Strain identifier

BacDive ID: 7808

Type strain: Yes

Species: Actinoplanes lobatus

Strain Designation: UNCC 72

Culture col. no.: DSM 43150, ATCC 15350, CBS 192.64, IFO 12513, KCC A-0061, NBRC 12513

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General

@ref: 10820

BacDive-ID: 7808

DSM-Number: 43150

keywords: spore-forming, Bacteria, 16S sequence, genome sequence, mesophilic

description: Actinoplanes lobatus UNCC 72 is a spore-forming, mesophilic bacterium that was isolated from soil.

strain history: <- KCC <- CBS <- J.N. Couch, UNCC, 72

doi: 10.13145/bacdive7808.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes lobatus
  • full scientific name: Actinoplanes lobatus (Couch 1963) Stackebrandt and Kroppenstedt 1988
  • synonyms

    @refsynonym
    20215Ampullaria lobata
    20215Ampullariella lobata

@ref: 10820

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes lobatus

full scientific name: Actinoplanes lobatus (Couch 1963) Stackebrandt and Kroppenstedt 1988

strain designation: UNCC 72

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19548Yellow orange10-14 daysISP 2
19548Yellow orange10-14 daysISP 3
19548Yellow orange10-14 daysISP 4
19548Yellow orange10-14 daysISP 5
19548Yellow orange10-14 daysISP 6
19548Yellow orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19548noISP 2
19548noISP 3
19548noISP 4
19548noISP 5
19548noISP 6
19548noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
19548ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19548ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19548ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19548ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19548ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19548ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10820CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium83.pdf

culture temp

@refgrowthtypetemperaturerange
19548positiveoptimum28mesophilic
10820positivegrowth37mesophilic

Physiology and metabolism

spore formation

  • @ref: 19548
  • spore description: Spore surface: smooth, sporangia: +
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19548
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19548-----------

Isolation, sampling and environmental information

isolation

  • @ref: 10820
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
195481Risk group (German classification)
108201Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinoplanes lobatus gene for 16S rRNA, partial sequence
  • accession: AB037006
  • length: 1472
  • database: ena
  • NCBI tax ID: 113568

Genome sequences

  • @ref: 66792
  • description: Actinoplanes lobatus DSM 43150
  • accession: 2873296955
  • assembly level: permanent
  • database: img
  • NCBI tax ID: 113568

External links

@ref: 10820

culture collection no.: DSM 43150, ATCC 15350, CBS 192.64, IFO 12513, KCC A-0061, NBRC 12513

straininfo link

@refpassport
20218http://www.straininfo.net/strains/98321
20218http://www.straininfo.net/strains/98319
20218http://www.straininfo.net/strains/228989
20218http://www.straininfo.net/strains/98322
20218http://www.straininfo.net/strains/167917
20218http://www.straininfo.net/strains/333326

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10820Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43150)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43150
19548Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43150.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)