Strain identifier
BacDive ID: 7784
Type strain:
Species: Zhihengliuella halotolerans
Strain history: <- WJ Li, Yunnan Institute of Microbiology, China
NCBI tax ID(s): 370736 (species)
General
@ref: 6834
BacDive-ID: 7784
DSM-Number: 17364
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped
description: Zhihengliuella halotolerans DSM 17364 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from saline soil.
NCBI tax id
- NCBI tax id: 370736
- Matching level: species
strain history
@ref | history |
---|---|
6834 | <- W.-J. Li; YIM |
67770 | KCTC 19085 <-- W.-J. Li YIM 70185. |
67771 | <- WJ Li, Yunnan Institute of Microbiology, China |
doi: 10.13145/bacdive7784.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Micrococcaceae
- genus: Zhihengliuella
- species: Zhihengliuella halotolerans
- full scientific name: Zhihengliuella halotolerans Zhang et al. 2007
@ref: 6834
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Micrococcaceae
genus: Zhihengliuella
species: Zhihengliuella halotolerans
full scientific name: Zhihengliuella halotolerans Zhang et al. 2007
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | confidence |
---|---|---|---|---|
31851 | positive | rod-shaped | no | |
67771 | positive | |||
69480 | positive | 100 |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19747 | Zinc yellow (1018) | 10-14 days | ISP 3 |
19747 | Cream (9001) | 10-14 days | ISP 5 |
19747 | Cream (9001) | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19747 | no | ISP 3 |
19747 | no | ISP 5 |
19747 | no | ISP 7 |
pigmentation
- @ref: 31851
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6834 | CORYNEBACTERIUM AGAR (DSMZ Medium 53) | yes | https://mediadive.dsmz.de/medium/53 | Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water |
19747 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19747 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19747 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6834 | positive | growth | 28 | mesophilic |
19747 | positive | optimum | 28 | mesophilic |
31851 | positive | optimum | 29 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
67771 | positive | growth | 28 | mesophilic |
culture pH
- @ref: 31851
- ability: positive
- type: optimum
- pH: 8.5
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
31851 | aerobe |
67771 | aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
31851 | no | |
69481 | no | 100 |
69480 | no | 99.997 |
halophily
- @ref: 31851
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 10 %
murein
- @ref: 6834
- murein short key: A11.35
- type: A4alpha L-Lys-L-Ala-L-Glu
observation
@ref | observation |
---|---|
67770 | quinones: MK-9, MK-10, MK-8 |
67771 | quinones: MK-9, MK-10, MK-8(molar ratio 5:2:1) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31851 | 22599 | arabinose | + | carbon source |
31851 | 17234 | glucose | + | carbon source |
31851 | 17306 | maltose | + | carbon source |
31851 | 17992 | sucrose | + | carbon source |
31851 | 27082 | trehalose | + | carbon source |
31851 | 53424 | tween 20 | + | carbon source |
31851 | 53426 | tween 80 | + | carbon source |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
31851 | alpha-galactosidase | + | 3.2.1.22 |
31851 | catalase | + | 1.11.1.6 |
68379 | gelatinase | + | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | + | 3.5.1.B15 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | beta-glucuronidase | + | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19747 | - | + | + | - | - | + | + | - | + | + | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19747 | - | + | + | + | + | + | - | - | - | - | + | + | + | + | + | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
6834 | saline soil | Qinghai Province | China | CHN | Asia |
67770 | Saline soil from Qinghai Province | China | CHN | Asia | |
67771 | From contaminated plate | Qinghai Province | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_60043.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_158;97_13038;98_16043;99_60043&stattab=map
- Last taxonomy: Zhihengliuella halotolerans
- 16S sequence: DQ372937
- Sequence Identity:
- Total samples: 6
- soil counts: 2
- animal counts: 4
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6834 | 1 | Risk group (German classification) |
19747 | 1 | Risk group (German classification) |
Sequence information
16S sequences
- @ref: 6834
- description: Zhihengliuella halotolerans strain YIM 70185 16S ribosomal RNA gene, partial sequence
- accession: DQ372937
- length: 1475
- database: ena
- NCBI tax ID: 370736
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Zhihengliuella halotolerans strain DSM 17364 | 370736.4 | wgs | patric | 370736 |
66792 | Zhihengliuella halotolerans DSM 17364 | 2806310507 | draft | img | 370736 |
67770 | Zhihengliuella halotolerans DSM 17364 | GCA_004217565 | contig | ncbi | 370736 |
GC content
@ref | GC-content | method |
---|---|---|
6834 | 66.5 | |
67770 | 66.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | yes | 94.394 | yes |
anaerobic | no | 99.547 | yes |
halophile | yes | 60.965 | yes |
spore-forming | no | 89.997 | yes |
glucose-util | yes | 91.281 | yes |
aerobic | yes | 94.54 | no |
flagellated | no | 95.558 | no |
thermophile | no | 99.084 | no |
motile | no | 91.907 | yes |
glucose-ferment | no | 80.273 | no |
External links
@ref: 6834
culture collection no.: DSM 17364, KCTC 19085, YIM 70185, JCM 15629, NBRC 107838
straininfo link
- @ref: 77227
- straininfo: 405455
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17473251 | Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae. | Zhang YQ, Schumann P, Yu LY, Liu HY, Zhang YQ, Xu LH, Stackebrandt E, Jiang CL, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.64528-0 | 2007 | Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Micrococcaceae/chemistry/*classification/genetics/*isolation & purification, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil Microbiology, Vitamin K 2/analysis | Genetics |
Phylogeny | 20490581 | Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine. | Chen YG, Tang SK, Zhang YQ, Liu ZX, Chen QH, He JW, Cui XL, Li WJ | Extremophiles | 10.1007/s00792-010-0317-4 | 2010 | Gram-Positive Bacteria/classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Seawater, *Water Microbiology | Genetics |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
6834 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17364) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17364 | ||||
19747 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM17364.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
31851 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28115 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||||
68379 | Automatically annotated from API Coryne | ||||||
68382 | Automatically annotated from API zym | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
77227 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405455.1 | StrainInfo: A central database for resolving microbial strain identifiers |