatric29430
66792Acinetobacter haemolyticus strain NCTC1030529430.62wgspatric29430
66792Acinetobacter haemolyticus ATCC 179062582581441draftimg1217659
66792Acinetobacter haemolyticus ATCC 179062537561554draftimg1217659

GC content

  • @ref: 2923
  • GC-content: 43.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.025no
anaerobicno98.383no
halophileno86.876no
spore-formingno96.409no
glucose-utilno86.675yes
motileno91.621no
flagellatedno93.099no
aerobicyes94.503no
thermophileno99.84no
glucose-fermentno90.474no

External links

@ref: 2923

culture collection no.: DSM 6962, ATCC 17906, CIP 64.3, NCTC 10305, CCUG 888, KCTC 12404, CCM 2358, LMG 996, NCIMB 12458

straininfo link

  • @ref: 77519
  • straininfo: 389266

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism8148619Structure of acinetoferrin, a new citrate-based dihydroxamate siderophore from Acinetobacter haemolyticus.Okujo N, Sakakibara Y, Yoshida T, Yamamoto SBiometals10.1007/BF001404881994Acinetobacter/*chemistry/growth & development/metabolism, Citrates/analysis, Citric Acid, Culture Media, Hydroxamic Acids/*chemistry/isolation & purification/metabolism, Iron/metabolism, Magnetic Resonance Spectroscopy, Molecular Structure, Siderophores/biosynthesis/*chemistry/isolation & purification, Spectrometry, Mass, Fast Atom Bombardment, Tricarboxylic Acids/*chemistry/isolation & purification/metabolismEnzymology
Phylogeny9108245Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter haemolyticus strain ATCC 17906.Haseley SR, Holst O, Brade HEur J Biochem10.1111/j.1432-1033.1997.00761.x1997Acinetobacter/classification/*immunology/isolation & purification, Animals, Antibodies, Bacterial, Carbohydrate Sequence, Humans, Immunization, Immunochemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, O Antigens/*chemistry, Rabbits, Serotyping, Species SpecificityPathogenicity
9249012Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4).Vinogradov EV, Muller-Loennies S, Petersen BO, Meshkov S, Thomas-Oates JE, Holst O, Brade HEur J Biochem10.1111/j.1432-1033.1997.00082.x1997Acinetobacter/*chemistry, Lipopolysaccharides/*chemistry, Mass Spectrometry, Oligosaccharides/analysis
Enzymology9660198Cloning and characterization of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase genes (kdtA) from Acinetobacter baumannii and Acinetobacter haemolyticus.Bode CE, Brabetz W, Brade HEur J Biochem10.1046/j.1432-1327.1998.2540404.x1998Acinetobacter/*enzymology/*genetics, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Corynebacterium/genetics, DNA Primers/genetics, Escherichia coli/genetics, *Genes, Bacterial, Genetic Vectors, Molecular Sequence Data, Phylogeny, Plasmids/genetics, Recombinant Proteins/genetics/metabolism, Sequence Homology, Amino Acid, Transferases/*genetics/metabolismGenetics
Metabolism23378574Identification and characterization of a cluster of genes involved in biosynthesis and transport of acinetoferrin, a siderophore produced by Acinetobacter haemolyticus ATCC 17906T.Funahashi T, Tanabe T, Maki J, Miyamoto K, Tsujibo H, Yamamoto SMicrobiology (Reading)10.1099/mic.0.065177-02013Acinetobacter/genetics/*metabolism, Amino Acid Sequence, Bacterial Proteins/genetics/*metabolism, Base Sequence, Biological Transport/genetics, Citrates/*biosynthesis/chemistry/*metabolism, *Gene Expression Regulation, Bacterial, Genes, Bacterial, Hydroxamic Acids/chemistry/*metabolism, Iron/metabolism, Molecular Sequence Data, *Multigene Family, Mutation, Sequence Analysis, DNA, Siderophores/biosynthesis/metabolismGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2923Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6962)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6962
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42153Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10102
44295Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 888)https://www.ccug.se/strain?id=888
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
77519Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389266.1StrainInfo: A central database for resolving microbial strain identifiers
119767Curators of the CIPCollection of Institut Pasteur (CIP 64.3)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2064.3