Strain identifier

BacDive ID: 7376

Type strain: Yes

Species: Microbacterium luteolum

Strain Designation: 69

Strain history: CIP <- 1985, DSM <- NCIB <- T. Gibson <- L.E. Topping: strain 69

NCBI tax ID(s): 69367 (species)

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General

@ref: 8567

BacDive-ID: 7376

DSM-Number: 20143

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Microbacterium luteolum 69 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 69367
  • Matching level: species

strain history

@refhistory
8567<- NCIB <- T. Gibson <- L.E. Topping, 69 (coryneform organism, group 2a)
67770IFO 15074 <-- DSM 20143 <-- NCIMB 9568 <-- T. Gibson <-- L. E. Topping No. 69 (group 2a).
120693CIP <- 1985, DSM <- NCIB <- T. Gibson <- L.E. Topping: strain 69

doi: 10.13145/bacdive7376.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium luteolum
  • full scientific name: Microbacterium luteolum (Yokota et al. 1993) Takeuchi and Hatano 1998

  • synonyms

    • @ref: 20215
    • synonym: Aureobacterium luteolum

@ref: 8567

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium luteolum

full scientific name: Microbacterium luteolum (Yokota et al. 1993) Takeuchi and Hatano 1998

strain designation: 69

type strain: yes

Morphology

cell morphology

  • @ref: 120693
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18424Colorless10-14 daysISP 2
18424Zinc yellow (1018)10-14 daysISP 3
18424Beige (1001)10-14 daysISP 4
18424Beige (1001)10-14 daysISP 5
18424Zinc yellow (1018)10-14 daysISP 6
18424Colorless10-14 daysISP 7
120693

multicellular morphology

@refforms multicellular complexmedium name
18424noISP 2
18424noISP 3
18424noISP 4
18424noISP 5
18424noISP 6
18424noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8567CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://mediadive.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18424ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18424ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18424ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18424ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18424ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18424ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40319MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120693CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18424positiveoptimum28mesophilic
8567positivegrowth30mesophilic
40319positivegrowth30mesophilic
67770positivegrowth28mesophilic
120693positivegrowth15-30
120693nogrowth10psychrophilic
120693nogrowth37mesophilic
120693nogrowth41thermophilic
120693nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120693
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120693NaClpositivegrowth0-4 %
120693NaClnogrowth6 %
120693NaClnogrowth8 %
120693NaClnogrowth10 %

murein

  • @ref: 8567
  • murein short key: B06
  • type: B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

observation

  • @ref: 67770
  • observation: quinones: MK-12, MK-11, MK-13

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12069316947citrate-carbon source
1206934853esculin+hydrolysis
120693606565hippurate+hydrolysis
12069317632nitrate-reduction
12069316301nitrite-reduction
12069317632nitrate-respiration
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 120693
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120693
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12069315688acetoin-
12069317234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-chymotrypsin-3.4.21.1
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
120693oxidase-
120693beta-galactosidase+3.2.1.23
120693alcohol dehydrogenase-1.1.1.1
120693gelatinase+/-
120693amylase-
120693DNase+
120693caseinase+3.4.21.50
120693catalase+1.11.1.6
120693tween esterase-
120693gamma-glutamyltransferase-2.3.2.2
120693lecithinase-
120693lipase-
120693lysine decarboxylase-4.1.1.18
120693ornithine decarboxylase-4.1.1.17
120693phenylalanine ammonia-lyase-4.3.1.24
120693protease-
120693tryptophan deaminase-
120693urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18424-----++-+++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18424-++-++------+-++-+-
120693-++-++++-++++-++-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120693+++++--++++----+--+-+++--+-----+-+-+---+-----------------+-++-------------------------+----------+-

Isolation, sampling and environmental information

isolation

@refsample type
8567soil
67770Soil
120693Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85671Risk group (German classification)
184241German classification
1206931Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Microbacterium luteolum 16S rRNA geneY172351462ena69367
20218Microbacterium luteolum gene for 16S rRNA, partial sequenceAB0047181440ena69367

