Strain identifier
BacDive ID: 76
Type strain:
Species: Roseomonas aerilata
Strain Designation: 5420S-30
Strain history: CIP <- 2010, KACC: strain 5420S-30 <- H.-Y. Weon, Natl. Inst. Agricult. Sci. and Technol., Suwon, South Korea
NCBI tax ID(s): 1123060 (strain), 452982 (species)
General
@ref: 8074
BacDive-ID: 76
DSM-Number: 19363
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming
description: Roseomonas aerilata 5420S-30 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from air.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1123060 | strain |
452982 | species |
strain history
@ref | history |
---|---|
40348 | 2010, KACC |
8074 | <- S.-W. Kwon, KACC; 5420S-30 <- H.-Y. Weon, Natl. Inst. Agricult. Sci. and Technol., Suwon, Korea |
67772 | Kwon S.-W., Korean Agricultural Culture Collection; 5420S-30 < Weon H.-Y., National Institute of Agricultural Science and Technology |
116059 | CIP <- 2010, KACC: strain 5420S-30 <- H.-Y. Weon, Natl. Inst. Agricult. Sci. and Technol., Suwon, South Korea |
doi: 10.13145/bacdive76.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Roseomonas
- species: Roseomonas aerilata
- full scientific name: Roseomonas aerilata Yoo et al. 2008
synonyms
- @ref: 20215
- synonym: Pararoseomonas aerilata
@ref: 8074
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Roseomonas
species: Roseomonas aerilata
full scientific name: Roseomonas aerilata Yoo et al. 2008
strain designation: 5420S-30
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell shape | motility | confidence |
---|---|---|---|---|---|
22961 | negative | 1.2-1.5 µm | coccus-shaped | no | |
69480 | negative | 99.996 | |||
116059 | negative | coccus-shaped | no |
colony morphology
- @ref: 22961
- colony color: pink
- colony shape: circular
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8074 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
22961 | Nutrient agar (NA) | yes | ||
22961 | Reasoner's 2A agar (R2A) | yes | ||
40348 | MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati | yes | Distilled water make up to (1000.000 ml);R2A agar (18.200 g) | |
116059 | CIP Medium 566 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8074 | positive | growth | 30 | mesophilic |
22961 | positive | growth | 5.0-35.0 | |
22961 | positive | optimum | 30.0 | mesophilic |
40348 | positive | growth | 30 | mesophilic |
67772 | positive | minimum | 5 | psychrophilic |
67772 | positive | optimum | 30 | mesophilic |
67772 | positive | maximum | 35 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
22961 | positive | growth | 5.0-9.0 | alkaliphile |
22961 | positive | optimum | 6.0-7.0 | |
67772 | positive | optimum | 6.0-7.0 | |
67772 | positive | maximum | 9.0 | |
67772 | positive | minimum | 5.0 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
22961 | obligate aerobe |
116059 | obligate aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.972 |
halophily
- @ref: 22961
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: >1.0 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22961 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
22961 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
22961 | 27613 | amygdalin | - | builds acid from |
22961 | 18305 | arbutin | - | builds acid from |
22961 | 15963 | ribitol | - | builds acid from |
22961 | 17108 | D-arabinose | - | builds acid from |
22961 | 18333 | D-arabitol | - | builds acid from |
22961 | 17057 | cellobiose | - | builds acid from |
22961 | 15824 | D-fructose | - | builds acid from |
22961 | 28847 | D-fucose | - | builds acid from |
22961 | 12936 | D-galactose | - | builds acid from |
22961 | 17634 | D-glucose | - | builds acid from |
22961 | 17716 | lactose | - | builds acid from |
22961 | 62318 | D-lyxose | - | builds acid from |
22961 | 17306 | maltose | - | builds acid from |
22961 | 16899 | D-mannitol | - | builds acid from |
22961 | 16024 | D-mannose | - | builds acid from |
22961 | 6731 | melezitose | - | builds acid from |
22961 | 28053 | melibiose | - | builds acid from |
22961 | 16634 | raffinose | - | builds acid from |
22961 | 16988 | D-ribose | - | builds acid from |
22961 | 17992 | sucrose | - | builds acid from |
22961 | 17924 | D-sorbitol | - | builds acid from |
22961 | 16443 | D-tagatose | - | builds acid from |
22961 | 16551 | D-trehalose | - | builds acid from |
22961 | 32528 | turanose | - | builds acid from |
22961 | 65327 | D-xylose | - | builds acid from |
22961 | 16813 | galactitol | - | builds acid from |
22961 | 17113 | erythritol | - | builds acid from |
22961 | 4853 | esculin | - | builds acid from |
22961 | 28066 | gentiobiose | - | builds acid from |
22961 | 24265 | gluconate | - | builds acid from |
