Strain identifier

BacDive ID: 7631

Type strain: Yes

Species: Citricoccus muralis

Strain Designation: 4-O, 4-0

Strain history: CIP <- 2003, CCM <- H.J. Busse: strain 4-0 <- P. Altenburger

NCBI tax ID(s): 169134 (species)

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General

@ref: 5356

BacDive-ID: 7631

DSM-Number: 14442

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Citricoccus muralis 4-O is an aerobe, mesophilic bacterium that was isolated from medieval wall painting.

NCBI tax id

  • NCBI tax id: 169134
  • Matching level: species

strain history

@refhistory
5356<- H.-J. Busse <- P. Altenburger; 4-0
67770CCM 4981 <-- H.-J. Busse 4-0 <-- P. Altenburger.
121188CIP <- 2003, CCM <- H.J. Busse: strain 4-0 <- P. Altenburger

doi: 10.13145/bacdive7631.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Citricoccus
  • species: Citricoccus muralis
  • full scientific name: Citricoccus muralis Altenburger et al. 2002

@ref: 5356

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Citricoccus

species: Citricoccus muralis

full scientific name: Citricoccus muralis Altenburger et al. 2002

strain designation: 4-O, 4-0

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.677
69480100positive
121188nopositivecoccus-shaped

colony morphology

@refcolony colorincubation periodmedium used
18382Lemon yellow (1012)10-14 daysISP 2
18382Lemon yellow (1012)10-14 daysISP 3
18382Lemon yellow (1012)10-14 daysISP 4
18382Lemon yellow (1012)10-14 daysISP 5
18382Lemon yellow (1012)10-14 daysISP 6
18382Lemon yellow (1012)10-14 daysISP 7
121188

multicellular morphology

@refforms multicellular complexmedium name
18382noISP 2
18382noISP 3
18382noISP 4
18382noISP 5
18382noISP 6
18382noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5356TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18382ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18382ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18382ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18382ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18382ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18382ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39944MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121188CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5356positivegrowth28mesophilic
39944positivegrowth30mesophilic
59306positivegrowth26-30mesophilic
67770positivegrowth28mesophilic
121188positivegrowth10-30
121188nogrowth37mesophilic
121188nogrowth41thermophilic
121188nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59306aerobe
121188obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.987

murein

  • @ref: 5356
  • murein short key: A11.40
  • type: A4alpha L-Lys-Gly-D-Glu

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2), MK-7(H2), MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1838217234glucose+
1838222599arabinose-
1838217992sucrose+
1838218222xylose-
1838217268myo-inositol-
1838229864mannitol-
1838228757fructose-
1838226546rhamnose-
1838216634raffinose-
1838262968cellulose-
121188606565hippurate+hydrolysis
12118817632nitrate-reduction
12118816301nitrite-reduction
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121188
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 121188
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
121188oxidase-
121188beta-galactosidase-3.2.1.23
121188alcohol dehydrogenase-1.1.1.1
121188gelatinase+/-
121188amylase+
121188DNase-
121188caseinase-3.4.21.50
121188catalase+1.11.1.6
121188tween esterase+
121188gamma-glutamyltransferase-2.3.2.2
121188lysine decarboxylase-4.1.1.18
121188ornithine decarboxylase-4.1.1.17
121188phenylalanine ammonia-lyase-4.3.1.24
121188urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18382-+++--+--+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18382+++-+--+-+----+----
121188-+--++-+--+---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121188----------------+--------------------------------+----------++++--+--+-----+---++-------++++--+++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5356medieval wall paintingSt. Georgen ob JudenburgAustriaAUTEurope
59306Medieval wall paintingStyria,St.Georgen ob JudenburgAustriaAUTEurope
67770Medieval wall painting in the church of St. GeorgenStyriaAustriaAUTEurope
121188Medieval wall painting churchAustriaAUTEurope1993

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Painting
#Engineered#Built environment

taxonmaps

  • @ref: 69479
  • File name: preview.99_48118.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_453;97_3040;98_6467;99_48118&stattab=map
  • Last taxonomy: Micrococcus
  • 16S sequence: AJ344143
  • Sequence Identity:
  • Total samples: 27
  • soil counts: 11
  • aquatic counts: 2
  • animal counts: 13
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53561Risk group (German classification)
183821
1211881Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5356
  • description: Citrococcus muralis 16S rRNA gene, strain 4-0
  • accession: AJ344143
  • length: 1455
  • database: ena
  • NCBI tax ID: 169134

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Citricoccus muralis strain DSM 14442169134.3wgspatric169134
66792Citricoccus muralis DSM 144422773857773draftimg169134
67770Citricoccus muralis DSM 14442GCA_003386075contigncbi169134

GC content

  • @ref: 5356
  • GC-content: 68
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.487no
gram-positiveyes94.006no
anaerobicno99.38yes
aerobicyes94.446yes
halophileno50no
spore-formingno90.872no
glucose-utilyes87.163yes
flagellatedno98.19no
thermophileno98.788yes
glucose-fermentno88.598yes

External links

@ref: 5356

culture collection no.: DSM 14442, CCM 4981, NBRC 106156, CCUG 51768, JCM 12134, CIP 107799, DSM 11442

straininfo link

  • @ref: 77075
  • straininfo: 100598

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12508874Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.Altenburger P, Kampfer P, Schumann P, Steiner R, Lubitz W, Busse HJInt J Syst Evol Microbiol10.1099/00207713-52-6-20952002Austria, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Environmental Microbiology, Lipids/analysis, Micrococcaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Paintings, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicGenetics
Phylogeny15653858Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt.Li WJ, Chen HH, Zhang YQ, Kim CJ, Park DJ, Lee JC, Xu LH, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.63237-02005Bacterial Typing Techniques, Benzoquinones/analysis, DNA, Ribosomal/analysis, *Desert Climate, Egypt, Fatty Acids/analysis, Hydrogen-Ion Concentration, Lipids/analysis, Micrococcaceae/chemistry/*classification/genetics/*isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny19651738Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005.Schafer J, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.012567-02009Base Sequence, DNA, Bacterial/genetics, *Environmental Microbiology, Genes, Bacterial/genetics, Micrococcaceae/*classification/genetics/physiology, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species SpecificityGenetics
Phylogeny19654363Citricoccus zhacaiensis sp. nov., isolated from a bioreactor for saline wastewater treatment.Meng FX, Yang XC, Yu PS, Pan JM, Wang CS, Xu XW, Wu MInt J Syst Evol Microbiol10.1099/ijs.0.011635-02009Bioreactors/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Micrococcaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Sodium Chloride/metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5356Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14442)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14442
18382Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM14442.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39944Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5274
59306Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51768)https://www.ccug.se/strain?id=51768
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77075Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100598.1StrainInfo: A central database for resolving microbial strain identifiers
121188Curators of the CIPCollection of Institut Pasteur (CIP 107799)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107799