Strain identifier

BacDive ID: 7850

Type strain: Yes

Species: Asanoa iriomotensis

Strain Designation: TT 97-02

Strain history: NBRC 100142 <-- T. Tamura TT 97-02.

NCBI tax ID(s): 234613 (species)

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General

@ref: 12054

BacDive-ID: 7850

DSM-Number: 44745

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Asanoa iriomotensis TT 97-02 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil of mangrove.

NCBI tax id

  • NCBI tax id: 234613
  • Matching level: species

strain history

@refhistory
12054<- T. Tamura; TT 97-02
67770NBRC 100142 <-- T. Tamura TT 97-02.

doi: 10.13145/bacdive7850.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Asanoa
  • species: Asanoa iriomotensis
  • full scientific name: Asanoa iriomotensis Tamura and Sakane 2005

@ref: 12054

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Asanoa

species: Asanoa iriomotensis

full scientific name: Asanoa iriomotensis Tamura and Sakane 2005

strain designation: TT 97-02

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
30136positiveno
69480no95.188
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
20109Yellow orange (2000)10-14 daysISP 2
20109Yellow orange (2000)10-14 daysISP 3
2010910-14 daysISP 4
2010910-14 daysISP 5
2010910-14 daysISP 6
2010910-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
20109noISP 2
20109noISP 3
20109noISP 4
20109noISP 5
20109noISP 6
20109noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12054GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
12054STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
20109ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20109ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20109ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20109ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20109ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20109ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
12054GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
12054TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20109positiveoptimum28mesophilic
12054positivegrowth28mesophilic
30136positivegrowth20-30
30136positiveoptimum25mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 30136
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30136yes
69481yes92
69480yes100

observation

@refobservation
30136aggregates in chains
67770quinones: MK-10(H6), MK-10(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3013629864mannitol+carbon source
3013617306maltose+carbon source
3013617234glucose+carbon source
3013628260galactose+carbon source
3013622599arabinose+carbon source
2010917234glucose+/-
2010922599arabinose+/-
2010917992sucrose+/-
2010918222xylose+/-
2010917268myo-inositol+/-
2010929864mannitol+/-
2010928757fructose+/-
2010926546rhamnose+/-
2010916634raffinose+/-
2010962968cellulose+/-
3013637684mannose+carbon source
3013628053melibiose+carbon source
3013616634raffinose+carbon source
3013626546rhamnose+carbon source
3013618222xylose+carbon source

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20109+++-+----++++-++++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12054soil of mangroveIriomote-island, Okinawa pref.JapanJPNAsia
67770Mangrove soil along the Shiira RiverIriomote Island, Okinawa Pref.JapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_77379.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_404;97_11524;98_18497;99_77379&stattab=map
  • Last taxonomy: Asanoa
  • 16S sequence: AB112081
  • Sequence Identity:
  • Total samples: 44
  • soil counts: 41
  • aquatic counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
120541Risk group (German classification)
201091German classification

Sequence information

16S sequences

  • @ref: 12054
  • description: Asanoa iriomotensis gene for 16S ribosomal RNA, partial sequence
  • accession: AB112081
  • length: 1471
  • database: ena
  • NCBI tax ID: 234613

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Asanoa iriomotensis NBRC 100142GCA_016862515contigncbi234613
66792Asanoa iriomotensis strain NBRC 100142234613.3wgspatric234613

GC content

@refGC-contentmethod
3013669
6777069high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes92no
motileno90.077no
gram-positiveyes88.149yes
anaerobicno98.817yes
aerobicyes92.367yes
halophileno93.885no
spore-formingyes92.067yes
glucose-utilyes88.127yes
flagellatedno98.186no
thermophileno97.108yes
glucose-fermentno90.932no

External links

@ref: 12054

culture collection no.: DSM 44745, NBRC 100142, JCM 13312, BCRC 16825, CGMCC 4.5571, CIP 108714

straininfo link

  • @ref: 77287
  • straininfo: 137996

literature

  • topic: Phylogeny
  • Pubmed-ID: 15774651
  • title: Asanoa iriomotensis sp. nov., isolated from mangrove soil.
  • authors: Tamura T, Sakane T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02982-0
  • year: 2005
  • mesh: DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Japan, Micromonosporaceae/chemistry/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Rhizophoraceae/*microbiology, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12054Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44745)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44745
20109Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44745.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30136Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2648728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77287Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID137996.1StrainInfo: A central database for resolving microbial strain identifiers