Strain identifier
BacDive ID: 7785
Type strain:
Species: Zhihengliuella alba
Strain history: <- SK Tang, Yunnan Inst., China
NCBI tax ID(s): 547018 (species)
General
@ref: 15489
BacDive-ID: 7785
DSM-Number: 21143
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped
description: Zhihengliuella alba DSM 21143 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from saline soil.
NCBI tax id
- NCBI tax id: 547018
- Matching level: species
strain history
@ref | history |
---|---|
15489 | <- S.-K. Tang, Yunnan Univ., Kunming, Yunnan Inst. Microbiol. (YIM), China |
67770 | KCTC 19375 <-- S.-K. Tang YIM 90734. |
67771 | <- SK Tang, Yunnan Inst., China |
doi: 10.13145/bacdive7785.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Micrococcaceae
- genus: Zhihengliuella
- species: Zhihengliuella alba
- full scientific name: Zhihengliuella alba Tang et al. 2009
@ref: 15489
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Micrococcaceae
genus: Zhihengliuella
species: Zhihengliuella alba
full scientific name: Zhihengliuella alba Tang et al. 2009
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
29148 | positive | 1.55 µm | 0.7 µm | rod-shaped | no |
67771 | positive |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19792 | Sand yellow | 10-14 days | ISP 2 |
19792 | Ivory | 10-14 days | ISP 3 |
19792 | Ivory | 10-14 days | ISP 4 |
19792 | Ivory | 10-14 days | ISP 5 |
19792 | Ivory | 10-14 days | ISP 6 |
19792 | Ivory | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19792 | no | ISP 2 |
19792 | no | ISP 3 |
19792 | no | ISP 4 |
19792 | no | ISP 5 |
19792 | no | ISP 6 |
19792 | no | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15489 | RICH MEDIUM (DSMZ Medium 736) | yes | https://mediadive.dsmz.de/medium/736 | Name: RICH MEDIUM (DSMZ Medium 736) Composition: Agar 20.0 g/l Bacto peptone 10.0 g/l Yeast extract 5.0 g/l Malt extract 5.0 g/l Casamino acids 5.0 g/l Glycerol 2.0 g/l Meat extract 2.0 g/l MgSO4 x 7 H2O 1.0 g/l Tween 80 0.05 g/l Distilled water |
19792 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19792 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19792 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19792 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19792 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19792 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15489 | positive | growth | 28 | mesophilic |
19792 | positive | optimum | 28 | mesophilic |
29148 | positive | growth | 4.0-45 | |
29148 | positive | optimum | 32.5 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
67771 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
29148 | positive | growth | 5.0-9.0 | alkaliphile |
29148 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
29148 | aerobe |
67771 | aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29148 | NaCl | positive | growth | 0.0-15 % |
29148 | NaCl | positive | optimum | 5 % |
murein
- @ref: 15489
- murein short key: A11.35
- type: A4alpha L-Lys-L-Ala-L-Glu
observation
- @ref: 67770
- observation: quinones: MK-10, MK-9
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29148 | 28644 | 2-oxopentanoate | + | carbon source |
29148 | 18403 | L-arabitol | + | carbon source |
29148 | 22653 | asparagine | + | carbon source |
29148 | 17057 | cellobiose | + | carbon source |
29148 | 23652 | dextrin | + | carbon source |
29148 | 28757 | fructose | + | carbon source |
29148 | 28260 | galactose | + | carbon source |
29148 | 17234 | glucose | + | carbon source |
29148 | 17754 | glycerol | + | carbon source |
29148 | 17716 | lactose | + | carbon source |
29148 | 17306 | maltose | + | carbon source |
29148 | 29864 | mannitol | + | carbon source |
29148 | 37684 | mannose | + | carbon source |
29148 | 17272 | propionate | + | carbon source |
29148 | 15361 | pyruvate | + | carbon source |
29148 | 16634 | raffinose | + | carbon source |
29148 | 26546 | rhamnose | + | carbon source |
29148 | 33942 | ribose | + | carbon source |
29148 | 17822 | serine | + | carbon source |
29148 | 30031 | succinate | + | carbon source |
29148 | 17992 | sucrose | + | carbon source |
29148 | 17748 | thymidine | + | carbon source |
29148 | 27082 | trehalose | + | carbon source |
29148 | 53423 | tween 40 | + | carbon source |
29148 | 53426 | tween 80 | + | carbon source |
29148 | 17151 | xylitol | + | carbon source |
29148 | 18222 | xylose | + | carbon source |
29148 | 4853 | esculin | + | hydrolysis |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29148 | acid phosphatase | + | 3.1.3.2 |
29148 | catalase | + | 1.11.1.6 |
29148 | gelatinase | + | |
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19792 | - | - | + | - | - | + | + | - | - | - | + | - | - | +/- | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19792 | - | + | + | - | + | + | - | - | - | - | + | - | - | - | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15489 | saline soil | Xinjiang province, Ganjiahu Suosuo Forest Natl. Nature Reserve | China | CHN | Asia |
67770 | Saline soil from Ganjiahu Suosuo Forest National Nature Reserve | Xinjiang Province, north-west China | China | CHN | Asia |
67771 | From saline-alkali soil | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Condition | #Saline |
Safety information
risk assessment
- @ref: 15489
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15489
- description: Zhihengliuella alba strain YIM 90734 16S ribosomal RNA gene, partial sequence
- accession: EU847536
- length: 1454
- database: ena
- NCBI tax ID: 547018
GC content
@ref | GC-content | method |
---|---|---|
15489 | 70.3 | high performance liquid chromatography (HPLC) |
29148 | 70.3 |
External links
@ref: 15489
culture collection no.: DSM 21143, KCTC 19375, YIM 90734, JCM 16961, NBRC 109061
straininfo link
- @ref: 77228
- straininfo: 75690
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19567565 | Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. | Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.007344-0 | 2009 | Amino Acids/analysis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Mannose/analysis, Micrococcaceae/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Pigments, Biological/biosynthesis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysis | Genetics |
Phylogeny | 20490581 | Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine. | Chen YG, Tang SK, Zhang YQ, Liu ZX, Chen QH, He JW, Cui XL, Li WJ | Extremophiles | 10.1007/s00792-010-0317-4 | 2010 | Gram-Positive Bacteria/classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Seawater, *Water Microbiology | Genetics |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
15489 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21143) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21143 | |||
19792 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM21143.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29148 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25572 | 28776041 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
77228 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID75690.1 | StrainInfo: A central database for resolving microbial strain identifiers |