Strain identifier

BacDive ID: 7452

Type strain: Yes

Species: Microbacterium azadirachtae

Strain Designation: AI-S262

Strain history: <- M Madhaiyan, Tamilnadu Agri. Univ., India

NCBI tax ID(s): 582680 (species)

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General

@ref: 17402

BacDive-ID: 7452

DSM-Number: 23848

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, motile, rod-shaped

description: Microbacterium azadirachtae AI-S262 is an aerobe, Gram-positive, motile bacterium that was isolated from rhizoplane of neem Azadirachta indica seedlings.

NCBI tax id

  • NCBI tax id: 582680
  • Matching level: species

strain history

@refhistory
17402<- JCM <- M. Madhaiyan, Tamil Nadu Agric. Univ., Coimbatore, India
67770M. Madhaiyan AI-S262.
67771<- M Madhaiyan, Tamilnadu Agri. Univ., India

doi: 10.13145/bacdive7452.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium azadirachtae
  • full scientific name: Microbacterium azadirachtae Madhaiyan et al. 2010

@ref: 17402

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium azadirachtae

full scientific name: Microbacterium azadirachtae Madhaiyan et al. 2010

strain designation: AI-S262

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
29556positive0.6-0.7 µm0.3-0.4 µmrod-shapedyes
67771positive

pigmentation

  • @ref: 29556
  • production: yes

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
29556positivegrowth10-37
29556positiveoptimum28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29556positivegrowth05-10alkaliphile
29556positiveoptimum07-08

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29556aerobe
67771aerobe

spore formation

  • @ref: 29556
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29556NaClpositivegrowth0-5.5 %
29556NaClpositiveoptimum0-1 %

observation

  • @ref: 67770
  • observation: quinones: MK-12, MK-13

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2955616449alanine+carbon source
2955622599arabinose+carbon source
2955616947citrate+carbon source
2955625115malate+carbon source
295564853esculin+hydrolysis

enzymes

  • @ref: 29556
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
17402rhizoplane of neem Azadirachta indica seedlingsAzadirachta indicaCoimbatore, Tamilnadu, Botanical GardenIndiaINDAsia
67770Rhizoplane of neem (Azadirachta indica) seedlings at Tamilnadu Agric. Univ.Azadirachta indicaCoimbatore, TamilnaduIndiaINDAsia
67771From Neem (`Azadirachta indica` A. Juss), rhizoplaneTamilnadu Agricultural University, Coimbatore, TaminaduIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Rhizoplane

Safety information

risk assessment

  • @ref: 17402
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17402
  • description: Microbacterium azadirachtae strain AI-S262 16S ribosomal RNA gene, partial sequence
  • accession: EU912487
  • length: 1373
  • database: ena
  • NCBI tax ID: 582680

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Microbacterium azadirachtae strain DSM 23848582680.7wgspatric582680
66792Microbacterium azadirachtae DSM 238482773857981draftimg582680
67770Microbacterium azadirachtae DSM 23848GCA_000956545contigncbi582680

GC content

@refGC-contentmethod
1740269.5high performance liquid chromatography (HPLC)
2955669.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes93.06yes
anaerobicno99.586no
halophileno94.723yes
spore-formingno91.982no
glucose-utilyes84.943no
thermophileno99.64yes
flagellatedno98.127no
motileno88.283no
aerobicyes93.714no
glucose-fermentno90.299no

External links

@ref: 17402

culture collection no.: DSM 23848, JCM 15681, KCTC 19668, LMG 24772

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19734284Microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings.Madhaiyan M, Poonguzhali S, Lee JS, Lee KC, Saravanan VS, Santhanakrishnan PInt J Syst Evol Microbiol10.1099/ijs.0.015800-02009Actinomycetales/*classification/genetics/isolation & purification/physiology, Azadirachta/*microbiology, Base Composition, Base Sequence, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Seedlings/*microbiologyGenetics
Phylogeny25563915Microbacterium panaciterrae sp. nov., isolated from the rhizosphere of ginseng.Nguyen NL, Kim YJ, Hoang VA, Min JW, Hwang KH, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.0000412015Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Panax/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
17402Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23848)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23848
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29556Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2595128776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc