Strain identifier

BacDive ID: 7585

Type strain: Yes

Species: Arthrobacter luteolus

Strain Designation: CF25

Strain history: CIP <- 2013, DSMZ <- G. Wauters, Belgium: strain CF25 <- J. Wautelet

NCBI tax ID(s): 1216973 (strain), 98672 (species)

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General

@ref: 4894

BacDive-ID: 7585

DSM-Number: 13067

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile, rod-shaped

description: Arthrobacter luteolus CF25 is a mesophilic, motile, rod-shaped bacterium that was isolated from human surgical wound.

NCBI tax id

NCBI tax idMatching level
98672species
1216973strain

strain history

@refhistory
4894<- G. Wauters; CF25 <- J. Wautelet
67770CCUG 43811 <-- DSM 13067 <-- G. Wauters CF25 <-- J. Wautelet.
120695CIP <- 2013, DSMZ <- G. Wauters, Belgium: strain CF25 <- J. Wautelet

doi: 10.13145/bacdive7585.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter luteolus
  • full scientific name: Arthrobacter luteolus Wauters et al. 2000

@ref: 4894

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter luteolus

full scientific name: Arthrobacter luteolus Wauters et al. 2000

strain designation: CF25

type strain: yes

Morphology

cell morphology

  • @ref: 120695
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorincubation periodmedium used
18506Sulfur yellow (1016)10-14 daysISP 2
18506Sulfur yellow (1016)10-14 daysISP 3
18506Colorless10-14 daysISP 4
18506Sulfur yellow (1016)10-14 daysISP 5
18506Sulfur yellow (1016)10-14 daysISP 6
18506Sulfur yellow (1016)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18506noISP 2
18506noISP 3
18506noISP 4
18506noISP 5
18506noISP 6
18506noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4894TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18506ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18506ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18506ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18506ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18506ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18506ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33589MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120695CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18506positiveoptimum28mesophilic
4894positivegrowth30-37mesophilic
33589positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

murein

  • @ref: 4894
  • murein short key: A11.27
  • type: A3alpha L-Lys-L-Thr-L-Ala2

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18506-+-+--+-+++------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18506+++-+++++++---+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4894human surgical woundBelgiumBELEurope
67770Human surgical wound
120695Human, Surgical woundBelgiumBELEurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Wound
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_756.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_458;97_525;98_603;99_756&stattab=map
  • Last taxonomy: Arthrobacter
  • 16S sequence: AJ243422
  • Sequence Identity:
  • Total samples: 138
  • soil counts: 47
  • aquatic counts: 12
  • animal counts: 67
  • plant counts: 12

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
185062
48942Risk group (German classification)
1206951Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4894
  • description: Arthrobacter luteolus partial 16S rRNA gene
  • accession: AJ243422
  • length: 1499
  • database: ena
  • NCBI tax ID: 98672

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter luteolus NBRC 1078411216973.3wgspatric1216973
66792Arthrobacter luteolus strain DSM 1306798672.5wgspatric98672
66792Arthrobacter luteolus NBRC 1078412731957569draftimg1216973
66792Arthrobacter luteolus DSM 130672903042205draftimg98672
67770Arthrobacter luteolus NBRC 107841GCA_001552075contigncbi1216973
67770Arthrobacter luteolus DSM 13067GCA_008973725contigncbi98672

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno51.481no
gram-positiveyes90.319no
anaerobicno99.144no
aerobicyes91.292no
halophileno67.025no
spore-formingno89.238no
thermophileno99.21no
glucose-utilyes86.937no
flagellatedno93.115no
glucose-fermentno87.16no

External links

@ref: 4894

culture collection no.: DSM 13067, ATCC BAA 272, CCUG 43811, CIP 110642, JCM 11676, CCM 4907, CCUG 44605, CIP 106789, KCTC 9907, NBRC 107841

straininfo link

  • @ref: 77029
  • straininfo: 49403

literature

  • topic: Phylogeny
  • Pubmed-ID: 10835019
  • title: Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., isolated from human clinical specimens.
  • authors: Wauters G, Charlier J, Janssens M, Delmee M
  • journal: J Clin Microbiol
  • DOI: 10.1128/JCM.38.6.2412-2415.2000
  • year: 2000
  • mesh: Arthrobacter/*classification, Bacterial Typing Techniques, Genes, rRNA, Gram-Positive Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitle
4894Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13067)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13067
18506Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM13067.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33589Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30446
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77029Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49403.1StrainInfo: A central database for resolving microbial strain identifiers
120695Curators of the CIPCollection of Institut Pasteur (CIP 110642)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110642