Strain identifier

BacDive ID: 7606

Type strain: Yes

Species: Arthrobacter monumenti

Strain Designation: R-5344

Strain history: CIP <- 2005, DSMZ

NCBI tax ID(s): 163875 (species)

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General

@ref: 6434

BacDive-ID: 7606

DSM-Number: 16405

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Arthrobacter monumenti R-5344 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Biofilm covering the Servilia tomb, Roman necropolis of Carmona.

NCBI tax id

  • NCBI tax id: 163875
  • Matching level: species

strain history

@refhistory
6434<- J. Heyrman, R-5344
343572005, DSMZ
67770IAM 15321 <-- LMG 19502 <-- J. Heyrman R-5344.
121134CIP <- 2005, DSMZ

doi: 10.13145/bacdive7606.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter monumenti
  • full scientific name: Arthrobacter monumenti Heyrman et al. 2005

@ref: 6434

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter monumenti

full scientific name: Arthrobacter monumenti Heyrman et al. 2005

strain designation: R-5344

type strain: yes

Morphology

cell morphology

@refgram staincell widthcell shapemotility
31342positive0.9 µmcoccus-shapedno
121134positivecoccus-shapedno

colony morphology

  • @ref: 121134

pigmentation

  • @ref: 31342
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6434BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
34357MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121134CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328
121134CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6434positivegrowth28mesophilic
31342positivegrowth04-37
31342positiveoptimum26mesophilic
34357positivegrowth30mesophilic
67770positivegrowth28mesophilic
121134positivegrowth10-30
121134nogrowth37mesophilic
121134nogrowth41thermophilic
121134nogrowth45thermophilic

culture pH

  • @ref: 31342
  • ability: positive
  • type: optimum
  • pH: 7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31342aerobe
121134obligate aerobe

spore formation

  • @ref: 31342
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31342NaClpositivegrowth05-15 %
121134NaClpositivegrowth0-2 %
121134NaClnogrowth4 %
121134NaClnogrowth6 %
121134NaClnogrowth8 %
121134NaClnogrowth10 %

murein

  • @ref: 6434
  • murein short key: A11.07
  • type: A3alpha L-Lys-L-Ala4

observation

@refobservation
31342aggregates in clumps
67770quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
313425291gelatin+carbon source
313424853esculin+hydrolysis
3134217632nitrate+reduction
12113416947citrate-carbon source
1211344853esculin+hydrolysis
121134606565hippurate+hydrolysis
12113417632nitrate+reduction
12113416301nitrite-reduction

metabolite production

  • @ref: 121134
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12113415688acetoin-
12113417234glucose-

enzymes

@refvalueactivityec
31342alkaline phosphatase+3.1.3.1
31342catalase+1.11.1.6
31342gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121134oxidase-
121134beta-galactosidase+3.2.1.23
121134alcohol dehydrogenase-1.1.1.1
121134gelatinase+
121134amylase+
121134DNase-
121134caseinase+3.4.21.50
121134catalase+1.11.1.6
121134tween esterase-
121134gamma-glutamyltransferase+2.3.2.2
121134lecithinase-
121134lipase-
121134lysine decarboxylase-4.1.1.18
121134ornithine decarboxylase-4.1.1.17
121134phenylalanine ammonia-lyase+4.3.1.24
121134tryptophan deaminase-
121134urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121134--++-+-------+-+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6434Biofilm covering the Servilia tomb, Roman necropolis of CarmonaCarmonaSpainESPEurope
67770Biofilm overgrowing a mural painting in the Servilia tombRoman necropolis of CarmonaSpainESPEurope
121134Mural paintings sitesServilia tomb, CarmonaSpainESPEurope

isolation source categories

Cat1Cat2
#Environmental#Biofilm
#Environmental#Terrestrial

taxonmaps

  • @ref: 69479
  • File name: preview.99_78475.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1023;97_41964;98_54782;99_78475&stattab=map
  • Last taxonomy: Arthrobacter monumenti subclade
  • 16S sequence: AJ315070
  • Sequence Identity:
  • Total samples: 1203
  • soil counts: 701
  • aquatic counts: 90
  • animal counts: 230
  • plant counts: 182

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64341Risk group (German classification)
1211341Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6434
  • description: Arthrobacter monumenti 16S rRNA gene, type strain LMG 19502T
  • accession: AJ315070
  • length: 1484
  • database: ena
  • NCBI tax ID: 163875

GC content

@refGC-contentmethod
643462.2
6777062.2high performance liquid chromatography (HPLC)

External links

@ref: 6434

culture collection no.: DSM 16405, LMG 19502, JCM 21770, IAM 15321, CIP 108897

straininfo link

  • @ref: 77050
  • straininfo: 13192

literature

  • topic: Phylogeny
  • Pubmed-ID: 16014466
  • title: Six novel Arthrobacter species isolated from deteriorated mural paintings.
  • authors: Heyrman J, Verbeeren J, Schumann P, Swings J, De Vos P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63358-0
  • year: 2005
  • mesh: Arthrobacter/chemistry/*classification/genetics/*isolation & purification, Austria, Bacterial Typing Techniques, Base Composition, Biofilms/*growth & development, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, *Paintings, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6434Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16405)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16405
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31342Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2765628776041
34357Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6578
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77050Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13192.1StrainInfo: A central database for resolving microbial strain identifiers
121134Curators of the CIPCollection of Institut Pasteur (CIP 108897)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108897