Strain identifier

BacDive ID: 7963

Type strain: Yes

Species: Micromonospora equina

Strain Designation: Y22

Strain history: DSM 45644 <-- P. Meyers; Univ. Cape Town, South Africa; Y22.

NCBI tax ID(s): 1043168 (species)

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General

@ref: 17968

BacDive-ID: 7963

DSM-Number: 45644

keywords: 16S sequence, Bacteria, mesophilic, Gram-positive

description: Micromonospora equina Y22 is a mesophilic, Gram-positive bacterium that was isolated from soil from a fynbos-rich area.

NCBI tax id

  • NCBI tax id: 1043168
  • Matching level: species

strain history

@refhistory
17968<- P. Meyers, Univ. Cape Town, South Africa; Y22 <- G. Everest
67770DSM 45644 <-- P. Meyers; Univ. Cape Town, South Africa; Y22.

doi: 10.13145/bacdive7963.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Micromonospora
  • species: Micromonospora equina
  • full scientific name: Micromonospora equina Everest and Meyers 2013

@ref: 17968

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Micromonospora

species: Micromonospora equina

full scientific name: Micromonospora equina Everest and Meyers 2013

strain designation: Y22

type strain: yes

Morphology

cell morphology

  • @ref: 30718
  • gram stain: positive

colony morphology

@refcolony colormedium used
69414Daffodil yellow (1007)ISP 2
69414Beige (1001), signal yellow (1003)suter without tyrosine
69414Beige (1001), dahlia yellow (1033)suter with tyrosine
69414Light ivory (1015)ISP 4
69414Light ivory (1015)ISP 5
69414Light ivory (1015), pastel orange (2003)ISP 7
69414Sand yellow (1002)ISP 6
69414Yellow orange (2000)ISP 3

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69414noAerial myceliumISP 2
69414noAerial myceliumISP 3
69414noAerial myceliumISP 4
69414noAerial myceliumISP 5
69414noAerial myceliumISP 6
69414noAerial myceliumISP 7
69414noAerial myceliumsuter with tyrosine
69414noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
30718yes
69414noMelanin
69414nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
17968https://www.dsmz.de/microorganisms/photos/DSM_45644.jpgMedium 554 28°C© Leibniz-Institut DSMZ
69414DSM_45644_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69414DSM_45644_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17968GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
17968N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
17968GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
17968TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17968positivegrowth28mesophilic
30718positivegrowth20-37
67770positivegrowth28mesophilic

culture pH

  • @ref: 30718
  • ability: positive
  • type: growth
  • pH: 05-10
  • PH range: alkaliphile

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
30718NaClpositivegrowth0-2 %
69414NaClpositivegrowth0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H4), MK-9(H4), MK-10(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6941422599arabinose+growth
3071822599arabinose+carbon source
3071817234glucose+carbon source
3071817716lactose+carbon source
3071817306maltose+carbon source
3071828053melibiose+carbon source
3071827082trehalose+carbon source
307184853esculin+hydrolysis
3071817632nitrate+reduction
6941462968cellulose+growth
6941428757fructose+growth
6941417234glucose+growth
6941417268inositol+growth
6941437684mannose+growth
6941416634raffinose+growth
6941426546rhamnose+growth
6941417992sucrose+growth
6941418222xylose+growth
6837917632nitrate-reduction
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 30718
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
30718gelatinase+
68379gelatinase+
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69414---+--+++/-+/-+---+/-----

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69414++/-++/-++/-+/-+/-++++/-+/-+/-+++-+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17968soil from a fynbos-rich areaCape Town, Kenilworth RacecourseSouth AfricaZAFAfrica
67770Horse racecourseCape TownSouth AfricaZAFAfrica

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 17968
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17968
  • description: Micromonospora equina strain Y22 16S ribosomal RNA gene, partial sequence
  • accession: JF912511
  • length: 1375
  • database: ena
  • NCBI tax ID: 1043168

External links

@ref: 17968

culture collection no.: DSM 45644, JCM 31035, NRRL B-24859

straininfo link

  • @ref: 77397
  • straininfo: 397199

literature

  • topic: Phylogeny
  • Pubmed-ID: 22634700
  • title: Micromonospora equina sp. nov., isolated from soil from a racecourse.
  • authors: Everest GJ, Meyers PR
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.042929-0
  • year: 2012
  • mesh: Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Horses, Micromonospora/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, South Africa
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17968Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45644)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45644
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30718Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2704928776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69414Wink, J.https://cdn.dsmz.de/wink/DSM%2045644.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
77397Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397199.1StrainInfo: A central database for resolving microbial strain identifiers