Strain identifier

BacDive ID: 7342

Type strain: Yes

Species: Leifsonia poae

Strain history: CIP <- 2000, L. Evtushenko, VKM, Moscow, Russia: strain Ac-1401

NCBI tax ID(s): 110933 (species)

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General

@ref: 5755

BacDive-ID: 7342

DSM-Number: 15202

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Leifsonia poae CCUG 47140 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from nematode gall on Poa annua root.

NCBI tax id

  • NCBI tax id: 110933
  • Matching level: species

strain history

@refhistory
5755<- CIP <- VKM
67770CIP 106654 <-- L. I. Evtushenko VKM Ac-1401.
121916CIP <- 2000, L. Evtushenko, VKM, Moscow, Russia: strain Ac-1401

doi: 10.13145/bacdive7342.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Leifsonia
  • species: Leifsonia poae
  • full scientific name: Leifsonia poae Evtushenko et al. 2000

@ref: 5755

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Leifsonia

species: Leifsonia poae

full scientific name: Leifsonia poae Evtushenko et al. 2000

type strain: yes

Morphology

cell morphology

  • @ref: 121916
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 121916

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5755TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
35974MEDIUM 111 - for ClavibacteryesDistilled water make up to (1000.000 ml);Sodium chloride (5.000 g);Agar(15.000 g);Glucose (5.000 g);Yeast extract (5.000 g);Trypticase peptone (10.000 g)
121916CIP Medium 111yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=111

culture temp

@refgrowthtypetemperaturerange
5755positivegrowth28mesophilic
35974positivegrowth20psychrophilic
57533positivegrowth30mesophilic
67770positivegrowth28mesophilic
121916positivegrowth15-30
121916nogrowth10psychrophilic
121916nogrowth37mesophilic
121916nogrowth41thermophilic
121916nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57533aerobe
121916obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
121916NaClpositivegrowth0-2 %
121916NaClnogrowth4 %
121916NaClnogrowth6 %
121916NaClnogrowth8 %
121916NaClnogrowth10 %

murein

  • @ref: 5755
  • murein short key: B07
  • type: B2gamma {Gly} [L-Dab] D-Glu-D-Dab

observation

  • @ref: 67770
  • observation: quinones: MK-11, MK-10, MK-12

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12191616947citrate-carbon source
1219164853esculin+hydrolysis
121916606565hippurate-hydrolysis
12191617632nitrate-reduction
12191616301nitrite-reduction
12191617632nitrate-respiration

antibiotic resistance

  • @ref: 121916
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 121916
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12191615688acetoin-
12191617234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121916oxidase+
121916beta-galactosidase+3.2.1.23
121916alcohol dehydrogenase-1.1.1.1
121916gelatinase-
121916amylase-
121916DNase+
121916caseinase-3.4.21.50
121916catalase+1.11.1.6
121916tween esterase-
121916gamma-glutamyltransferase-2.3.2.2
121916lecithinase-
121916lipase-
121916lysine decarboxylase-4.1.1.18
121916ornithine decarboxylase-4.1.1.17
121916phenylalanine ammonia-lyase-4.3.1.24
121916protease-
121916tryptophan deaminase-
121916urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121916--++-++---++++-+++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121916+++++--+-++-+--+++++++++-+-----+-+++------------------+----++--------------+----------+---+--------

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrysampling dategeographic locationcontinentisolation date
5755nematode gall on Poa annua rootPoa annuaRussiaRUS
57533Poa annua root gallRussiaRUS1993Moscow regionEurope
67770Poa annua root galls induced by the grass root gall nematode Subanguina radicicolaSubanguina radicicolaRussiaRUSMoscow Region
121916Plant, Poa annua root gallRussian FederationRUSMoscow regionEurope1993

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Nematoda
#Host#Plants#Herbaceous plants (Grass,Crops)
#Infection#Plant infections#Gall
#Host Body-Site#Plant#Root (Rhizome)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1148.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_671;97_777;98_901;99_1148&stattab=map
  • Last taxonomy: Leifsonia
  • 16S sequence: AM410682
  • Sequence Identity:
  • Total samples: 3840
  • soil counts: 2218
  • aquatic counts: 554
  • animal counts: 672
  • plant counts: 396

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
57551Risk group (German classification)
1219161Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Leifsonia poae partial 16S rRNA gene, type strain DSM 15202AM4106821488ena110933
20218Leifsonia poae strain VKM Ac-1401 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ2326132111ena110933
5755Leifsonia poae 16S ribosomal RNA, partial sequenceAF1163421488ena110933

External links

@ref: 5755

culture collection no.: CCUG 47140, DSM 15202, CIP 106654, VKM Ac-1401, JCM 11952, IAM 15136, KCTC 19442, NBRC 103069

straininfo link

  • @ref: 76792
  • straininfo: 72045

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10826825Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of 'Corynebacterium aquaticum' Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov.Evtushenko LI, Dorofeeva LV, Subbotin SA, Cole JR, Tiedje JMInt J Syst Evol Microbiol10.1099/00207713-50-1-3712000Actinomycetales/*classification/cytology/isolation & purification/physiology, Animals, Cell Wall/chemistry, Culture Media, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, *Nematoda, Nucleic Acid Hybridization, Phenotype, Plant Tumors/*microbiology/parasitology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNACultivation
Phylogeny17267987Leifsonia ginsengi sp. nov., isolated from ginseng root.Qiu F, Huang Y, Sun L, Zhang X, Liu Z, Song WInt J Syst Evol Microbiol10.1099/ijs.0.64487-02007Actinomycetales/*classification/cytology/*isolation & purification/physiology, Aminobutyrates/analysis, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, rRNA, Molecular Sequence Data, Movement, Panax/*microbiology, Peptidoglycan/chemistry, Phylogeny, Plant Roots/*microbiology, Quinones/analysis/chemistry, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidGenetics
Phylogeny19126716Leifsonia kribbensis sp. nov., isolated from soil.Dastager SG, Lee JC, Ju YJ, Park DJ, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.001925-02009Actinomycetales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Korea, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, *Soil Microbiology, Species SpecificityGenetics
Phylogeny23728372Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil.Kim SJ, Moon JY, Hamada M, Tamura T, Weon HY, Suzuki KI, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.049817-02013Actinomycetales/*classification/genetics/isolation & purification, Aminobutyrates/chemistry, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Environment, Controlled, Fatty Acids/chemistry, Molecular Sequence Data, Peptidoglycan/chemistry, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5755Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15202)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15202
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35974Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18808
57533Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47140)https://www.ccug.se/strain?id=47140
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76792Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID72045.1StrainInfo: A central database for resolving microbial strain identifiers
121916Curators of the CIPCollection of Institut Pasteur (CIP 106654)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106654