Strain identifier

BacDive ID: 7802

Type strain: Yes

Species: Actinoplanes deccanensis

Strain Designation: A/10655, MB-T 21

Strain history: KCC A-0247 <-- SANK 61978 <-- ATCC 21983 <-- Lepetit A/10655.

NCBI tax ID(s): 113561 (species)

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General

@ref: 11259

BacDive-ID: 7802

DSM-Number: 43806

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, antibiotic compound production

description: Actinoplanes deccanensis A/10655 is a spore-forming, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from Soil.

NCBI tax id

  • NCBI tax id: 113561
  • Matching level: species

strain history

@refhistory
11259<- G. Vobis, MB-T 21 <- Gruppo Lepetit S.p.A., A/10655 (Actinoplanes sp.)
67770KCC A-0247 <-- SANK 61978 <-- ATCC 21983 <-- Lepetit A/10655.

doi: 10.13145/bacdive7802.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes deccanensis
  • full scientific name: Actinoplanes deccanensis Parenti et al. 1975 (Approved Lists 1980)

@ref: 11259

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes deccanensis

full scientific name: Actinoplanes deccanensis Parenti et al. 1975

strain designation: A/10655, MB-T 21

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium used
19591Dahlia yellow10-14 daysISP 2
19591Pastel yellow10-14 daysISP 3
19591Pastel yellow10-14 daysISP 4
19591Pastel yellow10-14 daysISP 5
19591Pastel yellow10-14 daysISP 6
19591Pastel yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19591noISP 2
19591noISP 3
19591noISP 4
19591noISP 5
19591noISP 6
19591noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
11259https://www.dsmz.de/microorganisms/photos/DSM_43806-1.jpg© Leibniz-Institut DSMZ
11259https://www.dsmz.de/microorganisms/photos/DSM_43806.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11259GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
19591ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19591ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19591ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19591ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19591ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19591ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11259GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19591positiveoptimum28mesophilic
11259positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore descriptiontype of sporespore formationconfidence
19591Formation of sporangia, spore surface smoothsporeyes
69481yes100
69480yes100

compound production

@refcompound
11259lipiarmycin
20216Lipiarmycin
19591Lipiarmycin A
19591Azaserine

halophily

  • @ref: 19591
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777068590fidaxomicinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19591+++-+--+++++++++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19591----+----++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil
  • geographic location: Decca
  • country: India
  • origin.country: IND
  • continent: Asia

taxonmaps

  • @ref: 69479
  • File name: preview.99_57520.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_404;97_1769;98_40991;99_57520&stattab=map
  • Last taxonomy: Actinoplanes
  • 16S sequence: AB036998
  • Sequence Identity:
  • Total samples: 46
  • soil counts: 33
  • aquatic counts: 4
  • animal counts: 3
  • plant counts: 6

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112591Risk group (German classification)
195911Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinoplanes deccanensis gene for 16S rRNA, partial sequenceAB0369981477ena113561
20218Actinoplanes deccanensis partial 16S rRNA gene, strain IMSNU 20026TAJ2775651452ena113561

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinoplanes deccanensis NBRC 13994GCA_016862115contigncbi113561
66792Actinoplanes deccanensis strain NBRC 13994113561.33wgspatric113561

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes65.659no
flagellatedno89.711no
gram-positiveyes86.771no
anaerobicno99.085no
aerobicyes91.308no
halophileno96.276no
spore-formingyes91.576yes
thermophileno99.042yes
glucose-utilyes91.683no
glucose-fermentno90.087no

External links

@ref: 11259

culture collection no.: DSM 43806, ATCC 21983, JCM 3247, KCC A-0247, BCRC 12107, CBS 597.74, CGMCC 4.2098, IFO 13994, IMET 9256, IMSNU 20026, KCTC 9532, MTCC 711, NBRC 13994, NCIMB 12643, NRRL B-16715

straininfo link

  • @ref: 77244
  • straininfo: 88612

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3366689Structure and biological activity of lipiarmycin B.Cavalleri B, Arnone A, Di Modugno E, Nasini G, Goldstein BPJ Antibiot (Tokyo)10.7164/antibiotics.41.3081988*Aminoglycosides, Animals, *Anti-Bacterial Agents/isolation & purification/pharmacology, Chemical Phenomena, Chemistry, Fidaxomicin, Magnetic Resonance Spectroscopy, Mice, Microbial Sensitivity TestsPhylogeny
Phylogeny21669926Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil.Sazak A, Sahin N, Camas MInt J Syst Evol Microbiol10.1099/ijs.0.031872-02011Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nigeria, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny22180611Actinoplanes atraurantiacus sp. nov., isolated from soil.Zhang Y, Zhang J, Fan L, Pang H, Xin Y, Zhang XInt J Syst Evol Microbiol10.1099/ijs.0.037226-02011Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, Sequence Analysis, DNA, Soil/analysis, *Soil Microbiology, Vitamin K 2/analysisGenetics
Phylogeny28905706Classification of Actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as Actinoplanes ramoplaninifer sp. nov.Marcone GL, Binda E, Reguzzoni M, Gastaldo L, Dalmastri C, Marinelli FInt J Syst Evol Microbiol10.1099/ijsem.0.0022812017Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Depsipeptides/*biosynthesis, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29125458Actinoplanes sediminis sp. nov., isolated from marine sediment.Qu Z, Bao XD, Xie QY, Zhao YX, Yan B, Dai HF, Chen HQInt J Syst Evol Microbiol10.1099/ijsem.0.0024512017Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Geologic Sediments, Greece, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11259Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43806)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43806
19591Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43806.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77244Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88612.1StrainInfo: A central database for resolving microbial strain identifiers