Strain identifier

BacDive ID: 7590

Type strain: Yes

Species: Arthrobacter roseus

Strain history: CIP <- 2002, S. Shivaji, CCMB, Hyderabad, India: strain CMS 90r

NCBI tax ID(s): 136274 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5274

BacDive-ID: 7590

DSM-Number: 14508

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic, Gram-positive

description: Arthrobacter roseus DSM 14508 is a psychrophilic, Gram-positive bacterium that was isolated from cyanobacterial mat sample.

NCBI tax id

  • NCBI tax id: 136274
  • Matching level: species

strain history

@refhistory
5274<- S. Shiva; CMS 90or
397792002, S. Shivaji, Hyderabad, India: strain CMS 90r
67770DSM 14508 <-- S. Shivaji CMS 90r.
122262CIP <- 2002, S. Shivaji, CCMB, Hyderabad, India: strain CMS 90r

doi: 10.13145/bacdive7590.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter roseus
  • full scientific name: Arthrobacter roseus Reddy et al. 2002

@ref: 5274

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter roseus

full scientific name: Arthrobacter roseus Reddy et al. 2002 emend. Busse 2016

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
122262positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18294Salmon orange (2012)10-14 daysISP 2
18294Salmon orange (2012)10-14 daysISP 3
18294Salmon orange (2012)10-14 daysISP 4
18294Salmon orange (2012)10-14 daysISP 5
18294Salmon orange (2012)10-14 daysISP 6
18294Salmon orange (2012)10-14 daysISP 7
122262

multicellular morphology

@refforms multicellular complexmedium name
18294noISP 2
18294noISP 3
18294noISP 4
18294noISP 5
18294noISP 6
18294noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5274TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18294ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18294ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18294ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18294ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18294ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18294ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39779MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122262CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18294positiveoptimum22psychrophilic
5274positivegrowth22psychrophilic
39779positivegrowth22psychrophilic
67770positivegrowth22psychrophilic
122262positivegrowth10-25psychrophilic
122262nogrowth30mesophilic
122262nogrowth37mesophilic
122262nogrowth41thermophilic
122262nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.369

halophily

@refsaltgrowthtested relationconcentration
122262NaClpositivegrowth0 %
122262NaClnogrowth2 %
122262NaClnogrowth4 %
122262NaClnogrowth6 %
122262NaClnogrowth8 %
122262NaClnogrowth10 %

murein

  • @ref: 5274
  • murein short key: A11.41
  • type: A3alpha L-Lys-Gly-L-Ala3

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1829462968cellulose-
1829416634raffinose-
1829426546rhamnose+
1829428757fructose+
1829429864mannitol-
1829417268myo-inositol-
1829418222xylose-
1829417992sucrose+
1829422599arabinose-
1829417234glucose+
12226216947citrate-carbon source
1222624853esculin-hydrolysis
122262606565hippurate+hydrolysis
12226217632nitrate+reduction
12226216301nitrite-reduction
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 122262
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122262
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 122262
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrazinamidase-3.5.1.B15
122262oxidase-
122262beta-galactosidase-3.2.1.23
122262alcohol dehydrogenase-1.1.1.1
122262gelatinase+/-
122262amylase+
122262DNase-
122262caseinase+3.4.21.50
122262catalase+1.11.1.6
122262tween esterase-
122262gamma-glutamyltransferase-2.3.2.2
122262lecithinase-
122262lipase-
122262lysine decarboxylase-4.1.1.18
122262ornithine decarboxylase-4.1.1.17
122262phenylalanine ammonia-lyase-4.3.1.24
122262tryptophan deaminase-
122262urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18294--+/-+/---+----------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18294+++-+--+-+----+----
122262-++-+----++---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.countryisolation date
5274cyanobacterial mat sampleAntarctica, McMurdo, Wright valley, pond L4Australia and Oceania
67770Cyanobacterial matMcMurdo Dry ValleysAntarcticaAntarcticaATA
122262Sample, cyanobacterial materialAntarcticaAntarcticaATA1995

isolation source categories

Cat1Cat2Cat3
#Environmental#Microbial community#Microbial mat
#Host#Microbial#Bacteria

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
182941Hazard group
52741Risk group (German classification)
1222621Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5274
  • description: Arthrobacter roseus partial 16S rRNA gene, strain CMS90
  • accession: AJ278870
  • length: 1477
  • database: ena
  • NCBI tax ID: 136274

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter roseus DSM 14508GCA_016907875contigncbi136274
66792Arthrobacter roseus strain DSM 14508136274.3wgspatric136274
66792Arthrobacter roseus DSM 145082893696664draftimg136274

GC content

  • @ref: 5274
  • GC-content: 66
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno54.128no
gram-positiveyes85.024no
anaerobicno99.498no
halophileno55.048no
spore-formingno92.561no
thermophileno97.812yes
glucose-utilyes83.29yes
flagellatedno90.644no
aerobicyes91.37no
glucose-fermentno91.312yes

External links

@ref: 5274

culture collection no.: DSM 14508, JCM 11881, MTCC 3712, CMS 90or, CIP 107726, NCIMB 14039, VTT E-072994, CMS 90r

straininfo link

  • @ref: 77034
  • straininfo: 265582

literature

  • topic: Phylogeny
  • Pubmed-ID: 12054218
  • title: Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.
  • authors: Reddy GSN, Prakash JSS, Matsumoto GI, Stackebrandt E, Shivaji S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-3-1017
  • year: 2002
  • mesh: Antarctic Regions, Arthrobacter/*chemistry/*classification/genetics, Bacterial Typing Techniques, Biomass, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, *Temperature
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
5274Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14508)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14508
18294Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM14508.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39779Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5193
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77034Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265582.1StrainInfo: A central database for resolving microbial strain identifiers
122262Curators of the CIPCollection of Institut Pasteur (CIP 107726)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107726