Strain identifier

BacDive ID: 7784

Type strain: Yes

Species: Zhihengliuella halotolerans

Strain history: <- WJ Li, Yunnan Institute of Microbiology, China

NCBI tax ID(s): 370736 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6834

BacDive-ID: 7784

DSM-Number: 17364

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Zhihengliuella halotolerans DSM 17364 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 370736
  • Matching level: species

strain history

@refhistory
6834<- W.-J. Li; YIM
67770KCTC 19085 <-- W.-J. Li YIM 70185.
67771<- WJ Li, Yunnan Institute of Microbiology, China

doi: 10.13145/bacdive7784.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Zhihengliuella
  • species: Zhihengliuella halotolerans
  • full scientific name: Zhihengliuella halotolerans Zhang et al. 2007

@ref: 6834

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Zhihengliuella

species: Zhihengliuella halotolerans

full scientific name: Zhihengliuella halotolerans Zhang et al. 2007

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
31851positiverod-shapedno
67771positive
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
19747Zinc yellow (1018)10-14 daysISP 3
19747Cream (9001)10-14 daysISP 5
19747Cream (9001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19747noISP 3
19747noISP 5
19747noISP 7

pigmentation

  • @ref: 31851
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6834CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://mediadive.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
19747ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19747ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19747ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
6834positivegrowth28mesophilic
19747positiveoptimum28mesophilic
31851positiveoptimum29mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

  • @ref: 31851
  • ability: positive
  • type: optimum
  • pH: 8.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31851aerobe
67771aerobe

spore formation

@refspore formationconfidence
31851no
69481no100
69480no99.997

halophily

  • @ref: 31851
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 10 %

murein

  • @ref: 6834
  • murein short key: A11.35
  • type: A4alpha L-Lys-L-Ala-L-Glu

observation

@refobservation
67770quinones: MK-9, MK-10, MK-8
67771quinones: MK-9, MK-10, MK-8(molar ratio 5:2:1)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3185122599arabinose+carbon source
3185117234glucose+carbon source
3185117306maltose+carbon source
3185117992sucrose+carbon source
3185127082trehalose+carbon source
3185153424tween 20+carbon source
3185153426tween 80+carbon source
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
31851alpha-galactosidase+3.2.1.22
31851catalase+1.11.1.6
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19747-++--++-+++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19747-+++++----++++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6834saline soilQinghai ProvinceChinaCHNAsia
67770Saline soil from Qinghai ProvinceChinaCHNAsia
67771From contaminated plateQinghai ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_60043.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_158;97_13038;98_16043;99_60043&stattab=map
  • Last taxonomy: Zhihengliuella halotolerans
  • 16S sequence: DQ372937
  • Sequence Identity:
  • Total samples: 6
  • soil counts: 2
  • animal counts: 4

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68341Risk group (German classification)
197471Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6834
  • description: Zhihengliuella halotolerans strain YIM 70185 16S ribosomal RNA gene, partial sequence
  • accession: DQ372937
  • length: 1475
  • database: ena
  • NCBI tax ID: 370736

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Zhihengliuella halotolerans strain DSM 17364370736.4wgspatric370736
66792Zhihengliuella halotolerans DSM 173642806310507draftimg370736
67770Zhihengliuella halotolerans DSM 17364GCA_004217565contigncbi370736

GC content

@refGC-contentmethod
683466.5
6777066.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveyes94.394yes
anaerobicno99.547yes
halophileyes60.965yes
spore-formingno89.997yes
glucose-utilyes91.281yes
aerobicyes94.54no
flagellatedno95.558no
thermophileno99.084no
motileno91.907yes
glucose-fermentno80.273no

External links

@ref: 6834

culture collection no.: DSM 17364, KCTC 19085, YIM 70185, JCM 15629, NBRC 107838

straininfo link

  • @ref: 77227
  • straininfo: 405455

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17473251Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae.Zhang YQ, Schumann P, Yu LY, Liu HY, Zhang YQ, Xu LH, Stackebrandt E, Jiang CL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.64528-02007Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Micrococcaceae/chemistry/*classification/genetics/*isolation & purification, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Vitamin K 2/analysisGenetics
Phylogeny20490581Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine.Chen YG, Tang SK, Zhang YQ, Liu ZX, Chen QH, He JW, Cui XL, Li WJExtremophiles10.1007/s00792-010-0317-42010Gram-Positive Bacteria/classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Seawater, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
6834Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17364)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17364
19747Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM17364.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31851Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2811528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77227Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405455.1StrainInfo: A central database for resolving microbial strain identifiers