Strain identifier
BacDive ID: 7240
Type strain:
Species: Methylophilus glucosoxydans
Strain Designation: B
Variant: Isotype of BacDive ID 131041
Strain history: <- Acad. L. Kalakouzky and M. Vainstein, IBPhM USSR Acad Sci.
NCBI tax ID(s): 752553 (species)
General
@ref: 2155
BacDive-ID: 7240
DSM-Number: 5898
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Methylophilus glucosoxydans B is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of rice .
NCBI tax id
- NCBI tax id: 752553
- Matching level: species
strain history
- @ref: 2155
- history: <- Acad. L. Kalakouzky and M. Vainstein, IBPhM USSR Acad Sci.
doi: 10.13145/bacdive7240.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Nitrosomonadales
- family: Methylophilaceae
- genus: Methylophilus
- species: Methylophilus glucosoxydans
- full scientific name: Methylophilus glucosoxydans Doronina et al. 2012
@ref: 2155
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Methylophilales
family: Methylophilaceae
genus: Methylophilus
species: Methylophilus glucosoxydans
full scientific name: Methylophilus glucosoxydans Doronina et al. 2012
strain designation: B
variant: Isotype of BacDive ID 131041
type strain: yes
Morphology
cell morphology
- @ref: 29893
- gram stain: negative
- cell length: 1.0-2.5 µm
- cell width: 0.3-0.5 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 29893
- production: yes
Culture and growth conditions
culture medium
- @ref: 2155
- name: 2% MH MEDIUM (DSMZ Medium 620)
- growth: yes
- link: https://mediadive.dsmz.de/medium/620
- composition: Name: 2% MH MEDIUM (DSMZ Medium 620) Composition: Methanol 20.0 g/l (NH4)2SO4 2.0 g/l KH2PO4 2.0 g/l Glucose 1.5 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.025 g/l FeSO4 x 7 H2O 0.002 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2155 | positive | growth | 28 | mesophilic |
29893 | positive | growth | 10-34 | |
29893 | positive | optimum | 28-30 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
29893 | positive | growth | 6.5-8.0 |
29893 | positive | optimum | 7.0-7.6 |
Physiology and metabolism
oxygen tolerance
- @ref: 29893
- oxygen tolerance: aerobe
spore formation
- @ref: 29893
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
29893 | NaCl | positive | growth | 0.05-0.5 % |
29893 | NaCl | positive | optimum | 0.05 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29893 | 17234 | glucose | + | carbon source |
29893 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 29893
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
- @ref: 29893
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
29893 | cytochrome oxidase | + | 1.9.3.1 |
29893 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 2155
- sample type: rhizosphere of rice (Oryza sativa L.)
- host species: Oryza sativa
- country: Vietnam
- origin.country: VNM
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
Safety information
risk assessment
- @ref: 2155
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 2155
- description: Methylophilus glucosoxydans strain B 16S ribosomal RNA gene, partial sequence
- accession: HM001269
- length: 1387
- database: ena
- NCBI tax ID: 752553
GC content
@ref | GC-content | method |
---|---|---|
2155 | 52.5 | thermal denaturation, midpoint method (Tm) |
29893 | 52.5 |
External links
@ref: 2155
culture collection no.: DSM 5898, CCUG 59685, VKM B-1607
straininfo link
- @ref: 76692
- straininfo: 48023
literature
- topic: Phylogeny
- Pubmed-ID: 21378135
- title: Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere.
- authors: Doronina NV, Gogleva AA, Trotsenko YA
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.024620-0
- year: 2011
- mesh: Aerobiosis, Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Indoleacetic Acids/metabolism, Locomotion, Methanol/*metabolism, Methylophilus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nitrogen/metabolism, Nucleic Acid Hybridization, Oryza/*microbiology, Phospholipids/analysis, Phylogeny, Plant Roots/*microbiology, Quaternary Ammonium Compounds/metabolism, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Ubiquinone/analysis
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
2155 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5898) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5898 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29893 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26266 | 28776041 | |
76692 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48023.1 | StrainInfo: A central database for resolving microbial strain identifiers |