Strain identifier

BacDive ID: 7785

Type strain: Yes

Species: Zhihengliuella alba

Strain history: <- SK Tang, Yunnan Inst., China

NCBI tax ID(s): 547018 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 15489

BacDive-ID: 7785

DSM-Number: 21143

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Zhihengliuella alba DSM 21143 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 547018
  • Matching level: species

strain history

@refhistory
15489<- S.-K. Tang, Yunnan Univ., Kunming, Yunnan Inst. Microbiol. (YIM), China
67770KCTC 19375 <-- S.-K. Tang YIM 90734.
67771<- SK Tang, Yunnan Inst., China

doi: 10.13145/bacdive7785.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Zhihengliuella
  • species: Zhihengliuella alba
  • full scientific name: Zhihengliuella alba Tang et al. 2009

@ref: 15489

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Zhihengliuella

species: Zhihengliuella alba

full scientific name: Zhihengliuella alba Tang et al. 2009

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
29148positive1.55 µm0.7 µmrod-shapedno
67771positive

colony morphology

@refcolony colorincubation periodmedium used
19792Sand yellow10-14 daysISP 2
19792Ivory10-14 daysISP 3
19792Ivory10-14 daysISP 4
19792Ivory10-14 daysISP 5
19792Ivory10-14 daysISP 6
19792Ivory10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19792noISP 2
19792noISP 3
19792noISP 4
19792noISP 5
19792noISP 6
19792noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15489RICH MEDIUM (DSMZ Medium 736)yeshttps://mediadive.dsmz.de/medium/736Name: RICH MEDIUM (DSMZ Medium 736) Composition: Agar 20.0 g/l Bacto peptone 10.0 g/l Yeast extract 5.0 g/l Malt extract 5.0 g/l Casamino acids 5.0 g/l Glycerol 2.0 g/l Meat extract 2.0 g/l MgSO4 x 7 H2O 1.0 g/l Tween 80 0.05 g/l Distilled water
19792ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19792ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19792ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19792ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19792ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19792ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15489positivegrowth28mesophilic
19792positiveoptimum28mesophilic
29148positivegrowth4.0-45
29148positiveoptimum32.5mesophilic
67770positivegrowth28mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29148positivegrowth5.0-9.0alkaliphile
29148positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29148aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
29148NaClpositivegrowth0.0-15 %
29148NaClpositiveoptimum5 %

murein

  • @ref: 15489
  • murein short key: A11.35
  • type: A4alpha L-Lys-L-Ala-L-Glu

observation

  • @ref: 67770
  • observation: quinones: MK-10, MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
29148286442-oxopentanoate+carbon source
2914818403L-arabitol+carbon source
2914822653asparagine+carbon source
2914817057cellobiose+carbon source
2914823652dextrin+carbon source
2914828757fructose+carbon source
2914828260galactose+carbon source
2914817234glucose+carbon source
2914817754glycerol+carbon source
2914817716lactose+carbon source
2914817306maltose+carbon source
2914829864mannitol+carbon source
2914837684mannose+carbon source
2914817272propionate+carbon source
2914815361pyruvate+carbon source
2914816634raffinose+carbon source
2914826546rhamnose+carbon source
2914833942ribose+carbon source
2914817822serine+carbon source
2914830031succinate+carbon source
2914817992sucrose+carbon source
2914817748thymidine+carbon source
2914827082trehalose+carbon source
2914853423tween 40+carbon source
2914853426tween 80+carbon source
2914817151xylitol+carbon source
2914818222xylose+carbon source
291484853esculin+hydrolysis
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
29148acid phosphatase+3.1.3.2
29148catalase+1.11.1.6
29148gelatinase+
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19792--+--++---+--+/------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19792-++-++----+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15489saline soilXinjiang province, Ganjiahu Suosuo Forest Natl. Nature ReserveChinaCHNAsia
67770Saline soil from Ganjiahu Suosuo Forest National Nature ReserveXinjiang Province, north-west ChinaChinaCHNAsia
67771From saline-alkali soilChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

Safety information

risk assessment

  • @ref: 15489
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15489
  • description: Zhihengliuella alba strain YIM 90734 16S ribosomal RNA gene, partial sequence
  • accession: EU847536
  • length: 1454
  • database: ena
  • NCBI tax ID: 547018

GC content

@refGC-contentmethod
1548970.3high performance liquid chromatography (HPLC)
2914870.3

External links

@ref: 15489

culture collection no.: DSM 21143, KCTC 19375, YIM 90734, JCM 16961, NBRC 109061

straininfo link

  • @ref: 77228
  • straininfo: 75690

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19567565Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella.Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.007344-02009Amino Acids/analysis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Mannose/analysis, Micrococcaceae/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Pigments, Biological/biosynthesis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysisGenetics
Phylogeny20490581Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine.Chen YG, Tang SK, Zhang YQ, Liu ZX, Chen QH, He JW, Cui XL, Li WJExtremophiles10.1007/s00792-010-0317-42010Gram-Positive Bacteria/classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Seawater, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15489Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21143)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21143
19792Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM21143.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29148Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2557228776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
77228Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID75690.1StrainInfo: A central database for resolving microbial strain identifiers