Strain identifier
BacDive ID: 7404
Type strain:
Species: Microbacterium aurantiacum
Strain history: CIP <- 1998, IFO <- NCFB <- M.D. Collins, Leicester Univ. <- Dias & Bhat
NCBI tax ID(s): 162393 (species)
General
@ref: 4752
BacDive-ID: 7404
DSM-Number: 12506
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped
description: Microbacterium aurantiacum DSM 12506 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from sewage.
NCBI tax id
- NCBI tax id: 162393
- Matching level: species
strain history
@ref | history |
---|---|
4752 | <- IFO <- NCFB (Microbacterium laevaniformans) <- M.D. Collins, C820 <- Dias & Bhat, 8-3-56 (Corynebacterium laevaniformans) |
67770 | IFO 15234 <-- NCFB 2288 (Microbacterium laevaniformans) <-- M. D. Collins C820 <-- F. F. Dias and J. V. Bhat. |
116558 | CIP <- 1998, IFO <- NCFB <- M.D. Collins, Leicester Univ. <- Dias & Bhat |
doi: 10.13145/bacdive7404.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Microbacteriaceae
- genus: Microbacterium
- species: Microbacterium aurantiacum
- full scientific name: Microbacterium aurantiacum Takeuchi and Hatano 1998
@ref: 4752
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Microbacteriaceae
genus: Microbacterium
species: Microbacterium aurantiacum
full scientific name: Microbacterium aurantiacum Takeuchi and Hatano 1998
type strain: yes
Morphology
cell morphology
- @ref: 116558
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18364 | Melon yellow (1028) | 10-14 days | ISP 2 |
18364 | Melon yellow (1028) | 10-14 days | ISP 3 |
18364 | Melon yellow (1028) | 10-14 days | ISP 4 |
18364 | Melon yellow (1028) | 10-14 days | ISP 5 |
18364 | Melon yellow (1028) | 10-14 days | ISP 6 |
18364 | Melon yellow (1028) | 10-14 days | ISP 7 |
116558 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
18364 | no | ISP 2 |
18364 | no | ISP 3 |
18364 | no | ISP 4 |
18364 | no | ISP 5 |
18364 | no | ISP 6 |
18364 | no | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4752 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
18364 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
18364 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18364 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18364 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18364 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
18364 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
39348 | MEDIUM 119 - for Azotobacter vinelandii | yes | Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (15.000 g);Yeast extract (2.000 g);Peptone (5.000 g);Tryptone (5.000 g) | |
116558 | CIP Medium 1 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
18364 | positive | optimum | 28 | mesophilic |
4752 | positive | growth | 30 | mesophilic |
39348 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
116558 | positive | growth | 15-37 | |
116558 | no | growth | 10 | psychrophilic |
116558 | no | growth | 41 | thermophilic |
116558 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116558
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
116558 | NaCl | positive | growth | 0-8 % |
116558 | NaCl | no | growth | 10 % |
murein
- @ref: 4752
- murein short key: B06
- type: B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn
observation
- @ref: 67770
- observation: quinones: MK-12
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | + | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | + | builds acid from | 28087 |
68371 | starch | + | builds acid from | 28017 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | + | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | + | builds acid from | 65327 |
68371 | D-ribose | - | builds acid from | 16988 |
68371 | L-arabinose | + | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
116558 | citrate | - | carbon source | 16947 |
116558 | esculin | + | hydrolysis | 4853 |
116558 | hippurate | + | hydrolysis | 606565 |
116558 | nitrate | - | reduction | 17632 |
116558 | nitrite | - | reduction | 16301 |
116558 | nitrate | - | respiration | 17632 |
metabolite production
- @ref: 116558
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
116558 | 15688 | acetoin | - | |
116558 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
116558 | oxidase | - | |
116558 | beta-galactosidase | + | 3.2.1.23 |
116558 | alcohol dehydrogenase | - | 1.1.1.1 |
116558 | gelatinase | +/- | |
116558 | amylase | + | |
116558 | DNase | - | |
116558 | caseinase | - | 3.4.21.50 |
116558 | catalase | + | 1.11.1.6 |
116558 | tween esterase | - | |
116558 | gamma-glutamyltransferase | - | 2.3.2.2 |
116558 | lecithinase | - | |
116558 | lipase | - | |
116558 | lysine decarboxylase | - | 4.1.1.18 |
116558 | ornithine decarboxylase | - | 4.1.1.17 |
116558 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
116558 | protease | - | |
116558 | tryptophan deaminase | - | |
116558 | urease | - | 3.5.1.5 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18364 | + | - | + | - | + | + | + | + | + | + | + | + | + | + | + | + | - | - | - | |
116558 | + | + | + | - | + | - | - | - | - | + | + | + | + | - | + | + | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116558 | + | - | - | + | - | + | - | - | +/- | + | + | + | + | - | - | - | - | +/- | - | - | - | - | +/- | +/- | +/- | + | + | + | - | - | + | + | - | + | + | + | + | - | - | + | - | - | - | - | - | - | - | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116558 | + | + | + | + | + | - | - | + | + | + | + | - | - | - | - | + | - | - | + | - | - | + | + | - | - | + | - | - | - | - | - | + | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | + | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
4752 | sewage |
67770 | Sewage |
116558 | Environment, Sewage |
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Wastewater
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4752 | 1 | Risk group (German classification) |
116558 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Microbacterium aurantiacum partial 16S rRNA gene, type strain DSM 12506 | AM182159 | 1356 | ena | 162393 |
4752 | Microbacterium aurantiacum gene for 16S rRNA, partial sequence | AB004726 | 1482 | ena | 162393 |
GC content
@ref | GC-content | method |
---|---|---|
4752 | 70.3 | |
67770 | 70.3 | high performance liquid chromatography (HPLC) |
External links
@ref: 4752
culture collection no.: DSM 12506, ATCC 49090, IFO 15234, NBRC 15234, NCFB 2288, NRRL B-24217, JCM 9177, CIP 105730, IAM 15208, NCIMB 702288, VKM Ac-2075
straininfo link
- @ref: 76853
- straininfo: 42284
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 9734054 | Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. | Takeuchi M, Hatano K | Int J Syst Bacteriol | 10.1099/00207713-48-3-973 | 1998 | Actinomycetales/chemistry/*classification/genetics, DNA, Bacterial/analysis, Flavobacterium/*classification, Nucleic Acid Hybridization | |
Phylogeny | 29458489 | Microbacterium telephonicum sp. nov., isolated from the screen of a cellular phone. | Rahi P, Kurli R, Pansare AN, Khairnar M, Jagtap S, Patel NB, Dastager SG, Lawson PA, Shouche YS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002622 | 2018 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Cell Phone, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4752 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12506) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12506 | |||
18364 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM12506.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
39348 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17782 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
76853 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42284.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116558 | Curators of the CIP | Collection of Institut Pasteur (CIP 105730) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105730 |