Strain identifier

BacDive ID: 7536

Type strain: Yes

Species: Arthrobacter ramosus

Strain Designation: I Gm25

Strain history: CIP <- 1986, DSM <- CCM <- MRI <- V. Jensen: strain IGm25 Central Exp. Farm, Ottawa, Canada

NCBI tax ID(s): 1672 (species)

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General

@ref: 6315

BacDive-ID: 7536

DSM-Number: 16186

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Arthrobacter ramosus I Gm25 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from beech forest soil.

NCBI tax id

  • NCBI tax id: 1672
  • Matching level: species

strain history

@refhistory
6315<- CCM <- MRI <- V. Jensen; I Gm25
67770IAM 12344 <-- CCM 1646 <-- MRI.
122136CIP <- 1986, DSM <- CCM <- MRI <- V. Jensen: strain IGm25 Central Exp. Farm, Ottawa, Canada

doi: 10.13145/bacdive7536.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter ramosus
  • full scientific name: Arthrobacter ramosus Jensen 1960 (Approved Lists 1980)

@ref: 6315

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter ramosus

full scientific name: Arthrobacter ramosus Jensen 1960

strain designation: I Gm25

type strain: yes

Morphology

cell morphology

  • @ref: 122136
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37395MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
6315TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
122136CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
122136CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
6315positivegrowth28mesophilic
37395positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122136
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12213617632nitrate-reduction
12213616301nitrite-reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 122136
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
122136oxidase-
122136catalase+1.11.1.6
122136urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47857C16:02.816
    47857C15:0 ANTEISO75.814.711
    47857C15:0 ISO3.814.621
    47857C16:0 iso3.215.626
    47857C17:0 anteiso14.516.722
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
47857--+++++-+----------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122136--+--+--+--+-+-+--+-

Isolation, sampling and environmental information

isolation

@refsample type
6315beech forest soil
47857Beech forest soil
67770Beech forest soil
122136Environment, Soil, beech forest

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Host#Plants#Tree

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63151Risk group (German classification)
1221361Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arthrobacter sp. DSM 20546 16S rRNA gene, strain DSM 20546X807421473ena1483557
6315Arthrobacter ramosus partial 16S rRNA gene, strain CCM 1646TAM0394351363ena1672

GC content

@refGC-contentmethod
6777062.2thermal denaturation, midpoint method (Tm)
6777063.1Buoyant density centrifugation (BD)

External links

@ref: 6315

culture collection no.: DSM 16186, ATCC 13727, CCM 1646, DSM 20546, NCIB 9066, CCUG 23844, JCM 1334, BCRC 14856, CIP 102361, IAM 12344, IFO 12672, IFO 12958, IMET 10685, KCTC 3386, LMG 17309, NBRC 12672, NBRC 12958, NCIMB 9066, NRRL B-3159, VKM Ac-1117, VKM Ac-1129

straininfo link

  • @ref: 76981
  • straininfo: 9073

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12508874Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.Altenburger P, Kampfer P, Schumann P, Steiner R, Lubitz W, Busse HJInt J Syst Evol Microbiol10.1099/00207713-52-6-20952002Austria, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Environmental Microbiology, Lipids/analysis, Micrococcaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Paintings, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicGenetics
Phylogeny24132914Arthrobacter gyeryongensis sp. nov., isolated from soil of a Gynostemma pentaphyllum field.Hoang VA, Kim YJ, Nguyen NL, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.053967-02013Arthrobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Gynostemma/*microbiology, Lysine/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics
Phylogeny24925596Arthrobacter bambusae sp. nov., isolated from soil of a bamboo grove.Park Y, Kook M, Ngo HTT, Kim KY, Park SY, Mavlonov GT, Yi THInt J Syst Evol Microbiol10.1099/ijs.0.064550-02014Arthrobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Bambusa/*microbiology, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny31079331Arthrobacter dokdonellae sp. nov., isolated from a plant of the genus Campanula.Koh HW, Kang MS, Lee KE, Lee EY, Kim H, Park SJJ Microbiol10.1007/s12275-019-8540-x2019Arthrobacter/classification/genetics/*isolation & purification/metabolism, Base Composition, Campanulaceae/*microbiology, Cell Wall/genetics/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Peptidoglycan/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of KoreaMetabolism
Phylogeny33555248Arthrobacter terricola sp. nov., isolated from forest soil.Trinh NH, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0046862021
Phylogeny35763420Arthrobacter cavernae sp. nov., a novel actinobacterium isolated from sediment of karst cave.Fang Z, Zhao X, Wu Q, Li S, Liu Q, Tan L, Weng QInt J Syst Evol Microbiol10.1099/ijsem.0.0054452022*Arthrobacter, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6315Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16186)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16186
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37395Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14039
47857Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 23844)https://www.ccug.se/strain?id=23844
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
76981Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9073.1StrainInfo: A central database for resolving microbial strain identifiers
122136Curators of the CIPCollection of Institut Pasteur (CIP 102361)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102361