Strain identifier

BacDive ID: 7641

Type strain: Yes

Species: Kocuria rosea

Strain history: CCM 679 <-- ATCC 186 <-- R. S. Breed ("Micrococcus rubens") <-- Král Collection <-- W. Migula <-- O. Bujwid.

NCBI tax ID(s): 1275 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8819

BacDive-ID: 7641

DSM-Number: 20447

keywords: mesophilic, aerobe, Bacteria, 16S sequence, genome sequence

description: Kocuria rosea DSM 20447 is an aerobe, mesophilic bacterium of the family Micrococcaceae.

NCBI tax id

  • NCBI tax id: 1275
  • Matching level: species

strain history

doi: 10.13145/bacdive7641.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Kocuria
  • species: Kocuria rosea
  • full scientific name: Kocuria rosea (Flügge 1886) Stackebrandt et al. 1995
  • synonyms

    @refsynonym
    20215Kocuria erythromyxa
    20215Micrococcus roseus
    20215Deinococcus erythromyxa
    20215Pelczaria aurantia

@ref: 8819

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Kocuria

species: Kocuria rosea

full scientific name: Kocuria rosea (Flügge 1886) Stackebrandt et al. 1995

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18746Salmon orange10-14 days5006
18746Salmon orange10-14 days5425
18746Salmon orange10-14 days5428
18746Salmon orange10-14 days5530
18746Salmon orange10-14 daysISP 2

multicellular morphology

@refforms multicellular complexmedium name
18746noISP 2
18746no5006
18746no5425
18746no5530

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8819CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://bacmedia.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18746ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
187465006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
187465425yesName: Brain Heart Infusion (BHI) Agar; 5425 Composition: Calf brain infusion 200.0 g/l Beef heart infusion 250.0 g/l Casein (meat) peptone 10.0 g/l Glucose 2.0 g/l NaCl 5.0 g/l Na2HPO4 2.5 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.4 Usage: Maintenance and revitalization Organisms: Dermatophilus congolensis, Nocardia brasieliensis, N. asteroides, Rhodococcus equi, R. fascians, R. rhodochrous, Streptomyces thermoviol aceus subsp. apingens, Coryneform species
187465428yes
187465530yes
37340MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
18746positiveoptimum28mesophilic
8819positivegrowth30mesophilic
37340positivegrowth30mesophilic
44732positivegrowth22-30
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44732
  • oxygen tolerance: aerobe

compound production

  • @ref: 20216
  • compound: Putrescine oxidase

murein

  • @ref: 8819
  • murein short key: A11.07
  • type: A3alpha L-Lys-L-Ala4

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837522599arabinose-fermentation
6837532528turanose-fermentation
6837559640N-acetylglucosamine-fermentation
6837517992sucrose-fermentation
6837517632nitrate+reduction
6837517057cellobiose-fermentation
6837516988D-ribose-fermentation
6837516634raffinose-fermentation
6837516899D-mannitol-fermentation
6837527082trehalose-fermentation
6837517716lactose-fermentation
6837517306maltose-fermentation
6837516024D-mannose-fermentation
6837515824D-fructose-fermentation
6837517634D-glucose-fermentation
683754853esculin+hydrolysis
6837518257ornithine-degradation
6837529016arginine-hydrolysis
6837516199urea-hydrolysis
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose-fermentation
6837917306maltose-fermentation
6837916899D-mannitol-fermentation
6837965327D-xylose-fermentation
6837916988D-ribose-fermentation
6837917634D-glucose-fermentation
683795291gelatin-hydrolysis
6837916199urea-hydrolysis
683794853esculin+hydrolysis
6837917632nitrate-reduction
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6837515688acetoin-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase+
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase+3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18746--+---+-+--------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18746+-+-++++------++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18746++++--+----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8819---+----------+--+--------

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
187461Risk group (German classification)
88191Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8819
  • description: M.roseus 16S rRNA gene
  • accession: X87756
  • length: 1481
  • database: ena
  • NCBI tax ID: 1275

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kocuria rosea ATCC 186GCA_006094695completencbi1275
66792Kocuria rosea DSM 20447GCA_006717035contigncbi1275
66792Kocuria rosea ATCC 186GCA_003124035contigncbi1275
66792Kocuria rosea strain ATCC 1861275.9wgspatric1275
66792Kocuria rosea strain ATCC 1861275.17completepatric1275
66792Kocuria rosea strain DSM 204471275.18wgspatric1275
66792Kocuria rosea DSM 204472818991576draftimg1275
67770Kocuria rosea strain ATCC 186 chromosome, complete genomeCP035103ena1275
67770Kocuria rosea strain ATCC 186, whole genome shotgun sequencing projectQFBJ00000000ncbi1275
67770Kocuria rosea strain DSM 20447, whole genome shotgun sequencing projectVFQD00000000ncbi1275

