Strain identifier
BacDive ID: 7889
Type strain: 
Species: Micromonospora coerulea
Strain Designation: 36
Strain history: KCC A-0175 <-- IFO 13504 <-- ATCC 27008 <-- G. M. Luedemann 36.
NCBI tax ID(s): 47856 (species)
version 9.2 (current version)
General
@ref: 10814
BacDive-ID: 7889
DSM-Number: 43143
keywords: 16S sequence, Bacteria, mesophilic
description: Micromonospora coerulea 36 is a mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 47856
- Matching level: species
strain history
| @ref | history |
|---|---|
| 10814 | <- KCC <- G.M. Luedemann, 36 |
| 67770 | KCC A-0175 <-- IFO 13504 <-- ATCC 27008 <-- G. M. Luedemann 36. |
doi: 10.13145/bacdive7889.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micromonosporales
- family: Micromonosporaceae
- genus: Micromonospora
- species: Micromonospora coerulea
- full scientific name: Micromonospora coerulea Jensen 1932 (Approved Lists 1980)
@ref: 10814
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Micromonosporaceae
genus: Micromonospora
species: Micromonospora coerulea
full scientific name: Micromonospora coerulea Jensen 1932
strain designation: 36
type strain: yes
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19545 | Black red | 10-14 days | ISP 2 |
| 19545 | Fir green | 10-14 days | ISP 3 |
| 19545 | Salmon orange | 10-14 days | ISP 4 |
| 19545 | Fir green | 10-14 days | ISP 5 |
| 19545 | 10-14 days | ISP 6 | |
| 19545 | Fir green | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name | complex name | complex color |
|---|---|---|---|---|
| 19545 | no | ISP 2 | ||
| 19545 | no | ISP 3 | ||
| 19545 | no | ISP 4 | ||
| 19545 | yes | ISP 5 | Aerial Mycelium | White |
| 19545 | no | ISP 6 | ||
| 19545 | no | ISP 7 |
Culture and growth conditions
culture medium
| @ref | name | growth | composition | link |
|---|---|---|---|---|
| 19545 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19545 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19545 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19545 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19545 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19545 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 10814 | GYM+S MEDIUM (DSMZ Medium 214) | yes | Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | https://mediadive.dsmz.de/medium/214 |
| 10814 | BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) | yes | Name: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water | https://mediadive.dsmz.de/medium/215b |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 19545 | positive | optimum | 28 |
| 10814 | positive | growth | 28 |
| 67770 | positive | growth | 28 |
Physiology and metabolism
halophily
- @ref: 19545
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 5 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 19545 | 17234 | glucose | + | |
| 19545 | 22599 | arabinose | + | |
| 19545 | 17992 | sucrose | + | |
| 19545 | 18222 | xylose | + | |
| 19545 | 17268 | myo-inositol | + | |
| 19545 | 29864 | mannitol | + | |
| 19545 | 28757 | fructose | + | |
| 19545 | 26546 | rhamnose | + | |
| 19545 | 16634 | raffinose | + | |
| 19545 | 62968 | cellulose | - | |
| 68368 | 5291 | gelatin | + | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | - | hydrolysis |
| 68368 | 16947 | citrate | - | assimilation |
| 68368 | 18257 | ornithine | + | degradation |
| 68368 | 25094 | lysine | + | degradation |
| 68368 | 29016 | arginine | + | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | yes |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | + | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | urease | - | 3.5.1.5 |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 |
| 68368 | gelatinase | + | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | lysine decarboxylase | + | 4.1.1.18 |
| 68368 | arginine dihydrolase | + | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 19545 | + | + | + | + | - | - | - | - | - | + | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 10814 | soil | Hawaii | USA | USA | North America |
| 67770 | Soil, Mt. Heleakala | Maui Island, HI | USA | USA | North America |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 19545 | 1 | Risk group (German classification) |
| 10814 | 1 | Risk group (German classification) |
Sequence information
16S sequences
- @ref: 10814
- description: M.coerulea 16S rRNA gene
- accession: X92598
- length: 1476
- database: nuccore
- NCBI tax ID: 47856
GC content
- @ref: 67770
- GC-content: 71.7
- method: high performance liquid chromatography (HPLC)
External links
@ref: 10814
culture collection no.: DSM 43143, ATCC 27008, IFO 13504, KCC A-0175, NBRC 13504, JCM 3175, CBS 875.71, IFM 1280, IMET 8210, IMSNU 20084, KCTC 9368, NCIMB 12665, RIA 1560, VKM Ac-661
straininfo link
- @ref: 77324
- straininfo: 92510
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15046305 | Cryptoendolithic actinomycetes from antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932. | Hirsch P, Mevs U, Kroppenstedt RM, Schumann P, Stackebrandt E | Syst Appl Microbiol | 10.1078/072320204322881781 | 2004 | Antarctic Regions, Base Composition, Base Sequence, Carbohydrates/analysis, DNA, Bacterial/chemistry/genetics, Fatty Acids/analysis, Micromonospora/*classification/genetics/*isolation & purification/ultrastructure, Microscopy, Electron, Scanning, Microscopy, Phase-Contrast, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/chemistry/genetics, *Soil Microbiology | Genetics |
| Phylogeny | 23907218 | Micromonospora kangleipakensis sp. nov., isolated from a sample of limestone quarry. | Nimaichand S, Zhang YG, Cheng J, Li L, Zhang DF, Zhou EM, Dong L, Ningthoujam DS, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.052746-0 | 2013 | Bacterial Typing Techniques, Base Composition, *Calcium Carbonate, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Micromonospora/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry | Genetics |
| Phylogeny | 33666807 | Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin. | Sun X, Qiu S, Luo X, Jin P, Zhao J, Wu X, Yang J, Wang X, Song J, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01550-6 | 2021 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, *Micromonospora/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, Vitamin K 2 | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 10814 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43143) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43143 | |
| 19545 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM43143.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 68368 | Automatically annotated from API 20E | |||
| 77324 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92510.1 | StrainInfo: A central database for resolving microbial strain identifiers |