Strain identifier

BacDive ID: 7470

Type strain: Yes

Species: Mycetocola tolaasinivorans

Strain Designation: CM-05

Strain history: <- NBRC <- T. Tsukamoto; CM-05

NCBI tax ID(s): 76635 (species)

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General

@ref: 5744

BacDive-ID: 7470

DSM-Number: 15179

keywords: 16S sequence, Bacteria, mesophilic

description: Mycetocola tolaasinivorans CM-05 is a mesophilic bacterium that was isolated from cultivated mushroom Pleurotus ostreatus.

NCBI tax id

  • NCBI tax id: 76635
  • Matching level: species

strain history

  • @ref: 5744
  • history: <- NBRC <- T. Tsukamoto; CM-05

doi: 10.13145/bacdive7470.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Mycetocola
  • species: Mycetocola tolaasinivorans
  • full scientific name: Mycetocola tolaasinivorans Tsukamoto et al. 2001

@ref: 5744

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Mycetocola

species: Mycetocola tolaasinivorans

full scientific name: Mycetocola tolaasinivorans Tsukamoto et al. 2001

strain designation: CM-05

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18390Beige (1001)10-14 daysISP 2
18390Beige (1001)10-14 daysISP 3
1839010-14 daysISP 4
1839010-14 daysISP 5
1839010-14 daysISP 6
1839010-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18390noISP 2
18390noISP 3
18390noISP 4
18390noISP 5
18390noISP 6
18390noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5744TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18390ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18390ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18390ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18390ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18390ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18390ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml

culture temp

@refgrowthtypetemperaturerange
18390positiveoptimum28mesophilic
5744positivegrowth28mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1839017234glucose-
1839022599arabinose-
1839017992sucrose+
1839018222xylose+
1839017268myo-inositol-
1839029864mannitol-
1839028757fructose-
1839026546rhamnose-
1839016634raffinose+
1839062968cellulose-
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917992sucrose-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELSAC
18390++--++-+---

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18390-++-++++---++-++---

Isolation, sampling and environmental information

isolation

  • @ref: 5744
  • sample type: cultivated mushroom Pleurotus ostreatus
  • host species: Pleurotus ostreatus
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Host
  • Cat2: #Fungi
  • Cat3: #Mushroom

taxonmaps

  • @ref: 69479
  • File name: preview.99_3087.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_417;97_1928;98_2371;99_3087&stattab=map
  • Last taxonomy: Microbacterium
  • 16S sequence: AB012646
  • Sequence Identity:
  • Total samples: 1043
  • soil counts: 136
  • aquatic counts: 298
  • animal counts: 351
  • plant counts: 258

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
57441Risk group (German classification)
183901German classification

Sequence information

16S sequences

  • @ref: 5744
  • description: Mycetocola tolaasinivorans gene for 16S rRNA
  • accession: AB012646
  • length: 1444
  • database: ena
  • NCBI tax ID: 76635

GC content

  • @ref: 5744
  • GC-content: 65.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5744

culture collection no.: DSM 15179, IFO 16277, NBRC 16277, NRRL B-24120

straininfo link

  • @ref: 76915
  • straininfo: 62366

literature

  • topic: Phylogeny
  • Pubmed-ID: 11411718
  • title: Proposal of Mycetocola gen. nov. in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp. nov., Mycetocola tolaasinivorans sp. nov. and Mycetocola lacteus sp. nov., isolated from cultivated mushroom, Pleurotus ostreatus.
  • authors: Tsukamoto T, Takeuchi M, Shida O, Murata H, Shirata A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-3-937
  • year: 2001
  • mesh: Actinomycetales/*classification/genetics/*physiology/ultrastructure, *Agaricales, DNA Primers, DNA, Ribosomal/genetics, Japan, Molecular Sequence Data, Peptidoglycan/chemistry/metabolism, *Phylogeny, RNA, Ribosomal, 16S/genetics, Vitamin K/metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
5744Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15179)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15179
18390Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM15179.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76915Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID62366.1StrainInfo: A central database for resolving microbial strain identifiers