Strain identifier
BacDive ID: 790
Type strain: 
Species: Peribacillus simplex
Strain Designation: NRS-960
Strain history: CIP <- 2001, CCUG <- 1991, L.K. Nakamura, NRRL, Peoria, USA: strain NRS-960
NCBI tax ID(s): 1478 (species)
version 7
General
@ref: 519
BacDive-ID: 790
DSM-Number: 1321
keywords: mesophilic, Bacteria, 16S sequence, genome sequence
description: Peribacillus simplex DSM 1321 is a mesophilic bacterium that was isolated from Soil.
NCBI tax id
- NCBI tax id: 1478
- Matching level: species
strain history
| <- R.E. Gordon, NRS 960 (Bacillus megaterium) <- J.R. Porter <- O.F. Edwards <- H.J. Cohn |
| DSM 1321 <-- R. E. Gordon NRS 960 <-- J. R. Porter <-- O. F. Edwards <-- H. J. Cohn. |
doi: 10.13145/bacdive790.20220920.7
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Peribacillus
- species: Peribacillus simplex
- full scientific name: Peribacillus simplex (Priest et al. 1989) Patel and Gupta 2020
synonyms
- @ref: 20215
- synonym: Bacillus simplex
@ref: 519
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Peribacillus
species: Peribacillus simplex
full scientific name: Peribacillus simplex (Priest et al. 1989) Patel and Gupta 2020
type strain: yes
Morphology
colony morphology
- @ref: 22903
- colony color: white
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 22903 | MacConkey agar | yes | ||
| 519 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://bacmedia.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| 34193 | MEDIUM 29- Brain heart agar | yes | Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g) |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 519 | positive | growth | 30 | mesophilic |
| 34193 | positive | growth | 30 | mesophilic |
| 67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 22903 | 28757 | fructose | - | builds acid from |
| 22903 | 28260 | galactose | - | builds acid from |
| 22903 | 5291 | gelatin | - | hydrolysis |
| 22903 | 17234 | glucose | + | builds acid from |
| 22903 | 17754 | glycerol | + | builds acid from |
| 22903 | 17716 | lactose | - | builds acid from |
| 22903 | 17306 | maltose | + | builds acid from |
| 22903 | 29864 | mannitol | + | builds acid from |
| 22903 | 37684 | mannose | + | builds acid from |
| 22903 | 16634 | raffinose | - | builds acid from |
| 22903 | 30911 | sorbitol | + | builds acid from |
| 22903 | 17992 | sucrose | - | builds acid from |
| 22903 | 27082 | trehalose | + | builds acid from |
| 22903 | 18222 | xylose | + | builds acid from |
| 22903 | 17632 | nitrate | + | reduction |
| 22904 | casein | + | hydrolysis | |
| 22904 | 17634 | D-glucose | + | builds acid from |
| 22904 | 16899 | D-mannitol | + | builds acid from |
| 22904 | 16024 | D-mannose | - | builds acid from |
| 22904 | 16988 | D-ribose | + | builds acid from |
| 22904 | 5291 | gelatin | + | hydrolysis |
| 22904 | 30849 | L-arabinose | - | builds acid from |
| 22904 | 17716 | lactose | - | builds acid from |
| 22904 | 28017 | starch | + | hydrolysis |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 22903 | acid phosphatase | + | 3.1.3.2 |
| 22903 | alkaline phosphatase | + | 3.1.3.1 |
| 22903 | alpha-chymotrypsin | + | 3.4.21.1 |
| 22903 | alpha-glucosidase | + | 3.2.1.20 |
| 22903 | beta-galactosidase | - | 3.2.1.23 |
| 22903 | beta-glucosidase | - | 3.2.1.21 |
| 22903 | cystine arylamidase | - | 3.4.11.3 |
| 22903 | gelatinase | - | |
| 22903 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 22903 | trypsin | - | 3.4.21.4 |
| 22903 | valine arylamidase | - | |
| 22904 | gelatinase | + |
Isolation, sampling and environmental information
isolation
- @ref: 67770
- sample type: Soil
Safety information
risk assessment
- @ref: 519
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Bacillus simplex clone Bsim-1 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AF478071 | 410 | ena | 1478 |
| 20218 | Bacillus simplex 16S rRNA gene, strain DSM 1321T | AJ439078 | 1522 | ena | 1478 |
| 20218 | Bacillus simplex DNA for 16S ribosomal RNA, partial sequence | D78478 | 1433 | ena | 1478 |
| 20218 | B.simplex 16S ribosomal RNA | X60638 | 1429 | ena | 1478 |
| 20218 | Bacillus simplex partial 16S rRNA gene, type strain LMG 11160T | AJ628743 | 1503 | ena | 1478 |
| 20218 | Bacillus simplex gene for 16S rRNA, partial sequence, strain: NBRC 15720 | AB363738 | 1476 | ena | 1478 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Peribacillus simplex NBRC 15720 = DSM 1321 DSM 1321 | GCA_002243645 | complete | ncbi | 1349754 |
| 66792 | Peribacillus simplex NBRC 15720 = DSM 1321 NBRC 15720 | GCA_001591785 | contig | ncbi | 1349754 |
| 66792 | Peribacillus simplex NBRC 15720 = DSM 1321 DE0084 | GCA_007679575 | scaffold | ncbi | 1349754 |
| 66792 | Bacillus simplex NBRC 15720 | 1349754.4 | wgs | patric | 1349754 |
| 66792 | Bacillus simplex NBRC 15720 = DSM 1321 | 1349754.5 | complete | patric | 1349754 |
