Strain identifier
BacDive ID: 7598
Type strain:
Species: Arthrobacter psychrolactophilus
Strain Designation: B7
Strain history: CIP <- 2002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7
NCBI tax ID(s): 92442 (species)
General
@ref: 5930
BacDive-ID: 7598
DSM-Number: 15612
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped
description: Arthrobacter psychrolactophilus B7 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from soil from whey-enriched farm field.
NCBI tax id
- NCBI tax id: 92442
- Matching level: species
strain history
@ref | history |
---|---|
5930 | <- ATCC <- J. E. Brenchley; B7 <- K. R. Gutshall |
37389 | 2002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7 |
67770 | CIP 107481 <-- J. E. Brenchley B7. |
122017 | CIP <- 2002, J. Brenchley, Pennstate Univ., Pennsylvania, USA, strain: B7 |
doi: 10.13145/bacdive7598.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Micrococcaceae
- genus: Arthrobacter
- species: Arthrobacter psychrolactophilus
- full scientific name: Arthrobacter psychrolactophilus Loveland-Curtze et al. 2000
@ref: 5930
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Micrococcaceae
genus: Arthrobacter
species: Arthrobacter psychrolactophilus
full scientific name: Arthrobacter psychrolactophilus Loveland-Curtze et al. 2000
strain designation: B7
type strain: yes
Morphology
cell morphology
- @ref: 122017
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 122017
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5930 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
37389 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
122017 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5930 | positive | growth | 28 | mesophilic |
37389 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
122017 | positive | growth | 10-25 | psychrophilic |
122017 | no | growth | 30 | mesophilic |
122017 | no | growth | 37 | mesophilic |
122017 | no | growth | 41 | thermophilic |
122017 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 122017
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
122017 | NaCl | positive | growth | 0-2 % |
122017 | NaCl | no | growth | 4 % |
122017 | NaCl | no | growth | 6 % |
122017 | NaCl | no | growth | 8 % |
122017 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: MK-9(H2), MK-8(H2)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
122017 | 16947 | citrate | - | carbon source |
122017 | 4853 | esculin | + | hydrolysis |
122017 | 606565 | hippurate | + | hydrolysis |
122017 | 17632 | nitrate | - | reduction |
122017 | 16301 | nitrite | - | reduction |
122017 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 122017
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
- @ref: 122017
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
122017 | 15688 | acetoin | + | |
122017 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
122017 | oxidase | - | |
122017 | beta-galactosidase | + | 3.2.1.23 |
122017 | alcohol dehydrogenase | - | 1.1.1.1 |
122017 | gelatinase | +/- | |
122017 | amylase | + | |
122017 | DNase | - | |
122017 | caseinase | - | 3.4.21.50 |
122017 | catalase | + | 1.11.1.6 |
122017 | tween esterase | - | |
122017 | gamma-glutamyltransferase | - | 2.3.2.2 |
122017 | lecithinase | - | |
122017 | lipase | - | |
122017 | lysine decarboxylase | - | 4.1.1.18 |
122017 | ornithine decarboxylase | - | 4.1.1.17 |
122017 | tryptophan deaminase | - | |
122017 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122017 | - | - | + | + | - | + | - | - | - | - | - | + | - | + | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122017 | + | + | + | - | + | - | + | + | + | + | + | + | + | + | - | + | + | + | + | - | + | + | + | - | + | + | + | - | - | - | - | + | - | + | + | + | + | - | + | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | + | - | + | + | - | + | + | - | - | - | - | + | + | - | - | - | - | - | + | + | - | + | - | - | + | + | + | - | - | - | - | - | + | - | - | + | + | - | - | - | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
5930 | soil from whey-enriched farm field | Pennsylvania | USA | USA | North America | |
67770 | Soil from whey-enriched farm field | USA | USA | North America | ||
122017 | Environment, Soil | Pennsylvania | United States of America | USA | North America | 1992 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Field |
#Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_12557.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_773;97_901;98_1057;99_12557&stattab=map
- Last taxonomy: Arthrobacter
- 16S sequence: LC073757
- Sequence Identity:
- Total samples: 9379
- soil counts: 3611
- aquatic counts: 1646
- animal counts: 3144
- plant counts: 978
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5930 | 1 | Risk group (German classification) |
122017 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
5930 | Arthrobacter psychrolactophilus 16S ribosomal RNA gene, partial sequence | AF134179 | 1414 | ena | 92442 |
67770 | Arthrobacter psychrolactophilus gene for 16S ribosomal RNA, partial sequence, strain: JCM 12399 | LC073757 | 1475 | ena | 92442 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Arthrobacter psychrolactophilus B7 | 2866272416 | draft | img | 92442 |
67770 | Arthrobacter psychrolactophilus B7 | GCA_003219795 | contig | ncbi | 92442 |
GC content
@ref | GC-content | method |
---|---|---|
5930 | 60.6 | |
67770 | 60.6 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 5930
culture collection no.: DSM 15612, ATCC 700733, JCM 12399, CIP 107481
straininfo link
- @ref: 77043
- straininfo: 44110
literature
- topic: Metabolism
- Pubmed-ID: 15931519
- title: Production of amylase by Arthrobacter psychrolactophilus.
- authors: Smith MR, Zahnley JC
- journal: J Ind Microbiol Biotechnol
- DOI: 10.1007/s10295-005-0240-3
- year: 2005
- mesh: Amylases/biosynthesis/chemistry/*metabolism, Arthrobacter/*enzymology, Bacterial Proteins/biosynthesis/chemistry/*metabolism, Enzyme Stability, Starch/metabolism, Temperature, Time Factors
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5930 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15612) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15612 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
37389 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4921 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
77043 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44110.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
122017 | Curators of the CIP | Collection of Institut Pasteur (CIP 107481) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107481 |