GC content

@refGC-contentmethod
881969.0
6777069thermal denaturation, midpoint method (Tm)

External links

@ref: 8819

culture collection no.: DSM 20447, ATCC 186, CCM 679, NCTC 7523, CCUG 4312, IAM 1315, IFO 3768, JCM 11614, BCRC 11044, CECT 5052, CECT 52, CIP 71.15, IAM 14818, IMSNU 11063, IMSNU 21322, JCM 7236, KCCM 40158, KCTC 3137, KCTC 3199, KCTC 3488, LMG 14224, NBRC 3768, NCFB 975, NCIMB 11696, NRRL B-2977, PCM 548, VKM Ac-2200, VKM B-1236, VKM B-1823

straininfo link

@refpassport
20218http://www.straininfo.net/strains/37032
20218http://www.straininfo.net/strains/37029
20218http://www.straininfo.net/strains/37037

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656171Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample.Reddy GSN, Prakash JSS, Prabahar V, Matsumoto GI, Stackebrandt E, Shivaji SInt J Syst Evol Microbiol10.1099/ijs.0.02336-02003Antarctic Regions, Cyanobacteria/isolation & purification, Ecosystem, Micrococcaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, Pigmentation, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Water MicrobiologyEnzymology
Phylogeny16585685Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt.Li WJ, Zhang YQ, Schumann P, Chen HH, Hozzein WN, Tian XP, Xu LH, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.63876-02006Actinobacteria/chemistry/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/analysis, *Desert Climate, Egypt, Fatty Acids/analysis, Molecular Sequence Data, Peptidoglycan/analysis, Phylogeny, RNA, Ribosomal, 16S/analysis/genetics, Sodium Chloride, *Soil MicrobiologyGenetics
Phylogeny16902039Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas.Mayilraj S, Kroppenstedt RM, Suresh K, Saini HSInt J Syst Evol Microbiol10.1099/ijs.0.63915-02006Actinobacteria/*classification/isolation & purification, Base Composition, DNA, Bacterial/chemistry/genetics, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, *Soil Microbiology, Species SpecificityGenetics
22809716Radio-resistance in psychrotrophic Kocuria sp. ASB 107 isolated from Ab-e-Siah radioactive spring.Asgarani E, Soudi MR, Borzooee F, Dabbagh RJ Environ Radioact10.1016/j.jenvrad.2012.04.0092012Micrococcaceae/*radiation effects, *Radiation, Ionizing, *Ultraviolet Rays
Phylogeny26048314Kocuria dechangensis sp. nov., an actinobacterium isolated from saline and alkaline soils.Wang K, Zhang L, Liu Y, Pan Y, Meng L, Xu T, Zhang C, Liu H, Hong S, Huang H, Jiang JInt J Syst Evol Microbiol10.1099/ijs.0.0003722015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Micrococcaceae/classification, Molecular Sequence Data, Nucleic Acid Hybridization/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/analysis, Soil/chemistry, Soil MicrobiologyGenetics
Phylogeny26362332Kocuria subflava sp. nov., isolated from marine sediment from the Indian Ocean.Jiang Z, Zhang WH, Yuan CG, Chen JY, Cao LX, Park DJ, Xiao M, Kim CJ, Li WJAntonie Van Leeuwenhoek10.1007/s10482-015-0587-z2015Aerobiosis, Amino Acids/analysis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Indian Ocean, Locomotion, Micrococcaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics
26671610On-chip cell lysis by antibacterial non-leaching reusable quaternary ammonium monolithic column.Aly Saad Aly M, Gauthier M, Yeow JBiomed Microdevices10.1007/s10544-015-0025-z2016Allyl Compounds/*chemistry, Anti-Bacterial Agents/*chemistry, Lab-On-A-Chip Devices, Micrococcus luteus/chemistry/*growth & development, Pseudomonas putida/chemistry/*growth & development, Quaternary Ammonium Compounds/*chemistry
Phylogeny27902231Kocuria oceani sp. nov., isolated from a deep-sea hydrothermal plume.Zhang L, Xi L, Ruan J, Huang YInt J Syst Evol Microbiol10.1099/ijsem.0.0015992017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrothermal Vents/*microbiology, Micrococcaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8819Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20447)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20447
18746Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20447.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37340Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10584
44732Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 4312)https://www.ccug.se/strain?id=4312
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68375Automatically annotated from API ID32STA
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym