Strain identifier

BacDive ID: 7598

Type strain: Yes

Species: Arthrobacter psychrolactophilus

Strain Designation: B7

Strain history: CIP <- 2002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7

NCBI tax ID(s): 92442 (species)

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General

@ref: 5930

BacDive-ID: 7598

DSM-Number: 15612

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Arthrobacter psychrolactophilus B7 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from soil from whey-enriched farm field.

NCBI tax id

  • NCBI tax id: 92442
  • Matching level: species

strain history

@refhistory
5930<- ATCC <- J. E. Brenchley; B7 <- K. R. Gutshall
373892002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7
67770CIP 107481 <-- J. E. Brenchley B7.
122017CIP <- 2002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7

doi: 10.13145/bacdive7598.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter psychrolactophilus
  • full scientific name: Arthrobacter psychrolactophilus Loveland-Curtze et al. 2000

@ref: 5930

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter psychrolactophilus

full scientific name: Arthrobacter psychrolactophilus Loveland-Curtze et al. 2000

strain designation: B7

type strain: yes

Morphology

cell morphology

  • @ref: 122017
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 122017

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5930TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37389MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122017CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5930positivegrowth28mesophilic
37389positivegrowth25mesophilic
67770positivegrowth28mesophilic
122017positivegrowth10-25psychrophilic
122017nogrowth30mesophilic
122017nogrowth37mesophilic
122017nogrowth41thermophilic
122017nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122017
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122017NaClpositivegrowth0-2 %
122017NaClnogrowth4 %
122017NaClnogrowth6 %
122017NaClnogrowth8 %
122017NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2), MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12201716947citrate-carbon source
1220174853esculin+hydrolysis
122017606565hippurate+hydrolysis
12201717632nitrate-reduction
12201716301nitrite-reduction
12201717632nitrate-respiration

antibiotic resistance

  • @ref: 122017
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122017
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12201715688acetoin+
12201717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
122017oxidase-
122017beta-galactosidase+3.2.1.23
122017alcohol dehydrogenase-1.1.1.1
122017gelatinase+/-
122017amylase+
122017DNase-
122017caseinase-3.4.21.50
122017catalase+1.11.1.6
122017tween esterase-
122017gamma-glutamyltransferase-2.3.2.2
122017lecithinase-
122017lipase-
122017lysine decarboxylase-4.1.1.18
122017ornithine decarboxylase-4.1.1.17
122017tryptophan deaminase-
122017urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122017--++-+-----+-+------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122017+++-+-++++++++-++++-+++-+++----+-++++-+----------++---+-++-++----++-----++-+--+++-----+--++-----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5930soil from whey-enriched farm fieldPennsylvaniaUSAUSANorth America
67770Soil from whey-enriched farm fieldUSAUSANorth America
122017Environment, SoilPennsylvaniaUnited States of AmericaUSANorth America1992

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_12557.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_773;97_901;98_1057;99_12557&stattab=map
  • Last taxonomy: Arthrobacter
  • 16S sequence: LC073757
  • Sequence Identity:
  • Total samples: 9379
  • soil counts: 3611
  • aquatic counts: 1646
  • animal counts: 3144
  • plant counts: 978

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59301Risk group (German classification)
1220171Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5930Arthrobacter psychrolactophilus 16S ribosomal RNA gene, partial sequenceAF1341791414ena92442
67770Arthrobacter psychrolactophilus gene for 16S ribosomal RNA, partial sequence, strain: JCM 12399LC0737571475ena92442

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter psychrolactophilus B72866272416draftimg92442
67770Arthrobacter psychrolactophilus B7GCA_003219795contigncbi92442

GC content

@refGC-contentmethod
593060.6
6777060.6thermal denaturation, midpoint method (Tm)

External links

@ref: 5930

culture collection no.: DSM 15612, ATCC 700733, JCM 12399, CIP 107481

straininfo link

  • @ref: 77043
  • straininfo: 44110

literature

  • topic: Metabolism
  • Pubmed-ID: 15931519
  • title: Production of amylase by Arthrobacter psychrolactophilus.
  • authors: Smith MR, Zahnley JC
  • journal: J Ind Microbiol Biotechnol
  • DOI: 10.1007/s10295-005-0240-3
  • year: 2005
  • mesh: Amylases/biosynthesis/chemistry/*metabolism, Arthrobacter/*enzymology, Bacterial Proteins/biosynthesis/chemistry/*metabolism, Enzyme Stability, Starch/metabolism, Temperature, Time Factors
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5930Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15612)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15612
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37389Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4921
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77043Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44110.1StrainInfo: A central database for resolving microbial strain identifiers
122017Curators of the CIPCollection of Institut Pasteur (CIP 107481)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107481