Strain identifier

BacDive ID: 7797

Type strain: Yes

Species: Actinocatenispora rupis

Strain Designation: CS5-AC17

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15642

BacDive-ID: 7797

DSM-Number: 45178

keywords: mesophilic, spore-forming, aerobe, Bacteria, 16S sequence, gram-positive

description: Actinocatenispora rupis CS5-AC17 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil of cliff.

strain history: <- S. D. Lee; CS5-AC17

doi: 10.13145/bacdive7797.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinocatenispora
  • species: Actinocatenispora rupis
  • full scientific name: Actinocatenispora rupis Seo and Lee 2009

@ref: 15642

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinocatenispora

species: Actinocatenispora rupis

full scientific name: Actinocatenispora rupis Seo and Lee 2009

strain designation: CS5-AC17

type strain: yes

Morphology

cell morphology

  • @ref: 29428
  • gram stain: positive
  • cell length: 1.25 µm
  • cell width: 0.45 µm

colony morphology

@refcolony colorincubation periodmedium used
20175Light ivory (1015)10-14 daysISP 2
20175Cream (9001)10-14 daysISP 3
20175Light ivory (1015)10-14 daysISP 4
20175Light ivory (1015)10-14 daysISP 5
20175Ivory (1014)10-14 daysISP 6
20175Cream (9001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
20175noISP 2
20175noISP 3
20175noISP 4
20175noISP 5
20175noISP 6
20175noISP 7

multimedia

  • @ref: 15642
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45178.jpg
  • caption: Medium 65 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

pigmentation

  • @ref: 29428
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15642ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
15642GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
20175ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20175ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20175ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20175ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20175ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20175ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15642positivegrowth28mesophilic
20175positiveoptimum28mesophilic
29428positivegrowth25-42
29428positiveoptimum37mesophilic

culture pH

@refabilitytypepH
29428positivegrowth5.1-12.1
29428positiveoptimum7.1

Physiology and metabolism

oxygen tolerance

  • @ref: 29428
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29428
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29428NaClpositivegrowth0-4 %
29428NaClpositiveoptimum7.1 %

observation

  • @ref: 29428
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2017517234glucose+
2017522599arabinose+/-
2017517992sucrose+
2017518222xylose+/-
2017517268myo-inositol+/-
2017529864mannitol+
2017528757fructose+
2017526546rhamnose+/-
2017516634raffinose-
2017562968cellulose-
2942815963ribitol+carbon source
2942822599arabinose+carbon source
2942817057cellobiose+carbon source
2942816947citrate+carbon source
2942828260galactose+carbon source
2942817754glycerol+carbon source
2942817716lactose+carbon source
2942817306maltose+carbon source
2942829864mannitol+carbon source
2942837684mannose+carbon source
2942826546rhamnose+carbon source
2942830911sorbitol+carbon source
2942817992sucrose+carbon source
2942827082trehalose+carbon source
2942853426tween 80+carbon source
294284853esculin+hydrolysis

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20175+++-++-+-++++++++++

Isolation, sampling and environmental information

isolation

  • @ref: 15642
  • sample type: soil of cliff
  • geographic location: Jeju, Mara Island
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Geologic
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 15642
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15642
  • description: Actinocatenispora rupis partial 16S rRNA gene, type strain CS5-AC17T
  • accession: AM980986
  • length: 1408
  • database: ena
  • NCBI tax ID: 519421

GC content

@refGC-contentmethod
1564274.3high performance liquid chromatography (HPLC)
2942874.3

External links

@ref: 15642

culture collection no.: DSM 45178, JCM 16894, NBRC 107355, NRRL B-24660

straininfo link

@refpassport
20218http://www.straininfo.net/strains/846002
20218http://www.straininfo.net/strains/856358
20218http://www.straininfo.net/strains/846003

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
15642Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45178)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45178
20175Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45178.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2583410.1099/ijs.0.012922-0
29428Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information25834