Strain identifier

BacDive ID: 7441

Type strain: Yes

Species: Microbacterium fluvii

Strain Designation: YSL3-15

Strain history: DSM 18908 <-- H. Kasai YSL3-15.

NCBI tax ID(s): 415215 (species)

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General

@ref: 7768

BacDive-ID: 7441

DSM-Number: 18908

keywords: 16S sequence, Bacteria, mesophilic

description: Microbacterium fluvii YSL3-15 is a mesophilic bacterium that was isolated from driftwood.

NCBI tax id

  • NCBI tax id: 415215
  • Matching level: species

strain history

@refhistory
7768<- Hiroaki Kasai; YSL3-15
67770DSM 18908 <-- H. Kasai YSL3-15.

doi: 10.13145/bacdive7441.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium fluvii
  • full scientific name: Microbacterium fluvii Kageyama et al. 2008

@ref: 7768

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium fluvii

full scientific name: Microbacterium fluvii Kageyama et al. 2008

strain designation: YSL3-15

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 7768
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7768positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-11, MK-12

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
7768----+-+---++++-+--+----+++++++-++---+/---++------+/---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7768driftwoodMaera River, Iriomote IslandJapanJPNAsia
67770Driftwood at the estuary of the Maera RiverIriomote Island, Okinawa Pref.JapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Host Body Product#Plant#Timber
#Host#Plants#Tree

Safety information

risk assessment

  • @ref: 7768
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7768
  • description: Microbacterium fluvii gene for 16S rRNA, partial sequence
  • accession: AB286028
  • length: 1405
  • database: ena
  • NCBI tax ID: 415215

GC content

@refGC-contentmethod
776870
6777070high performance liquid chromatography (HPLC)

External links

@ref: 7768

culture collection no.: DSM 18908, JCM 16545, MBIC 08277, KACC 14369, NBRC 105003

straininfo link

  • @ref: 76889
  • straininfo: 404749

Reference

@idauthorscataloguedoi/urltitle
7768Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18908)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18908
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
76889Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404749.1StrainInfo: A central database for resolving microbial strain identifiers