Strain identifier

BacDive ID: 7583

Type strain: Yes

Species: Pseudoglutamicibacter albus

Strain Designation: CF43, CF 43

Strain history: CIP <- 2000, G. Wauters, UCL, Brussels, Belgium: strain CF 43 <- F. de Boelpaepe

NCBI tax ID(s): 98671 (species)

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General

@ref: 4895

BacDive-ID: 7583

DSM-Number: 13068

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Pseudoglutamicibacter albus CF43 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from human blood.

NCBI tax id

  • NCBI tax id: 98671
  • Matching level: species

strain history

@refhistory
4895<- G. Wauters; CF43 <- F. de Boelpaede
67770CIP 106791 <-- G. Wauters CF43 <-- F. de Boelpaepe.
116202CIP <- 2000, G. Wauters, UCL, Brussels, Belgium: strain CF 43 <- F. de Boelpaepe

doi: 10.13145/bacdive7583.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Pseudoglutamicibacter
  • species: Pseudoglutamicibacter albus
  • full scientific name: Pseudoglutamicibacter albus (Wauters et al. 2000) Busse 2016

  • synonyms

    • @ref: 20215
    • synonym: Arthrobacter albus

@ref: 4895

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Pseudoglutamicibacter

species: Pseudoglutamicibacter albus

full scientific name: Pseudoglutamicibacter albus (Wauters et al. 2000) Busse 2016

strain designation: CF43, CF 43

type strain: yes

Morphology

cell morphology

  • @ref: 116202
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18507Light ivory (1015)10-14 daysISP 2
18507Colorless10-14 daysISP 3
18507Colorless10-14 daysISP 4
18507Colorless10-14 daysISP 5
18507Colorless10-14 daysISP 6
18507Colorless10-14 daysISP 7
116202

multicellular morphology

@refforms multicellular complexmedium name
18507noISP 2
18507noISP 3
18507noISP 4
18507noISP 5
18507noISP 6
18507noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4895TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18507ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18507ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18507ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18507ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18507ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18507ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
41922MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116202CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
18507positiveoptimum28mesophilic
4895positivegrowth30-37mesophilic
41922positivegrowth37mesophilic
56042positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
116202positivegrowth25-37mesophilic
116202nogrowth10psychrophilic
116202nogrowth15psychrophilic
116202nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56042aerobe
116202obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
116202NaClpositivegrowth0-4 %
116202NaClnogrowth6 %
116202NaClnogrowth8 %
116202NaClnogrowth10 %

murein

  • @ref: 4895
  • murein short key: A11.35
  • type: A4alpha L-Lys-L-Ala-L-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11620216947citrate-carbon source
1162024853esculin-hydrolysis
116202606565hippurate+hydrolysis
11620217632nitrate-reduction
11620216301nitrite-reduction
11620217632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 116202
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116202
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11620215688acetoin-
11620217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382trypsin+3.4.21.4
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
116202oxidase+
116202beta-galactosidase-3.2.1.23
116202alcohol dehydrogenase-1.1.1.1
116202gelatinase-
116202amylase+
116202DNase+
116202caseinase-3.4.21.50
116202catalase+1.11.1.6
116202tween esterase-
116202gamma-glutamyltransferase+2.3.2.2
116202lecithinase-
116202lipase-
116202lysine decarboxylase-4.1.1.18
116202ornithine decarboxylase-4.1.1.17
116202phenylalanine ammonia-lyase-4.3.1.24
116202tryptophan deaminase-
116202urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18507-++------+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18507+-+-+++++++--------
116202-++-+--+-+----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116202------------+-----+--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
4895human bloodBelgiumBELEurope
56042Human blood,isolated in several blood culturesBelgiumBELEurope1984
67770Human blood
116202Human, BloodBelgiumBELEurope1984

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_4058.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2056;97_2481;98_3072;99_4058&stattab=map
  • Last taxonomy: Pseudoglutamicibacter albus
  • 16S sequence: AJ243421
  • Sequence Identity:
  • Total samples: 12536
  • soil counts: 244
  • aquatic counts: 505
  • animal counts: 11706
  • plant counts: 81

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
185072
48952Risk group (German classification)
1162021Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4895
  • description: Arthrobacter albus partial 16S rRNA gene
  • accession: AJ243421
  • length: 1501
  • database: ena
  • NCBI tax ID: 98671

Genome sequences

  • @ref: 66792
  • description: Pseudoglutamicibacter albus DSM 13068
  • accession: 2923410811
  • assembly level: draft
  • database: img
  • NCBI tax ID: 98671

External links

@ref: 4895

culture collection no.: DSM 13068, ATCC BAA 273, CCUG 43812, JCM 11943, CCM 4905, CCUG 44606, CIP 106791, KCTC 9908

straininfo link

  • @ref: 77027
  • straininfo: 49404

literature

  • topic: Phylogeny
  • Pubmed-ID: 10835019
  • title: Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., isolated from human clinical specimens.
  • authors: Wauters G, Charlier J, Janssens M, Delmee M
  • journal: J Clin Microbiol
  • DOI: 10.1128/JCM.38.6.2412-2415.2000
  • year: 2000
  • mesh: Arthrobacter/*classification, Bacterial Typing Techniques, Genes, rRNA, Gram-Positive Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4895Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13068)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13068
18507Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM13068.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41922Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18960
56042Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43812)https://www.ccug.se/strain?id=43812
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77027Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49404.1StrainInfo: A central database for resolving microbial strain identifiers
116202Curators of the CIPCollection of Institut Pasteur (CIP 106791)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106791