GC content

@refGC-contentmethod
856771.0high performance liquid chromatography (HPLC)
856775.6
6777070.6high performance liquid chromatography (HPLC)

External links

@ref: 8567

culture collection no.: DSM 20143, ATCC 51474, IFO 15074, NBRC 15074, NCIB 9568, NRRL B-24230, JCM 9174, CCM 4365, CCUG 43281, CIP 102086, IAM 15204, LMG 16207, NCIMB 9568, VTT E-97042

straininfo link

  • @ref: 76825
  • straininfo: 8204

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8347513Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.Yokota A, Takeuchi M, Sakane T, Weiss NInt J Syst Bacteriol10.1099/00207713-43-3-5551993Bacterial Typing Techniques, Base Composition, Carbohydrate Sequence, DNA, Bacterial/chemistry, Fatty Acids/analysis, Flavobacterium/chemistry/*classification/cytology, Gram-Positive Asporogenous Rods/chemistry/*classification/cytology, Molecular Sequence Data, Mycolic Acids/analysis, Peptidoglycan/chemistry, Vitamin K/analysisGenetics
Phylogeny15774639Microbacterium oleivorans sp. nov. and Microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading Gram-positive bacteria.Schippers A, Bosecker K, Sproer C, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.63305-02005Actinomycetales/*classification/genetics/metabolism/ultrastructure, Bacterial Typing Techniques, Biodegradation, Environmental, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Gram-Positive Bacteria/classification/metabolism, Molecular Sequence Data, Petroleum/*metabolism, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityMetabolism
Enzymology20159566Gene cloning and characterization of dihydrolipoamide dehydrogenase from Microbacterium luteolum: A useful enzymatic regeneration system of NAD+ from NADH.Kurokawa J, Asano M, Nomoto S, Makino Y, Itoh NJ Biosci Bioeng10.1016/j.jbiosc.2009.09.0402010Cloning, Molecular/methods, Dihydrolipoamide Dehydrogenase/*chemistry/genetics/*metabolism, Enzyme Activation, Enzyme Stability, Escherichia coli/*enzymology/genetics, Micrococcaceae/*enzymology/genetics, NAD/*metabolism, Protein Engineering/*methodsMetabolism
Enzymology23202966Production of (R)-3-quinuclidinol by E. coli biocatalysts possessing NADH-dependent 3-quinuclidinone reductase (QNR or bacC) from Microbacterium luteolum and Leifsonia alcohol dehydrogenase (LSADH).Isotani K, Kurokawa J, Itoh NInt J Mol Sci10.3390/ijms1310135422012Actinomycetales/*enzymology, Alcohol Dehydrogenase/genetics/*metabolism, Biocatalysis, Escherichia coli/*metabolism, Genetic Vectors/genetics/metabolism, NADH, NADPH Oxidoreductases/genetics/*metabolism, Optical Rotation, Quinuclidines/chemistry/*metabolism, Stereoisomerism, Time FactorsMetabolism
Enzymology23263947Gene cloning and characterization of two NADH-dependent 3-quinuclidinone reductases from Microbacterium luteolum JCM 9174.Isotani K, Kurokawa J, Suzuki F, Nomoto S, Negishi T, Matsuda M, Itoh NAppl Environ Microbiol10.1128/AEM.03099-122012Actinomycetales/*enzymology/genetics, Amino Acid Sequence, Cloning, Molecular, Coenzymes/*metabolism, DNA, Bacterial/chemistry/genetics, Escherichia coli/genetics, Molecular Sequence Data, NAD/*metabolism, Oxidoreductases/*genetics/*metabolism, Quinuclidines/*metabolism, Recombinant Fusion Proteins/genetics/isolation & purification, Sequence Alignment, Sequence Analysis, DNA, Substrate SpecificityMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8567Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20143)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20143
18424Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20143.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40319Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13732
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
76825Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8204.1StrainInfo: A central database for resolving microbial strain identifiers
120693Curators of the CIPCollection of Institut Pasteur (CIP 102086)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102086