Strain identifier

BacDive ID: 7533

Type strain: Yes

Species: Pseudarthrobacter polychromogenes

Strain Designation: 2568

Strain history: DSM 20136 <-- ATCC 15216 <-- M. P. Starr 2568 <-- A. F. Schippers-Lammertse.

NCBI tax ID(s): 1676 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8561

BacDive-ID: 7533

DSM-Number: 20136

keywords: 16S sequence, genome sequence, Bacteria, mesophilic

description: Pseudarthrobacter polychromogenes 2568 is a mesophilic bacterium that was isolated from air.

NCBI tax id

  • NCBI tax id: 1676
  • Matching level: species

strain history

doi: 10.13145/bacdive7533.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Pseudarthrobacter
  • species: Pseudarthrobacter polychromogenes
  • full scientific name: Pseudarthrobacter polychromogenes (Schippers-Lammertse et al. 1963) Busse 2016
  • synonyms

    • @ref: 20215
    • synonym: Arthrobacter polychromogenes

@ref: 8561

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Pseudarthrobacter

species: Pseudarthrobacter polychromogenes

full scientific name: Pseudarthrobacter polychromogenes (Schippers-Lammertse et al. 1963) Busse 2016

strain designation: 2568

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18627Concrete grey (7023)10-14 daysISP 2
18627Pebble grey (7032)10-14 daysISP 3
18627Slate grey (7015)10-14 daysISP 4
18627Light ivory (1015)10-14 daysISP 5
18627Sand yellow (1002)10-14 daysISP 6
18627Olive grey (7002)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18627noISP 2
18627noISP 3
18627noISP 4
18627noISP 5
18627noISP 6
18627noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18627ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18627ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18627ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18627ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18627ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18627ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
41905MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
8561CORYNEBACTERIUM AGAR (DSMZ Medium 53)yesName: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://bacmedia.dsmz.de/medium/53

culture temp

@refgrowthtypetemperaturerange
18627positiveoptimum28mesophilic
8561positivegrowth30mesophilic
41905positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

compound production

  • @ref: 8561
  • compound: indochrom

halophily

  • @ref: 18627
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

murein

  • @ref: 8561
  • murein short key: A11.23
  • type: A3alpha L-Lys-L-Ser-L-Thr-L-Ala

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1862762968cellulose+
1862716634raffinose+
1862726546rhamnose+
1862728757fructose-
1862729864mannitol-
1862717268myo-inositol+
1862718222xylose+
1862717992sucrose+
1862722599arabinose+
1862717234glucose+
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose-fermentation
6837917306maltose+fermentation
6837916899D-mannitol+fermentation
6837965327D-xylose-fermentation
6837916988D-ribose+fermentation
6837917634D-glucose+fermentation
683795291gelatin+hydrolysis
6837916199urea+hydrolysis
683794853esculin-hydrolysis
6837917632nitrate+reduction
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

  • @ref: 68368
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18627+++-++++-++++-++-+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18627+++-++++-++++-++-+-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDGEL
18627+---------

Isolation, sampling and environmental information

isolation

@refsample type
8561air
67770Air

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186271Hazard group
85611Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8561
  • description: A.polychromogenes 16S rDNA
  • accession: X80741
  • length: 1472
  • database: ena
  • NCBI tax ID: 1676

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudarthrobacter polychromogenes CGMCC 1.1927GCA_014644495scaffoldncbi1676
66792Pseudarthrobacter polychromogenes strain CGMCC 1.19271676.6wgspatric1676

GC content

  • @ref: 67770
  • GC-content: 62.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 8561

culture collection no.: DSM 20136, ATCC 15216, NCIB 10267, JCM 2523, BCRC 12114, CCUG 23891, CGMCC 1.1927, CIP 106989, IAM 14590, IFO 15512, KCTC 3384, LMG 16679, LMG 3821, NBRC 15512, NCIMB 10267, NRIC 0155, VKM Ac-1955

straininfo link

@refpassport
20218http://www.straininfo.net/strains/4079
20218http://www.straininfo.net/strains/4075
20218http://www.straininfo.net/strains/164009

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054218Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.Reddy GSN, Prakash JSS, Matsumoto GI, Stackebrandt E, Shivaji SInt J Syst Evol Microbiol10.1099/00207713-52-3-10172002Antarctic Regions, Arthrobacter/*chemistry/*classification/genetics, Bacterial Typing Techniques, Biomass, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, *TemperatureGenetics
Phylogeny33048040Pseudarthrobacter psychrotolerans sp. nov., a cold-adapted bacterium isolated from Antarctic soil.Shin Y, Lee BH, Lee KE, Park WInt J Syst Evol Microbiol10.1099/ijsem.0.0045052020Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Micrococcaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivativesTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8561Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20136)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20136
18627Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20136.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41905Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19180
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym