Strain identifier
BacDive ID: 7874
Type strain: 
Species: Dactylosporangium vinaceum
Strain Designation: SF-2127
Strain history: KCC A-0307 <-- Meiji Seika Kaisha, Ltd.; SF-2127.
NCBI tax ID(s): 53362 (species)
version 9 (current version)
General
@ref: 11294
BacDive-ID: 7874
DSM-Number: 43823
keywords: 16S sequence, Bacteria, antibiotic compound production
description: Dactylosporangium vinaceum SF-2127 is a bacterium that produces antibiotic compounds and was isolated from soil.
NCBI tax id
- NCBI tax id: 53362
- Matching level: species
strain history
| @ref | history |
|---|---|
| 11294 | <- IFO <- Meiji Seika Kaisha, SF-2127 |
| 67770 | KCC A-0307 <-- Meiji Seika Kaisha, Ltd.; SF-2127. |
doi: 10.13145/bacdive7874.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micromonosporales
- family: Micromonosporaceae
- genus: Dactylosporangium
- species: Dactylosporangium vinaceum
- full scientific name: Dactylosporangium vinaceum Shomura et al. 1983
@ref: 11294
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Micromonosporaceae
genus: Dactylosporangium
species: Dactylosporangium vinaceum
full scientific name: Dactylosporangium vinaceum Shomura et al. 1983
strain designation: SF-2127
type strain: yes
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19960 | Brown | 10-14 days | ISP 2 |
| 19960 | Beige (1001) | 10-14 days | ISP 3 |
| 19960 | Beige (1001) | 10-14 days | ISP 4 |
| 19960 | Red brown (8012) | 10-14 days | ISP 5 |
| 19960 | Beige (1001) | 10-14 days | ISP 6 |
| 19960 | Red brown (8012) | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name |
|---|---|---|
| 19960 | no | ISP 2 |
| 19960 | no | ISP 3 |
| 19960 | no | ISP 4 |
| 19960 | no | ISP 5 |
| 19960 | no | ISP 6 |
| 19960 | no | ISP 7 |
multimedia
- @ref: 11294
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43823.jpg
- caption: Medium 554 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 11294 | N-Z-AMINE-MEDIUM (DSMZ Medium 554) | yes | https://mediadive.dsmz.de/medium/554 | Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water |
| 19960 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19960 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19960 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19960 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19960 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19960 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 11294 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
| 11294 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 19960 | positive | optimum | 28 |
| 11294 | positive | growth | 28 |
| 67770 | positive | growth | 28 |
Physiology and metabolism
spore formation
- @ref: 19960
- spore description: Formation of sporangia, spore surface smooth
- spore formation: no
compound production
| @ref | compound |
|---|---|
| 11294 | dactimycin |
| 19960 | Dactimicin |
halophily
- @ref: 19960
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 2.5 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 19960 | 62968 | cellulose | - | |
| 19960 | 16634 | raffinose | + | |
| 19960 | 26546 | rhamnose | + | |
| 19960 | 28757 | fructose | + | |
| 19960 | 29864 | mannitol | + | |
| 19960 | 17268 | myo-inositol | - | |
| 19960 | 18222 | xylose | + | |
| 19960 | 17992 | sucrose | + | |
| 19960 | 22599 | arabinose | + | |
| 19960 | 17234 | glucose | + | |
| 68368 | 5291 | gelatin | + | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | - | hydrolysis |
| 68368 | 16947 | citrate | - | assimilation |
| 68368 | 18257 | ornithine | - | degradation |
| 68368 | 25094 | lysine | - | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 67770 | 81430 | dactimicin | yes |
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | gelatinase | + | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | - | 3.5.1.5 |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 |
| 68368 | lysine decarboxylase | - | 4.1.1.18 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | - | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 19960 | - | - | - | - | - | - | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 11294 | soil | ||||
| 67770 | Soil | Sekigahara, Gifu Pref. | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 11294 | 1 | Risk group (German classification) |
| 19960 | 1 | German classification |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | D.vinaceum 16S rRNA gene | X93196 | 1476 | nuccore | 53362 |
| 20218 | Dactylosporangium vinaceum gene for 16S ribosomal RNA, partial sequence | D86939 | 1465 | nuccore | 53362 |
External links
@ref: 11294
culture collection no.: DSM 43823, ATCC 35207, IFO 14181, JCM 3307, KCC A-0307, NBRC 14181, NCIMB 12891, BCRC 13327, HUT 6560, IMSNU 22056, KCTC 9359, NRRL B-16297, VKM Ac-1322
straininfo link
- @ref: 77310
- straininfo: 40923
literature
- topic: Biotechnology
- Pubmed-ID: 34189614
- title: Rubrolone production by Dactylosporangium vinaceum: biosynthesis, modulation and possible biological function.
- authors: Moureu S, Caradec T, Trivelli X, Drobecq H, Beury D, Bouquet P, Caboche S, Desmecht E, Maurier F, Muharram G, Villemagne B, Herledan A, Hot D, Willand N, Hartkoorn RC
- journal: Appl Microbiol Biotechnol
- DOI: 10.1007/s00253-021-11404-w
- year: 2021
- mesh: *Actinobacteria/genetics, *Biological Products, *Micromonosporaceae, Multigene Family, Pyridines
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 11294 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43823) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43823 | |||
| 19960 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM43823.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68368 | Automatically annotated from API 20E | |||||
| 77310 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40923.1 | StrainInfo: A central database for resolving microbial strain identifiers |