Strain identifier

BacDive ID: 8045

Type strain: Yes

Species: Plantactinospora mayteni

Strain history: DSM 45238 <-- S.-K. Tang <-- S. Qin et al. YIM 61359.

NCBI tax ID(s): 566021 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15913

BacDive-ID: 8045

DSM-Number: 45238

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Plantactinospora mayteni DSM 45238 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized roots of Maytenus austroyunnanensis collected from tropical rainforest.

NCBI tax id

  • NCBI tax id: 566021
  • Matching level: species

strain history

@refhistory
15913<- S.-K. Tang, YIM; YIM 61359 <- S. Qin and et al.
67770DSM 45238 <-- S.-K. Tang <-- S. Qin et al. YIM 61359.

doi: 10.13145/bacdive8045.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Plantactinospora
  • species: Plantactinospora mayteni
  • full scientific name: Plantactinospora mayteni Qin et al. 2009

@ref: 15913

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Plantactinospora

species: Plantactinospora mayteni

full scientific name: Plantactinospora mayteni Qin et al. 2009 emend. Zhu et al. 2012

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
29317positiveno
69480no94.668
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
19927Golden yellow10-14 daysISP 2
19927Golden yellow10-14 daysISP 3
19927Grey olive10-14 daysISP 4
19927Signal white10-14 daysISP 5
19927Zinc yellow10-14 daysISP 6
19927Cream10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19927yesAerial MyceliumLight ivoryISP 2
19927noISP 3
19927noISP 4
19927noISP 5
19927noISP 6
19927noISP 7

pigmentation

  • @ref: 29317
  • production: no

multimedia

  • @ref: 15913
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45238.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15913TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
15913GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19927ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19927ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19927ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19927ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19927ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19927ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
15913GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15913positivegrowth28mesophilic
19927positiveoptimum28mesophilic
29317positivegrowth15-37
29317positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
29317positivegrowth6.0-8.0
29317positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 29317
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
29317yes
69481yes100
69480yes100

halophily

  • @ref: 29317
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 4 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H6), MK-10(H8), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1992717234glucose+
1992722599arabinose+/-
1992717992sucrose+/-
1992718222xylose-
1992717268myo-inositol-
1992729864mannitol+/-
1992728757fructose+/-
1992726546rhamnose+/-
1992716634raffinose-
1992762968cellulose-
2931722599arabinose+carbon source
2931717057cellobiose+carbon source
2931728757fructose+carbon source
2931728260galactose+carbon source
2931717234glucose+carbon source
2931717716lactose+carbon source
2931717306maltose+carbon source
2931729864mannitol+carbon source
2931737684mannose+carbon source
2931716634raffinose+carbon source
2931726546rhamnose+carbon source
2931733942ribose+carbon source
2931730911sorbitol+carbon source
2931717992sucrose+carbon source
2931727082trehalose+carbon source
2931718222xylose+carbon source
2931717632nitrate+reduction

metabolite production

  • @ref: 29317
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19927+++++++++-+-+-+++--

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
15913surface-sterilized roots of Maytenus austroyunnanensis collected from tropical rainforestMaytenus austroyunnanensisYunnan Province, XishuangbannaChinaCHNAsia
67770Surface-sterilized roots of Maytenus austroyunnanensis from a tropical rainforestMaytenus austroyunnanensisXishuangbanna, Yunnan Province, south-west ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root (Rhizome)
#Host Body-Site#Plant#Sterilized plant part
#Climate#Hot#Tropical

Safety information

risk assessment

  • @ref: 15913
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15913
  • description: Plantactinospora mayteni strain YIM 61359 16S ribosomal RNA gene, partial sequence
  • accession: FJ214343
  • length: 1427
  • database: ena
  • NCBI tax ID: 566021

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Plantactinospora mayteni NBRC 109088GCA_016862935contigncbi566021
66792Plantactinospora mayteni strain NBRC 109088566021.3wgspatric566021

GC content

@refGC-contentmethod
1591369.7high performance liquid chromatography (HPLC)
2931769.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes88.95yes
anaerobicno99.131yes
halophileno94.599no
spore-formingyes94.835yes
glucose-utilyes89.162yes
thermophileno98.272yes
flagellatedno97.53yes
motileno93.225yes
aerobicyes94.507yes
glucose-fermentno91.665no

External links

@ref: 15913

culture collection no.: DSM 45238, BCC 55027, CCTCC AA 208022, JCM 16905, NBRC 109088, TBRC 2210, YIM 61359

straininfo link

  • @ref: 77473
  • straininfo: 396846

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19622648Plantactinospora mayteni gen. nov., sp. nov., a member of the family Micromonosporaceae.Qin S, Li J, Zhang YQ, Zhu WY, Zhao GZ, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.010793-02009Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Lipids/chemistry, Maytenus/*microbiology, Micromonosporaceae/chemistry/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, Spores, Bacterial/cytologyGenetics
Phylogeny21873512Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora.Xi L, Zhang L, Ruan J, Huang YInt J Syst Evol Microbiol10.1099/ijs.0.033787-02011Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, China, Cluster Analysis, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Micromonosporaceae/*classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, WetlandsGenetics
Phylogeny22140153Plantactinospora endophytica sp. nov., an actinomycete isolated from Camptotheca acuminata Decne., reclassification of Actinaurispora siamensis as Plantactinospora siamensis comb. nov. and emended descriptions of the genus Plantactinospora and Plantactinospora mayteni.Zhu WY, Zhao LX, Zhao GZ, Duan XW, Qin S, Li J, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.036459-02011Bacterial Typing Techniques, Base Composition, Camptotheca/*microbiology, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics
Phylogeny25747424Plantactinospora veratri sp. nov., an actinomycete isolated from black false hellebore root (Veratrum nigrum L.).Xing H, Liu C, Zhang Y, Zhao J, Li C, Liu H, Li L, Wang X, Xiang WInt J Syst Evol Microbiol10.1099/ijs.0.0001802015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Veratrum/*microbiologyGenetics
Phylogeny26446200Plantactinospora sonchi sp. nov., an actinobacterium isolated from the leaves of common sowthistle (Sonchus oleraceus L.).Ma Z, Liu C, Fan J, He H, Li C, Li J, Zhao S, Xiang W, Wang XInt J Syst Evol Microbiol10.1099/ijsem.0.0006682015Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sonchus/*microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15913Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45238)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45238
19927Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45238.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29317Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2573028776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77473Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID396846.1StrainInfo: A central database for resolving microbial strain identifiers