Strain identifier

BacDive ID: 8086

Type strain: Yes

Species: Acinetobacter junii

Strain Designation: 64.5

Strain history: CIP <- 1964, W. Mannheim, Hyg. Inst., Marburg, Germany: strain 2723/59, "Achromobacter citroalcaligenes"

NCBI tax ID(s): 40215 (species)

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General

@ref: 2925

BacDive-ID: 8086

DSM-Number: 6964

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative

description: Acinetobacter junii 64.5 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from human urine.

NCBI tax id

  • NCBI tax id: 40215
  • Matching level: species

strain history

@refhistory
2925<- ATCC <- NCTC <- W. Stenzel; 2723/59
421551964, W. Mannheim, Hyg. Inst., Marburg, Germany: strain 2723/59, "Achromobacter citroalcaligenes"
120367CIP <- 1964, W. Mannheim, Hyg. Inst., Marburg, Germany: strain 2723/59, "Achromobacter citroalcaligenes"

doi: 10.13145/bacdive8086.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter junii
  • full scientific name: Acinetobacter junii Bouvet and Grimont 1986

  • synonyms

    • @ref: 20215
    • synonym: Acinetobacter grimontii

@ref: 2925

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter junii

full scientific name: Acinetobacter junii Bouvet and Grimont 1986

strain designation: 64.5

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.915
120367negativerod-shapedno

colony morphology

  • @ref: 120367
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2925CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
42155MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120367CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
2925positivegrowth28mesophilic
42155positivegrowth30mesophilic
120367positivegrowth30-41
120367nogrowth10psychrophilic
120367nogrowth25mesophilic
120367nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120367
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.915

halophily

@refsaltgrowthtested relationconcentration
120367NaClpositivegrowth0-4 %
120367NaClnogrowth6 %
120367NaClnogrowth8 %
120367NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12036716947citrate+carbon source
12036717632nitrate+reduction
12036716301nitrite-reduction
12036717632nitrate-respiration

antibiotic resistance

  • @ref: 120367
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12036735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
12036715688acetoin-
12036717234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120367oxidase-
120367beta-galactosidase-3.2.1.23
120367gelatinase-
120367catalase+1.11.1.6
120367gamma-glutamyltransferase-2.3.2.2
120367urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120367--++++-+--++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2925---------------+--+--
2925---------------+--+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120367-----------------------------------------------------+-------------------+-+++-++-------+-++++--++-

Isolation, sampling and environmental information

isolation

@refsample type
2925human urine
44296Human urine
120367Human, Urine

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Urine

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
29252Risk group (German classification)
1203671Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter junii 16S rRNA gene, partialAJ247204452ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, direct sequence AllHE651782707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 01HE651783707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 02HE651784707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 03HE651785707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 05HE651786707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 06HE651787707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 07HE651788707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 09HE651789707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 10HE651790707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 11HE651791707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 12HE651792707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 13HE651793707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 14HE651794707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain ATCC 17908, clone 15HE651795707ena40215
20218Acinetobacter junii 16S rRNA gene, strain ATCC 17908HE6519161529ena40215
20218Acinetobacter junii 16S rRNA gene (strain ATCC 17908T)Z934381416ena40215
2925A.junii 16S rRNA gene (DSM6964)X816641459ena40215

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter junii NCTC10307GCA_900444875contigncbi40215
66792Acinetobacter junii strain NCTC1030740215.64wgspatric40215

GC content

  • @ref: 2925
  • GC-content: 42.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.074no
anaerobicno98.852no
halophileno89.036no
spore-formingno96.602no
glucose-utilno86.68yes
aerobicyes95.66no
flagellatedno92.199no
thermophileno99.892yes
motileno89.847no
glucose-fermentno91.512no

External links

@ref: 2925

culture collection no.: DSM 6964, ATCC 17908, NCTC 10307, CCUG 889, KCTC 12406, CIP 64.5, CCM 2376, LMG 998, NCIMB 12459

straininfo link

  • @ref: 77513
  • straininfo: 389268

literature

  • topic: Phylogeny
  • Pubmed-ID: 25801684
  • title: The impact of horizontal gene transfer on targeting the internal transcribed spacer region (ITS) to identify Acinetobacter junii strains.
  • authors: Maslunka C, Gurtler V, Seviour RJ
  • journal: J Appl Microbiol
  • DOI: 10.1111/jam.12800
  • year: 2015
  • mesh: Acinetobacter/classification/*genetics/isolation & purification, Base Sequence, DNA, Intergenic/*genetics, *Gene Transfer, Horizontal, Genetic Variation, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Reproducibility of Results
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2925Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6964)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6964
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42155Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10104
44296Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 889)https://www.ccug.se/strain?id=889
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77513Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389268.1StrainInfo: A central database for resolving microbial strain identifiers
120367Curators of the CIPCollection of Institut Pasteur (CIP 64.5)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2064.5