Strain identifier

BacDive ID: 8046

Type strain: Yes

Species: Plantactinospora siamensis

Strain Designation: CM2-8

Strain history: C. Thawai CM2-8.

NCBI tax ID(s): 555372 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17645

BacDive-ID: 8046

DSM-Number: 45559

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Plantactinospora siamensis CM2-8 is an aerobe, spore-forming, mesophilic bacterium that was isolated from temperate peat swamp forest soil.

NCBI tax id

  • NCBI tax id: 555372
  • Matching level: species

strain history

@refhistory
17645<- JCM <- C. Thawai; CM2-8
67770C. Thawai CM2-8.

doi: 10.13145/bacdive8046.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Plantactinospora
  • species: Plantactinospora siamensis
  • full scientific name: Plantactinospora siamensis (Thawai et al. 2010) Zhu et al. 2012
  • synonyms

    • @ref: 20215
    • synonym: Actinaurispora siamensis

@ref: 17645

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Plantactinospora

species: Plantactinospora siamensis

full scientific name: Plantactinospora siamensis (Thawai et al. 2010) Zhu et al. 2012

strain designation: CM2-8

type strain: yes

Morphology

cell morphology

  • @ref: 29470
  • gram stain: positive
  • cell width: 0.4-0.6 µm
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
21398Yellow orange (2000)10-14 daysISP 2
21398Golden yellow (1004)10-14 daysISP 3
21398Saffron yellow (1017)10-14 daysISP 4
21398Saffron yellow (1017)10-14 daysISP 5
21398Bright red orange (2008)10-14 daysISP 7
21398Brown beige (1011)10-14 daysSuter with tyrosine
21398Beige (1001)10-14 daysSuter without tyrosine

multicellular morphology

@refforms multicellular complexmedium name
21398noISP 2
21398noISP 3
21398noISP 4
21398noISP 5
21398noISP 7
21398noSuter with tyrosine
21398noSuter without tyrosine

pigmentation

  • @ref: 29470
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17645N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
17645GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
21398ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
21398ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
21398ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
21398ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
21398ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
21398Suter with tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes
21398Suter without tyrosineyesName: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
17645positivegrowth28mesophilic
29470positivegrowth25-40
29470positiveoptimum25-30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
29470positivegrowth05-07
29470positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 29470
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29470
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
21398NaClpositivegrowth0 %
29470NaClpositivegrowth0-1.5 %
29470NaClpositiveoptimum0-1.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-10(H6), MK-9(H8), MK-10(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2139817234glucose+
2139822599arabinose+
2139817992sucrose+
2139818222xylose+
2139817268myo-inositol+
2139837684mannose+
2139828757fructose+
2139826546rhamnose+
2139816634raffinose+
2139862968cellulose+
2947017057cellobiose+carbon source
2947028757fructose+carbon source
2947028260galactose+carbon source
2947017234glucose+carbon source
2947017716lactose+carbon source
2947028053melibiose+carbon source
2947026546rhamnose+carbon source
2947018222xylose+carbon source
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
21398-+-----------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21398+++/--+--+-+-++-+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17645temperate peat swamp forest soilChiang Mai ProvinceThailandTHAAsia
67770Temperate peat swamp forest soilChiang Mai ProvinceThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Wetland (Swamp)

Safety information

risk assessment

  • @ref: 17645
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17645
  • description: Actinaurispora siamensis gene for 16S rRNA, partial sequence, strain: CM2-8
  • accession: AB454379
  • length: 1446
  • database: ena
  • NCBI tax ID: 555372

GC content

@refGC-contentmethod
1764572.6high performance liquid chromatography (HPLC)
2947072.6

External links

@ref: 17645

culture collection no.: DSM 45559, BCC 34762, JCM 15677, NBRC 109072

straininfo link

  • @ref: 77474
  • straininfo: 405668

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19734290Actinaurispora siamensis gen. nov., sp. nov., a new member of the family Micromonosporaceae.Thawai C, Tanasupawat S, Suwanborirux K, Kudo TInt J Syst Evol Microbiol10.1099/ijs.0.013763-02009Base Sequence, Micromonosporaceae/*classification/genetics/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny22140153Plantactinospora endophytica sp. nov., an actinomycete isolated from Camptotheca acuminata Decne., reclassification of Actinaurispora siamensis as Plantactinospora siamensis comb. nov. and emended descriptions of the genus Plantactinospora and Plantactinospora mayteni.Zhu WY, Zhao LX, Zhao GZ, Duan XW, Qin S, Li J, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.036459-02011Bacterial Typing Techniques, Base Composition, Camptotheca/*microbiology, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17645Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45559)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45559
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
21398Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45559.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
29470Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2587328776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
77474Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405668.1StrainInfo: A central database for resolving microbial strain identifiers