Strain identifier

BacDive ID: 7471

Type strain: Yes

Species: Mycetocola reblochoni

Strain Designation: BRB-1L41, R-20377

Strain history: DSM 18580 <-- N. Bora and M. Goodfellow; Newcastle Univ., UK; R-20377 (=BRB-1L41).

NCBI tax ID(s): 331618 (species)

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General

@ref: 7538

BacDive-ID: 7471

DSM-Number: 18580

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Mycetocola reblochoni BRB-1L41 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from surface of Reblochon cheese .

NCBI tax id

  • NCBI tax id: 331618
  • Matching level: species

strain history

@refhistory
7538<- R. M. Kroppenstedt, DSMZ <- N. Bora and M. Goodfellow, Univ. Newcastle; R-20377
67770DSM 18580 <-- N. Bora and M. Goodfellow; Newcastle Univ., UK; R-20377 (=BRB-1L41).

doi: 10.13145/bacdive7471.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Mycetocola
  • species: Mycetocola reblochoni
  • full scientific name: Mycetocola reblochoni Bora et al. 2008

@ref: 7538

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Mycetocola

species: Mycetocola reblochoni

full scientific name: Mycetocola reblochoni Bora et al. 2008

strain designation: BRB-1L41, R-20377

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31759positive1.1-1.5 µm0.3 µmrod-shapedno
69480no94.311
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
18413Zinc yellow (1018)10-14 daysISP 2
18413Beige (1001)10-14 daysISP 3
18413Beige (1001)10-14 daysISP 4
18413Zinc yellow (1018)10-14 daysISP 5
18413Zinc yellow (1018)10-14 daysISP 6
18413Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18413noISP 2
18413noISP 3
18413noISP 4
18413noISP 5
18413noISP 6
18413noISP 7

pigmentation

  • @ref: 31759
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7538TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18413ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18413ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18413ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18413ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18413ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18413ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
7538positivegrowth28mesophilic
18413positiveoptimum28mesophilic
31759positivegrowth20-30
31759positiveoptimum20-30
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31759positivegrowth07-10alkaliphile
31759positiveoptimum07-10

Physiology and metabolism

oxygen tolerance

  • @ref: 31759
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
31759no
69481no100
69480no99.999

observation

@refobservation
31759aggregates in chains
67770quinones: MK-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3175937684mannose+carbon source
3175917814salicin+carbon source
3175917992sucrose+carbon source
3175927082trehalose+carbon source
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
31759catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18413-+---++-+----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18413-++-++---+---+++---

Isolation, sampling and environmental information

isolation

@refsample type
7538surface of Reblochon cheese (late stage of ripening)
67770Surface of Reblochon cheese at the late stage of ripening

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

Safety information

risk assessment

  • @ref: 7538
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7538
  • description: Mycetocola reblochoni strain LMG 22367 16S ribosomal RNA gene, partial sequence
  • accession: DQ062097
  • length: 1400
  • database: ena
  • NCBI tax ID: 331618

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycetocola reblochoni strain JCM 30549331618.3wgspatric331618
66792Mycetocola reblochoni JCM 305492866450942draftimg331618
67770Mycetocola reblochoni JCM 30549GCA_003667525contigncbi331618

GC content

@refGC-contentmethod
753870.0
3175970
6777070high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveyes94.779no
anaerobicno99.386no
halophileno61.558no
spore-formingno94.29no
glucose-utilyes87.618no
motileno95.652no
flagellatedno97.018yes
thermophileno99.63yes
aerobicyes91.057no
glucose-fermentno87.406yes

External links

@ref: 7538

culture collection no.: DSM 18580, JCM 30549, LMG 22367

straininfo link

  • @ref: 76916
  • straininfo: 93763

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19060041Mycetocola reblochoni sp. nov., isolated from the surface microbial flora of Reblochon cheese.Bora N, Vancanneyt M, Gelsomino R, Snauwaert C, Swings J, Jones AL, Ward AC, Chamba JF, Kroppenstedt RM, Schumann P, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.64201-02008Actinomycetales/chemistry/*classification/genetics/*physiology, Cheese/*microbiology, Electrophoresis, Polyacrylamide Gel, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity, Vitamin K 2/analysisEnzymology
Phylogeny23291895Mycetocola miduiensis sp. nov., a psychrotolerant bacterium isolated from Midui glacier.Zhu L, Liu Q, Liu H, Zhou Y, Xin Y, Dong XInt J Syst Evol Microbiol10.1099/ijs.0.047985-02013Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Ice Cover/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Tibet, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7538Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18580)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18580
18413Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM18580.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31759Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2803228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76916Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID93763.1StrainInfo: A central database for resolving microbial strain identifiers