Strain identifier

BacDive ID: 7596

Type strain: Yes

Species: Arthrobacter flavus

Strain history: CIP <- 2002, S. Shivaji, CCMB, Hyderabad, India: strain CMS 19y

NCBI tax ID(s): 95172 (species)

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General

@ref: 5844

BacDive-ID: 7596

DSM-Number: 15322

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-positive, rod-shaped

description: Arthrobacter flavus DSM 15322 is an obligate aerobe, psychrophilic, Gram-positive bacterium that was isolated from cyanobacterial mat sample.

NCBI tax id

  • NCBI tax id: 95172
  • Matching level: species

strain history

@refhistory
5844<- S. Shivaji; CMS 19Y
373942002, S. Shivaji, CCMB, Hyderabad, India: strain CMS 19y
67770MTCC 3476 <-- S. Shivaji CMS 19Y.
119385CIP <- 2002, S. Shivaji, CCMB, Hyderabad, India: strain CMS 19y

doi: 10.13145/bacdive7596.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter flavus
  • full scientific name: Arthrobacter flavus Reddy et al. 2000

@ref: 5844

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter flavus

full scientific name: Arthrobacter flavus Reddy et al. 2000

type strain: yes

Morphology

cell morphology

  • @ref: 119385
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119385

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5844NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605)yeshttps://mediadive.dsmz.de/medium/605Name: NUTRIENT AGAR (OXOID CM3) (DSMZ Medium 605) Composition: Agar 15.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Yeast extract 2.0 g/l Lab-Lemco beef extract 1.0 g/l Distilled water
37394MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119385CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5844positivegrowth22psychrophilic
37394positivegrowth25mesophilic
67770positivegrowth20psychrophilic
119385positivegrowth15-30
119385nogrowth10psychrophilic
119385nogrowth37mesophilic
119385nogrowth41thermophilic
119385nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119385
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119385NaClpositivegrowth0-4 %
119385NaClnogrowth6 %
119385NaClnogrowth8 %
119385NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11938516947citrate-carbon source
1193854853esculin-hydrolysis
119385606565hippurate+hydrolysis
11938517632nitrate+reduction
11938516301nitrite-reduction

antibiotic resistance

  • @ref: 119385
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 119385
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11938515688acetoin-
11938517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119385oxidase-
119385beta-galactosidase+3.2.1.23
119385alcohol dehydrogenase-1.1.1.1
119385gelatinase+/-
119385amylase+
119385DNase+
119385caseinase-3.4.21.50
119385catalase+1.11.1.6
119385tween esterase-
119385gamma-glutamyltransferase-2.3.2.2
119385lecithinase-
119385lipase-
119385lysine decarboxylase-4.1.1.18
119385ornithine decarboxylase-4.1.1.17
119385phenylalanine ammonia-lyase-4.3.1.24
119385tryptophan deaminase-
119385urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119385--++-+--+--++--+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
5844cyanobacterial mat sampleMcMurdoAustralia and Oceania
67770Cyanobacterial mat from a pond in Antarctica
119385Sample, cyanobacterial materialAntarcticaAntarcticaATA

isolation source categories

Cat1Cat2Cat3
#Environmental#Microbial community#Microbial mat
#Host#Microbial#Bacteria

taxonmaps

  • @ref: 69479
  • File name: preview.99_1388.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_803;97_937;98_1097;99_1388&stattab=map
  • Last taxonomy: Arthrobacter
  • 16S sequence: AB299278
  • Sequence Identity:
  • Total samples: 312
  • soil counts: 55
  • aquatic counts: 42
  • animal counts: 212
  • plant counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58441Risk group (German classification)
1193851Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5844Arthrobacter haridrum partial 16S rRNA gene, isolate CMS-19YAJ2425321446ena95172
67770Arthrobacter flavus gene for 16S rRNA, partial sequenceAB2992781377ena95172
67770Arthrobacter flavus gene for 16S ribosomal RNA, partial sequenceAB5371681458ena95172

GC content

@refGC-contentmethod
584464
6777062-66thermal denaturation, midpoint method (Tm)

External links

@ref: 5844

culture collection no.: DSM 15322, MTCC 3476, CMS 19Y, JCM 11496, CIP 107766, KCTC 9998

straininfo link

  • @ref: 77041
  • straininfo: 100510

literature

  • topic: Phylogeny
  • Pubmed-ID: 10939663
  • title: Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica.
  • authors: Reddy GS, Aggarwal RK, Matsumoto GI, Shivaji S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-4-1553
  • year: 2000
  • mesh: Antarctic Regions, Arthrobacter/chemistry/*classification/physiology, Base Composition, Cell Wall/chemistry, Culture Media, DNA, Bacterial/analysis/chemistry, DNA, Ribosomal/analysis, Fatty Acids, Fresh Water/microbiology, Hydrogen-Ion Concentration, Lipase, Molecular Sequence Data, Peptidoglycan, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Temperature, Vitamin K, *Water Microbiology
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitle
5844Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15322)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15322
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37394Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5237
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77041Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100510.1StrainInfo: A central database for resolving microbial strain identifiers
119385Curators of the CIPCollection of Institut Pasteur (CIP 107766)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107766