Strain identifier

BacDive ID: 7846

Type strain: Yes

Species: Actinoplanes octamycinicus

Strain history: IFO 14524 <-- INA 4041.

NCBI tax ID(s): 135948 (species)

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General

@ref: 18276

BacDive-ID: 7846

DSM-Number: 45809

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, antibiotic compound production

description: Actinoplanes octamycinicus DSM 45809 is a mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 135948
  • Matching level: species

strain history

@refhistory
18276<- KCTC; KCTC 9593 <- IFO <- G. F. Gauze, INA
67770IFO 14524 <-- INA 4041.

doi: 10.13145/bacdive7846.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes octamycinicus
  • full scientific name: Actinoplanes octamycinicus corrig. (ex Gauze et al. 1979) Tamura et al. 2011

@ref: 18276

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes octamycinicus

full scientific name: Actinoplanes octamycinicus (ex Gauze et al. 1979) Tamura et al. 2011

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69442Chocolate brown (8017), Signal brown (8002)suter without tyrosine
69442Chocolate brown (8017), Chesnut brown (8015)ISP 5
69442Chocolate brown (8017), Chestnut brown (8015), Beige brown (8024)ISP 2
69442Grey brown (8019), Chesnut brown (8015)ISP 7
69442Grey brown (8019), Signal black (9004), Nut brown (8011)suter with tyrosine
69442Khaki grey (7008), Chocolate brown (8017)ISP 6
69442Ochre brown (8001), Pastel yellow (1034), Chocolate brown (8017), Chestnut brown (8015)ISP 3
69442Ochre brown (8001), Sepia brown (8014), Saffron yellow (1017), Lemon yellow (1012)ISP 4

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69442yesAerial myceliumBasalt grey (7012), anthracite grey (7016)ISP 2
69442noAerial myceliumISP 3
69442noAerial myceliumISP 4
69442yesAerial myceliumTelegrey 1 (7045), basalt grey (7012)ISP 5
69442noAerial myceliumISP 6
69442yesAerial myceliumBlack red (3007)ISP 7
69442yesAerial myceliumMouse grey (7005)suter with tyrosine
69442noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69442noMelanin
69442yessoluble pigmentBrown beige (1011), lemon yellow (1012), purple red (3004), pale brown (8025), ochre yellow (1024), ochre brown (8001), oxide red (3009)

multimedia

@refmultimedia contentcaptionintellectual property rights
18276https://www.dsmz.de/microorganisms/photos/DSM_45809.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69442DSM_45809_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69442DSM_45809_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18276GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18276GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf

culture temp

@refgrowthtypetemperaturerange
18276positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

compound production

@refcompound
67770octamycin
67770Purpuromycin

halophily

  • @ref: 69442
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6944222599arabinose+growth
6944262968cellulose+growth
6944228757fructose+growth
6944217234glucose+growth
6944217268inositol-growth
6944237684mannose+growth
6944216634raffinose+/-growth
6944226546rhamnose+growth
6944217992sucrose+growth
6944218222xylose+growth
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69442+--+-++-+-+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69442+++/--+++/-+++++/-+-+++--

Safety information

risk assessment

  • @ref: 18276
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18276
  • description: Actinoplanes octamycinicus gene for 16S rRNA, partial sequence
  • accession: AB047494
  • length: 1470
  • database: ena
  • NCBI tax ID: 135948

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinoplanes octamycinicus NBRC 14524GCA_016862295contigncbi135948
66792Actinoplanes octamycinicus DSM 45809GCA_014205225contigncbi135948
66792Actinoplanes octamycinicus strain DSM 45809135948.4wgspatric135948
66792Actinoplanes octamycinicus strain NBRC 14524135948.5wgspatric135948
66792Actinoplanes octamycinicus DSM 458092872757966draftimg135948

GC content

  • @ref: 18276
  • GC-content: 72.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes59.779no
gram-positiveyes87.502no
anaerobicno98.861no
halophileno96.124no
spore-formingyes91.649no
thermophileno98.974yes
glucose-utilyes90.616yes
flagellatedno89.18no
aerobicyes93.376no
glucose-fermentno89.523yes

External links

@ref: 18276

culture collection no.: DSM 45809, ATCC 43632, INA 4041, JCM 9649, KCTC 9593, NBRC 14524, BCC 49181, IFO 14524, TBRC 173

straininfo link

  • @ref: 77283
  • straininfo: 41951

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21257697Descriptions of Actinoplanes ianthinogenes nom. rev. and Actinoplanes octamycinicus corrig. comb. nov., nom. rev.Tamura T, Ishida Y, Suzuki KIInt J Syst Evol Microbiol10.1099/ijs.0.030130-02011Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Micromonosporaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny23396720Actinoplanes siamensis sp. nov., isolated from soil.Suriyachadkun C, Ngaemthao W, Chunhametha S, Thawai C, Sanglier JJInt J Syst Evol Microbiol10.1099/ijs.0.048017-02013Bacterial Typing Techniques, Bambusa/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/analysisGenetics

Reference

@idauthorscataloguedoi/urltitle
18276Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45809)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45809
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69442Wink, J.https://cdn.dsmz.de/wink/DSM%2045809.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
77283Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41951.1StrainInfo: A central database for resolving microbial strain identifiers