| 66792 | Bacillus simplex NBRC 15720 = DSM 1321 strain DE0084 | 1349754.9 | wgs | patric | 1349754 |
| 66792 | Bacillus simplex DSM 1321 | 2823415468 | complete | img | 1349754 |
| 66792 | Bacillus simplex NBRC 15720 | 2731957667 | draft | img | 1349754 |
| 67770 | Peribacillus simplex NBRC 15720 = DSM 1321 strain NBRC 15720, whole genome shotgun sequencing project | BCVO00000000 | ncbi | 1349754 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 519 | 40.1 | |
| 67770 | 40-41 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 519
culture collection no.: DSM 1321, ATCC 49097, NRS 960, JCM 12307, BCRC 15909, CCM 7148, CCUG 28889, CGMCC 1.3471, CIP 106934, IFO 15720, KCTC 3450, LMG 11160, NBRC 15720, NRRL NRS-960, VTT E-97781
straininfo link
- @ref: 20218
- passport: http://www.straininfo.net/strains/5021
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 18218958 | Bacillus butanolivorans sp. nov., a species with industrial application for the remediation of n-butanol. | Kuisiene N, Raugalas J, Sproer C, Kroppenstedt RM, Chitavichius D | Int J Syst Evol Microbiol | 10.1099/ijs.0.65332-0 | 2008 | Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Butanols/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal Spacer/analysis, Fatty Acids/analysis, Genes, rRNA, Industrial Microbiology/methods, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity | Genetics |
| Phylogeny | 21441377 | Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. | Zhang YZ, Chen WF, Li M, Sui XH, Liu HC, Zhang XX, Chen WX | Int J Syst Evol Microbiol | 10.1099/ijs.0.028936-0 | 2011 | Bacillus/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Endophytes, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soybeans/*microbiology, Species Specificity, Vitamin K 2/analysis | Genetics |
| Phylogeny | 24903955 | Bacillus huizhouensis sp. nov., isolated from a paddy field soil. | Li J, Yang G, Wu M, Zhao Y, Zhou S | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0208-2 | 2014 | Aerobiosis, Anaerobiosis, Bacillus/*classification/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Oryza, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Spores, Bacterial/cytology, Temperature, Vitamin K 2/analysis | Genetics |
| Phylogeny | 25256951 | Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan. | Liu B, Liu GH, Sengonca C, Schumann P, Wang MK, Tang JY, Chen MC | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0284-3 | 2014 | Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Taiwan, Temperature | Genetics |
| Phylogeny | 26303844 | Bacillus gossypii sp. nov., isolated from the stem of Gossypium hirsutum. | Kampfer P, Busse HJ, McInroy JA, Glaeser SP | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000555 | 2015 | Alabama, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gossypium/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Plant Stems/*microbiology, Polyamines/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 27117173 | Bacillus loiseleuriae sp. nov., isolated from rhizosphere soil from a loiseleuria plant. | Liu B, Liu GH, Zhu YJ, Wang JP, Che JM, Chen QQ, Chen Z | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001107 | 2016 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Ericaceae/*microbiology, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Genetics | 27340061 | Draft Genome Sequence of Bacillus simplex DSM 1321 for Setting Up Phylogenomics in Genomic Taxonomy of the Bacillus-Like Bacteria. | Liu GH, Liu B, Wang JP, Che JM, Chen QQ, Chen Z | Genome Announc | 10.1128/genomeA.00574-16 | 2016 | Phylogeny | |
| Phylogeny | 32248281 | Reclassification of Brevibacterium frigoritolerans DSM 8801(T) as Bacillus frigoritolerans comb. nov. Based on Genome Analysis. | Liu GH, Liu B, Wang JP, Che JM, Li PF | Curr Microbiol | 10.1007/s00284-020-01964-x | 2020 | Bacillus/classification/*genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Genome, Bacterial, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Whole Genome Sequencing | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 519 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1321) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1321 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 22903 | Isao Yumoto, Kikue Hirota, Shingo Yamaga, Yoshinobu Nodasaka, Tsuneshirou Kawasaki, Hidetoshi Matsuyama, Kenji Nakajima | 10.1099/ijs.0.03014-0 | Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids | IJSEM 54: 1997-2001 2004 | 15545424 | |
| 22904 | Yun Zeng Zhang, Wen Feng Chen, Mao Li, Xin Hua Sui, Hong-Can Liu, Xiao Xia Zhang, Wen Xin Chen | 10.1099/ijs.0.028936-0 | Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root | IJSEM 62: 359-363 2012 | 21441377 | |
| 34193 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19119 | ||||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |