Strain identifier

BacDive ID: 8058

Type strain: Yes

Species: Acinetobacter lwoffii

Strain history: CIP <- 2013, NCTC

NCBI tax ID(s): 981327 (strain), 28090 (species)

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General

@ref: 1076

BacDive-ID: 8058

DSM-Number: 2403

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Acinetobacter lwoffii DSM 2403 is a mesophilic bacterium that was isolated from Urine.

NCBI tax id

NCBI tax idMatching level
28090species
981327strain

strain history

@refhistory
416242013, NCTC
1076<- ATCC <- NCTC <- A. Lwoff
67770ATCC 15309 <-- NCTC 5866 <-- A. Lwoff.
120715CIP <- 2013, NCTC

doi: 10.13145/bacdive8058.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter lwoffii
  • full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Moraxella lwoffi

@ref: 1076

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter lwoffii

full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 emend. Nemec et al. 2019

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.518
6948099.972negative
120715nonegativeoval-shaped

colony morphology

  • @ref: 1076
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41624MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
1076COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
1076NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
1076CASO AGAR (MERCK 105458) (DSMZ Medium 220)yesName: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/220
68368MacConkey mediumyes
120715CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
41624positivegrowth30mesophilic
1076positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

antibiogram

@refmediumPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactamincubation temperatureincubation timeoxygen condition
1076Mueller-Hinton Agar24-26830342214-161628-302240323228-303216241030301820-22162218-2040-42243426-2820183216-18020-22032
1076Mueller-Hinton Agar24-26830342220163020-2240-423034-362628-30142610322818201420183024-2632-3428201630-3220020026301aerob

spore formation

@refspore formationconfidence
69481no100
69480no99.996

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
6836817632nitrate-reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
1076catalase+1.11.1.6
1076cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOXNO2N2MAC
1076--------------------+/--+/-+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
1076--------+/-------+-+-+-
1076---------------+---+
1076---------------+++-+

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Urine

taxonmaps

  • @ref: 69479
  • File name: preview.99_43.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_43&stattab=map
  • Last taxonomy: Acinetobacter
  • 16S sequence: X81665
  • Sequence Identity:
  • Total samples: 10947
  • soil counts: 822
  • aquatic counts: 2946
  • animal counts: 6831
  • plant counts: 348

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
10762Risk group (German classification)
1207151Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter lwoffii 16S rRNA gene, partialAJ247205452ena28090
20218A.lwoffii gene for 16S rRNAX74894730ena981327
1076Acinetobacter lwoffii gene for 16S rRNA, partial sequence, strain: JCM 6840AB6261251460ena28090
67770A.lwoffii 16S rRNA gene (DSM2403)X816651460ena28090

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter lwoffii NCTC 5866 = CIP 64.10 = NIPH 512GCA_000487975scaffoldncbi981327
66792Acinetobacter lwoffii NCTC 5866 = CIP 64.10981327.3wgspatric981327
66792Acinetobacter lwoffii NCTC 5866 = CIP 64.10981327.6wgspatric981327
66792Acinetobacter lwoffii strain FDAARGOS 139328090.133completepatric28090
66792Acinetobacter lwoffii strain NBRC 10976028090.13wgspatric28090
66792Acinetobacter lwoffii strain NCTC586628090.96completepatric28090
66792Acinetobacter lwoffii NCTC 58662537562009draftimg981327
66792Acinetobacter lwoffii NCTC 58662547132300draftimg981327
66792Acinetobacter lwoffii NBRC 1097602660238430draftimg28090
67770Acinetobacter lwoffii NCTC 5866 = CIP 64.10 = NIPH 512GCA_000248355contigncbi981327
67770Acinetobacter lwoffii NBRC 109760GCA_000836095contigncbi28090
67770Acinetobacter lwoffii NCTC5866GCA_900699155contigncbi28090

GC content

  • @ref: 67770
  • GC-content: 46
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno91.296no
gram-positiveno97.24no
anaerobicno98.682no
halophileno83.618no
spore-formingno96.717no
glucose-utilno89.071no
flagellatedno96.11no
aerobicyes92.107no
thermophileno99.82yes
glucose-fermentno93.304yes

External links

@ref: 1076

culture collection no.: DSM 2403, ATCC 15309, CIP 644.10, JCM 6840, NCTC 5866, ATCC 17986, CCM 5581, CCUG 12805, CCUG 33984, CECT 453, CIP 64.10, LMG 1029, LMG 1240, NBRC 109760, NCIMB 12456, CIP 110687, KCTC 12407, NCIB 12456

straininfo link

  • @ref: 77486
  • straininfo: 229

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3210151Taxonomic studies of Acinetobacter species based on the electrophoretic analysis of enzymes.Nishimura Y, Kanzaki H, Iizuka HJ Basic Microbiol10.1002/jobm.36202806051988Acinetobacter/*classification/enzymology, Electrophoresis, Polyacrylamide Gel, Enzymes/*analysis, Gossypium, *Soil MicrobiologyPhylogeny
Enzymology22205817Genes encoding OXA-134-like enzymes are found in Acinetobacter lwoffii and A. schindleri and can be used for identification.Turton JF, Hyde R, Martin K, Shah JJ Clin Microbiol10.1128/JCM.06173-112012Acinetobacter/*classification/enzymology/*genetics, DNA Primers/genetics, DNA, Bacterial/genetics, Humans, Molecular Sequence Data, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis, DNA, Sequence Deletion, beta-Lactamases/*geneticsPathogenicity
Phylogeny24478215Acinetobacter harbinensis sp. nov., isolated from river water.Li W, Zhang D, Huang X, Qin WInt J Syst Evol Microbiol10.1099/ijs.0.055251-02014Acinetobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNAGenetics
Phylogeny30392743Revising the taxonomy of the Acinetobacter lwoffii group: The description of Acinetobacter pseudolwoffii sp. nov. and emended description of Acinetobacter lwoffii.Nemec A, Radolfova-Krizova L, Maixnerova M, Nemec M, Clermont D, Bzdil J, Jezek P, Spanelova PSyst Appl Microbiol10.1016/j.syapm.2018.10.0042018Acinetobacter/*classification, Bacterial Typing Techniques, DNA-Directed RNA Polymerases/genetics, Genes, Bacterial, *Phylogeny, Whole Genome SequencingGenetics
Phylogeny32725341Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey.Acer O, Guven K, Poli A, Di Donato P, Leone L, Buono L, Guven RG, Nicolaus B, Finore ICurr Microbiol10.1007/s00284-020-02134-92020*Acinetobacter/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, *Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, TurkeyTranscriptome
Phylogeny32845831The status of the genus Prolinoborus (Pot et al. 1992) and the species Prolinoborus fasciculus (Pot et al. 1992).Glaeser SP, Pulami D, Blom J, Eisenberg T, Goesmann A, Bender J, Wilharm G, Kampfer PInt J Syst Evol Microbiol10.1099/ijsem.0.0044042020Acinetobacter/*classification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Neisseriaceae/*classification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTranscriptome
Phylogeny33136203Strain "Acinetobacter mesopotamicus" GC2 Does Not Represent a Novel Species, but Belongs to the Species Acinetobacter lwoffii as Revealed by Whole-Genome Sequence-Based Analysis.Nemec ACurr Microbiol10.1007/s00284-020-02275-x2020*Acinetobacter/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Fatty Acids, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1076Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2403)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2403
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41624Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30492
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77486Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID229.1StrainInfo: A central database for resolving microbial strain identifiers
120715Curators of the CIPCollection of Institut Pasteur (CIP 110687)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110687