Strain identifier

BacDive ID: 7412

Type strain: Yes

Species: Microbacterium aerolatum

Strain Designation: V-73

Strain history: CIP <- 2002, CCM <- C. Zlamala: strain V-73

NCBI tax ID(s): 153731 (species)

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General

@ref: 5256

BacDive-ID: 7412

DSM-Number: 14217

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive

description: Microbacterium aerolatum V-73 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from air.

NCBI tax id

  • NCBI tax id: 153731
  • Matching level: species

strain history

@refhistory
5256<- C. Zlamala; V-73
67770CCM 4955 <-- C. Zlamala V-73.
116072CIP <- 2002, CCM <- C. Zlamala: strain V-73

doi: 10.13145/bacdive7412.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium aerolatum
  • full scientific name: Microbacterium aerolatum Zlamala et al. 2002

@ref: 5256

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium aerolatum

full scientific name: Microbacterium aerolatum Zlamala et al. 2002

strain designation: V-73

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
116072positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18511Lemon yellow (1012)10-14 daysISP 2
18511Lemon yellow (1012)10-14 daysISP 3
18511Lemon yellow (1012)10-14 daysISP 4
18511Lemon yellow (1012)10-14 daysISP 5
18511Lemon yellow (1012)10-14 daysISP 6
18511Lemon yellow (1012)10-14 daysISP 7
116072

multicellular morphology

@refforms multicellular complexmedium name
18511noISP 2
18511noISP 3
18511noISP 4
18511noISP 5
18511noISP 6
18511noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5256TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18511ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18511ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18511ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18511ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18511ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18511ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38218MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116072CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18511positiveoptimum28mesophilic
5256positivegrowth28mesophilic
38218positivegrowth25mesophilic
67770positivegrowth28mesophilic
116072positivegrowth15-37
116072nogrowth10psychrophilic
116072nogrowth41thermophilic
116072nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116072
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.867

halophily

@refsaltgrowthtested relationconcentration
116072NaClpositivegrowth0-4 %
116072NaClnogrowth6 %
116072NaClnogrowth8 %
116072NaClnogrowth10 %

murein

  • @ref: 5256
  • murein short key: B06
  • type: B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

observation

  • @ref: 67770
  • observation: quinones: MK-12, MK-13

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1851162968cellulose+
1851116634raffinose-
1851126546rhamnose+
1851128757fructose+
1851129864mannitol+
1851117268myo-inositol-
1851118222xylose-
1851117992sucrose+
1851122599arabinose+
1851117234glucose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11607216947citrate-carbon source
1160724853esculin+hydrolysis
116072606565hippurate+hydrolysis
11607217632nitrate+reduction
11607216301nitrite-reduction
11607217632nitrate-respiration
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 116072
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116072
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11607215688acetoin-
11607217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116072oxidase-
116072beta-galactosidase+3.2.1.23
116072alcohol dehydrogenase-1.1.1.1
116072gelatinase-
116072amylase-
116072DNase+
116072caseinase-3.4.21.50
116072catalase+1.11.1.6
116072tween esterase+
116072gamma-glutamyltransferase-2.3.2.2
116072lecithinase-
116072lipase-
116072lysine decarboxylase-4.1.1.18
116072ornithine decarboxylase-4.1.1.17
116072phenylalanine ammonia-lyase-4.3.1.24
116072tryptophan deaminase-
116072urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18511+-----+-++-++--+-+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18511-++-++++-+---+++++-
116072-++-++-+-++---++++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116072---------+/-+/-+/-+/--+/---+/---+/----+/--+/-+/---+/---+/------+/----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116072+++++--+--+----+--+--------------+-+--------------------------+-----------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5256airWien, VirgilkapelleAustriaAUTEurope
67770Air in the chapel 'Virgikapelle'ViennaAustriaAUTEurope
116072Environment, Air, chapelViennaAustriaAUTEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52561Risk group (German classification)
1160721Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5256
  • description: Microbacterium aerolatum 16S rRNA gene
  • accession: AJ309929
  • length: 1402
  • database: ena
  • NCBI tax ID: 153731

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Microbacterium aerolatum NBRC 103071GCA_007988825contigncbi153731
66792Microbacterium aerolatum CCM 4955GCA_014635005scaffoldncbi153731
66792Microbacterium aerolatum strain CCM 4955153731.5wgspatric153731
66792Microbacterium aerolatum strain NBRC 103071153731.4wgspatric153731

GC content

  • @ref: 5256
  • GC-content: 69.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno91.96no
gram-positiveyes92.944no
anaerobicno99.557no
aerobicyes93.955no
halophileno80.225no
spore-formingno87.855no
thermophileno99.386no
glucose-utilyes86.265yes
motileyes70.115no
glucose-fermentno87.735no

External links

@ref: 5256

culture collection no.: DSM 14217, CCM 4955, NRRL B-24228, JCM 12137, CIP 107636, IAM 15195, LMG 22184, NBRC 103071

straininfo link

  • @ref: 76861
  • straininfo: 100774

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12148633Microbacterium aerolatum sp. nov., isolated from the air in the 'Virgilkapelle' in Vienna.Zlamala C, Schumann P, Kampfer P, Valens M, Rossello-Mora R, Lubitz W, Busse HJInt J Syst Evol Microbiol10.1099/00207713-52-4-12292002Actinomycetales/chemistry/*classification/*isolation & purification/physiology, *Air Microbiology, Austria, Bacterial Typing Techniques, DNA Fingerprinting, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Molecular Sequence Data, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Spectroscopy, Fourier Transform InfraredGenetics
Phylogeny20081025Microbacterium ginsengiterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.Kim YJ, Kim MK, Bui TPN, Kim HB, Srinivasan S, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.015784-02010Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Panax/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry, beta-Glucosidase/metabolismGenetics
Phylogeny25563915Microbacterium panaciterrae sp. nov., isolated from the rhizosphere of ginseng.Nguyen NL, Kim YJ, Hoang VA, Min JW, Hwang KH, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.0000412015Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Panax/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics
Phylogeny25678679Microbacterium shaanxiense sp. nov., isolated from the nodule surface of soybean.Peng S, Dongying L, Bingxin Y, Mingjun L, Gehong WInt J Syst Evol Microbiol10.1099/ijs.0.0001162015Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Soybeans/*microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5256Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14217)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14217
18511Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM14217.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38218Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5093
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76861Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100774.1StrainInfo: A central database for resolving microbial strain identifiers
116072Curators of the CIPCollection of Institut Pasteur (CIP 107636)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107636