Strain identifier

BacDive ID: 7470

Type strain: Yes

Species: Mycetocola tolaasinivorans

Strain Designation: CM-05

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General

@ref: 5744

BacDive-ID: 7470

DSM-Number: 15179

keywords: Bacteria, 16S sequence, genome sequence, mesophilic

description: Mycetocola tolaasinivorans CM-05 is a mesophilic bacterium that was isolated from cultivated mushroom Pleurotus ostreatus.

strain history: <- NBRC <- T. Tsukamoto; CM-05

doi: 10.13145/bacdive7470.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Mycetocola
  • species: Mycetocola tolaasinivorans
  • full scientific name: Mycetocola tolaasinivorans Tsukamoto et al. 2001

@ref: 5744

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Mycetocola

species: Mycetocola tolaasinivorans

full scientific name: Mycetocola tolaasinivorans Tsukamoto et al. 2001

strain designation: CM-05

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18390Beige (1001)10-14 daysISP 2
18390Beige (1001)10-14 daysISP 3
1839010-14 daysISP 4
1839010-14 daysISP 5
1839010-14 daysISP 6
1839010-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18390noISP 2
18390noISP 3
18390noISP 4
18390noISP 5
18390noISP 6
18390noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5744TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
18390ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18390ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18390ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18390ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18390ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18390ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml

culture temp

@refgrowthtypetemperaturerange
18390positiveoptimum28mesophilic
5744positivegrowth28mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1839017234glucose-
1839022599arabinose-
1839017992sucrose+
1839018222xylose+
1839017268myo-inositol-
1839029864mannitol-
1839028757fructose-
1839026546rhamnose-
1839016634raffinose+
1839062968cellulose-

API coryne

@refPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELSAC
18390++--++-+---

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18390-++-++++---++-++---

Isolation, sampling and environmental information

isolation

  • @ref: 5744
  • sample type: cultivated mushroom Pleurotus ostreatus
  • host species: Pleurotus ostreatus
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Host
  • Cat2: #Fungi
  • Cat3: #Mushroom

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183901German classification
57441Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5744
  • description: Mycetocola tolaasinivorans gene for 16S rRNA
  • accession: AB012646
  • length: 1444
  • database: ena
  • NCBI tax ID: 76635

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycetocola tolaasinivorans IF 016277GCA_003667565contigncbi76635
66792Mycetocola tolaasinivorans strain IF 01627776635.4wgspatric76635

GC content

  • @ref: 5744
  • GC-content: 65.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5744

culture collection no.: DSM 15179, IFO 16277, NBRC 16277, NRRL B-24120

straininfo link

@refpassport
20218http://www.straininfo.net/strains/303117
20218http://www.straininfo.net/strains/221907
20218http://www.straininfo.net/strains/337348
20218http://www.straininfo.net/strains/303118

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5744Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15179)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15179
18390Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM15179.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)