Strain identifier

BacDive ID: 7240

Type strain: Yes

Species: Methylophilus glucosoxydans

Strain Designation: B

Variant: Isotype of BacDive ID 131041

Strain history: <- Acad. L. Kalakouzky and M. Vainstein, IBPhM USSR Acad Sci.

NCBI tax ID(s): 752553 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2155

BacDive-ID: 7240

DSM-Number: 5898

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Methylophilus glucosoxydans B is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of rice .

NCBI tax id

  • NCBI tax id: 752553
  • Matching level: species

strain history

  • @ref: 2155
  • history: <- Acad. L. Kalakouzky and M. Vainstein, IBPhM USSR Acad Sci.

doi: 10.13145/bacdive7240.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Nitrosomonadales
  • family: Methylophilaceae
  • genus: Methylophilus
  • species: Methylophilus glucosoxydans
  • full scientific name: Methylophilus glucosoxydans Doronina et al. 2012

@ref: 2155

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Methylophilales

family: Methylophilaceae

genus: Methylophilus

species: Methylophilus glucosoxydans

full scientific name: Methylophilus glucosoxydans Doronina et al. 2012

strain designation: B

variant: Isotype of BacDive ID 131041

type strain: yes

Morphology

cell morphology

  • @ref: 29893
  • gram stain: negative
  • cell length: 1.0-2.5 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 29893
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 2155
  • name: 2% MH MEDIUM (DSMZ Medium 620)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/620
  • composition: Name: 2% MH MEDIUM (DSMZ Medium 620) Composition: Methanol 20.0 g/l (NH4)2SO4 2.0 g/l KH2PO4 2.0 g/l Glucose 1.5 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.025 g/l FeSO4 x 7 H2O 0.002 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
2155positivegrowth28mesophilic
29893positivegrowth10-34
29893positiveoptimum28-30mesophilic

culture pH

@refabilitytypepH
29893positivegrowth6.5-8.0
29893positiveoptimum7.0-7.6

Physiology and metabolism

oxygen tolerance

  • @ref: 29893
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29893
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29893NaClpositivegrowth0.05-0.5 %
29893NaClpositiveoptimum0.05 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2989317234glucose+carbon source
2989317632nitrate+reduction

metabolite production

  • @ref: 29893
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 29893
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
29893cytochrome oxidase+1.9.3.1
29893urease+3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 2155
  • sample type: rhizosphere of rice (Oryza sativa L.)
  • host species: Oryza sativa
  • country: Vietnam
  • origin.country: VNM
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 2155
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 2155
  • description: Methylophilus glucosoxydans strain B 16S ribosomal RNA gene, partial sequence
  • accession: HM001269
  • length: 1387
  • database: ena
  • NCBI tax ID: 752553

GC content

@refGC-contentmethod
215552.5thermal denaturation, midpoint method (Tm)
2989352.5

External links

@ref: 2155

culture collection no.: DSM 5898, CCUG 59685, VKM B-1607

straininfo link

  • @ref: 76692
  • straininfo: 48023

literature

  • topic: Phylogeny
  • Pubmed-ID: 21378135
  • title: Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere.
  • authors: Doronina NV, Gogleva AA, Trotsenko YA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.024620-0
  • year: 2011
  • mesh: Aerobiosis, Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Indoleacetic Acids/metabolism, Locomotion, Methanol/*metabolism, Methylophilus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nitrogen/metabolism, Nucleic Acid Hybridization, Oryza/*microbiology, Phospholipids/analysis, Phylogeny, Plant Roots/*microbiology, Quaternary Ammonium Compounds/metabolism, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Ubiquinone/analysis
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
2155Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5898)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5898
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29893Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2626628776041
76692Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48023.1StrainInfo: A central database for resolving microbial strain identifiers