22961 | 17754 | glycerol | - | builds acid from |
22961 | 28087 | glycogen | - | builds acid from |
22961 | 17268 | myo-inositol | - | builds acid from |
22961 | 15443 | inulin | - | builds acid from |
22961 | 30849 | L-arabinose | - | builds acid from |
22961 | 18403 | L-arabitol | - | builds acid from |
22961 | 18287 | L-fucose | - | builds acid from |
22961 | 62345 | L-rhamnose | - | builds acid from |
22961 | 17266 | L-sorbose | - | builds acid from |
22961 | 65328 | L-xylose | - | builds acid from |
22961 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
22961 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
22961 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
22961 | 506227 | N-acetylglucosamine | - | builds acid from |
22961 | 17814 | salicin | - | builds acid from |
22961 | 28017 | starch | - | builds acid from |
22961 | 17151 | xylitol | - | builds acid from |
22961 | 29016 | arginine | - | carbon source |
22961 | 85146 | carboxymethylcellulose | - | degradation |
22961 | casein | - | degradation | |
22961 | 17029 | chitin | - | degradation |
22961 | 16991 | dna | - | degradation |
22961 | 17368 | hypoxanthine | - | degradation |
22961 | 17309 | pectin | - | degradation |
22961 | 28017 | starch | - | degradation |
22961 | 18186 | tyrosine | - | degradation |
22961 | 15318 | xanthine | - | degradation |
22961 | 17234 | glucose | - | fermentation |
22961 | 17128 | adipate | - | growth |
22961 | 27689 | decanoate | - | growth |
22961 | 17634 | D-glucose | - | growth |
22961 | 17306 | maltose | - | growth |
22961 | 16899 | D-mannitol | - | growth |
22961 | 16024 | D-mannose | - | growth |
22961 | 24297 | glucuronate | - | growth |
22961 | 30849 | L-arabinose | - | growth |
22961 | 25115 | malate | - | growth |
22961 | 506227 | N-acetylglucosamine | - | growth |
22961 | 18401 | phenylacetate | - | growth |
22961 | 53258 | sodium citrate | - | growth |
22961 | 4853 | esculin | - | hydrolysis |
22961 | 5291 | gelatin | - | hydrolysis |
22961 | 16199 | urea | - | hydrolysis |
22961 | 17632 | nitrate | - | reduction |
22961 | 53426 | tween 80 | + | degradation |
22961 | 16193 | 3-hydroxybenzoate | + | growth |
22961 | 37054 | 3-hydroxybutyrate | + | growth |
22961 | 9300 | suberic acid | + | growth |
116059 | 17632 | nitrate | - | reduction |
116059 | 16301 | nitrite | - | reduction |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive | sensitivity conc. |
---|---|---|---|---|---|---|---|
22961 | 8309 | polymyxin b | yes | yes | 300 Unit | ||
22961 | 28971 | ampicillin | yes | yes | 10 µg | ||
22961 | 71321 | fusidate | yes | yes | 10 µg | ||
22961 | 6472 | lincomycin | yes | yes | 15 µg | ||
22961 | 28001 | vancomycin | yes | yes | 30 µg | ||
22961 | 17334 | penicillin | yes | yes | 10 Unit | ||
22961 | 17833 | gentamicin | yes | yes | 10 µg | ||
22961 | 17076 | streptomycin | yes | yes | 10 µg | ||
22961 | 48923 | erythromycin | yes | yes | 15 µg | ||
22961 | 2637 | amikacin | yes | yes | 30 µg | ||
22961 | 3542 | cephalothin | yes | yes | 30 µg | ||
22961 | 204928 | cefotaxime | yes | yes | 30 µg | ||
22961 | 17698 | chloramphenicol | yes | yes | 30 µg | ||
22961 | 6104 | kanamycin | yes | yes | 30 µg | ||
22961 | 100147 | nalidixic acid | yes | yes | 30 µg | ||
22961 | 7507 | neomycin | yes | yes | 30 µg | ||
22961 | 27902 | tetracycline | yes | yes | 30 µg | ||
22961 | 100241 | ciprofloxacin | yes | yes | 5 µg | ||
22961 | 28077 | rifampicin | yes | yes | 5 µg |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
22961 | 35581 | indole | no |
116059 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
22961 | acid phosphatase | + | 3.1.3.2 |
22961 | alkaline phosphatase | + | 3.1.3.1 |
22961 | alpha-chymotrypsin | - | 3.4.21.1 |
22961 | alpha-fucosidase | - | 3.2.1.51 |
22961 | alpha-galactosidase | - | 3.2.1.22 |
22961 | alpha-glucosidase | - | 3.2.1.20 |
22961 | alpha-mannosidase | - | 3.2.1.24 |
22961 | beta-galactosidase | - | 3.2.1.23 |
22961 | beta-glucuronidase | - | 3.2.1.31 |
22961 | catalase | + | 1.11.1.6 |
22961 | cystine arylamidase | - | 3.4.11.3 |
22961 | cytochrome oxidase | + | 1.9.3.1 |
22961 | esterase (C 4) | + | |
22961 | esterase lipase (C 8) | + | |
22961 | leucine arylamidase | - | 3.4.11.1 |
22961 | lipase (C 14) | - | |
22961 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
22961 | naphthol-AS-BI-phosphohydrolase | + | |
22961 | trypsin | - | 3.4.21.4 |
22961 | urease | - | 3.5.1.5 |
22961 | valine arylamidase | - | |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
116059 | oxidase | + | |
116059 | catalase | + | 1.11.1.6 |
116059 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116059 | - | - | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature | sampling date |
---|---|---|---|---|---|---|---|---|---|---|---|
8074 | air | Suwon (37° 16' 46'' N 126° 59' 10'' E) | Republic of Korea | KOR | Asia | 37.2794 | 126.986 | ||||
22961 | R2A agar with 200 mg cycloheximide ml 21 | 5 days | 30 | ||||||||
67772 | Air sample | Gyeonggi-do, Suwon | Republic of Korea | KOR | Asia | 37.2794 | 126.986 | 2005 | |||
116059 | Environment, Air sample | Suwon | Republic of Korea | KOR | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Air
taxonmaps
- @ref: 69479
- File name: preview.99_83866.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_266;96_1896;97_9131;98_11193;99_83866&stattab=map
- Last taxonomy: Roseomonas aerilata
- 16S sequence: EF661571
- Sequence Identity:
- Total samples: 270
- soil counts: 53
- aquatic counts: 58
- animal counts: 39
- plant counts: 120
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
8074 | 1 | Risk group (German classification) |
67772 | 1 | |
116059 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 8074
- description: Roseomonas aerilata strain 5420S-30 16S ribosomal RNA gene, partial sequence
- accession: EF661571
- length: 1354
- database: ena
- NCBI tax ID: 1123060
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Roseomonas aerilata DSM 19363 | 1123060.3 | wgs | patric | 1123060 |
66792 | Roseomonas aerilata DSM 19363 | 2565956516 | draft | img | 1123060 |
67772 | Roseomonas aerilata DSM 19363 | GCA_000711725 | contig | ncbi | 1123060 |
GC content
@ref | GC-content | method |
---|---|---|
8074 | 72.1 | |
22961 | 72.1 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 67.552 | yes |
flagellated | no | 91.089 | yes |
gram-positive | no | 98.072 | yes |
anaerobic | no | 98.783 | no |
aerobic | yes | 94.048 | no |
halophile | no | 90.656 | no |
spore-forming | no | 94.984 | no |
glucose-util | yes | 75.825 | yes |
thermophile | no | 97.091 | yes |
glucose-ferment | no | 91.409 | yes |
External links
@ref: 8074
culture collection no.: DSM 19363, KACC 12521, UCCCB24, CIP 110193
straininfo link
- @ref: 69760
- straininfo: 354323
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 18523199 | Roseomonas aerilata sp. nov., isolated from an air sample. | Yoo SH, Weon HY, Noh HJ, Hong SB, Lee CM, Kim BY, Kwon SW, Go SJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.65385-0 | 2008 | *Air Microbiology, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Korea, Methylobacteriaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity | Genetics |
Phylogeny | 27983479 | Roseomonas nepalensis sp. nov., isolated from oil-contaminated soil. | Chaudhary DK, Kim J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001727 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylobacteriaceae/*classification/genetics/isolation & purification, Nepal, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry | Transcriptome |
Phylogeny | 28983692 | Roseomonas aeriglobus sp. nov., isolated from an air-conditioning system. | Lee Y, Jeon CO | Antonie Van Leeuwenhoek | 10.1007/s10482-017-0956-x | 2017 | *Air Microbiology, *Bacterial Typing Techniques, Base Composition, *Environmental Microbiology, Methylobacteriaceae/*classification/isolation & purification, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Phenotype |
Phylogeny | 29458474 | Roseomonas fluminis sp. nov. isolated from sediment of a shallow stream. | Ko Y, Yim J, Hwang WM, Kang K, Ahn TY | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002578 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Methylobacteriaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phosphatidylglycerols/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rivers/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8074 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19363) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19363 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
22961 | Seung-Hee Yoo,Hang-Yeon Weon,Hyung-Jun Noh,Seung-Beom Hong,Chang-Muk Lee,Byung-Yong Kim,Soon-Wo Kwon,Seung-Joo Go | 10.1099/ijs.0.65385-0 | Roseomonas aerilata sp. nov., isolated from an air sample | IJSEM 58: 1482-1485 2008 | 18523199 | |
40348 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/8026 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67772 | Curators of the UCCCB | https://ucccb.uc.pt/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
69760 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID354323.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116059 | Curators of the CIP | Collection of Institut Pasteur (CIP 110193) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110193 |