Strain identifier

BacDive ID: 7221

Type strain: Yes

Species: Albibacter helveticus

Strain Designation: DM9

Strain history: CIP <- 2000, Y.A. Trotsenko, Inst. Bioch. Physiol. Microorg., RAS, Pushchino, Russia: strain DM9 <- T. Lieisinger <- R. Galli

NCBI tax ID(s): 119025 (species)

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General

@ref: 16520

BacDive-ID: 7221

DSM-Number: 22800

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Albibacter helveticus DM9 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from groundwater from contaminated environment.

NCBI tax id

  • NCBI tax id: 119025
  • Matching level: species

strain history

@refhistory
16520<- CIP <- Y. A. Trotsenko, Inst. Marine Biol. FEB RAS, Vladivostok; DM9 <- T. Leisinger <- R. Galli
119864CIP <- 2000, Y.A. Trotsenko, Inst. Bioch. Physiol. Microorg., RAS, Pushchino, Russia: strain DM9 <- T. Lieisinger <- R. Galli

doi: 10.13145/bacdive7221.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylocystaceae
  • genus: Albibacter
  • species: Albibacter helveticus
  • full scientific name: Albibacter helveticus (Doronina et al. 2000) Yang et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Methylopila helvetica

@ref: 16520

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylocystaceae

genus: Albibacter

species: Albibacter helveticus

full scientific name: Albibacter helveticus (Doronina et al. 2000) Yang et al. 2016

strain designation: DM9

type strain: yes

Morphology

cell morphology

  • @ref: 119864
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 119864
  • production: no
  • name: Pyocyanin

multimedia

  • @ref: 16520
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_22800.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16520NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Agar 15.0 g/l Methanol 5.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
16520MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
119864CIP Medium 54yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=54

culture temp

@refgrowthtypetemperaturerange
16520positivegrowth28mesophilic
119864positivegrowth25-30mesophilic
119864nogrowth5psychrophilic
119864nogrowth10psychrophilic
119864nogrowth37mesophilic
119864nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
119864NaClpositivegrowth0-2 %
119864NaClnogrowth4 %
119864NaClnogrowth6 %
119864NaClnogrowth8 %
119864NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1198644853esculin-hydrolysis
11986417632nitrate+reduction
11986416301nitrite-reduction
11986417632nitrate-respiration

antibiotic resistance

  • @ref: 119864
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119864
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
119864oxidase+
119864beta-galactosidase+3.2.1.23
119864alcohol dehydrogenase-1.1.1.1
119864gelatinase-
119864amylase-
119864DNase-
119864caseinase-3.4.21.50
119864catalase+1.11.1.6
119864tween esterase-
119864lecithinase-
119864lipase-
119864lysine decarboxylase-4.1.1.18
119864ornithine decarboxylase-4.1.1.17
119864tryptophan deaminase-
119864urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119864--------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119864------------+------------------------------------+----------+------------------++---------+--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
16520groundwater from contaminated environmentSwitzerlandCHEEurope
119864Environment, GroundwaterSwitzerlandCHEEurope1986

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Aquatic#Groundwater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
165201Risk group (German classification)
1198641Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16520
  • description: Methylopila helvetica strain DM9 (T) 16S ribosomal RNA, partial sequence
  • accession: AF227126
  • length: 1377
  • database: ena
  • NCBI tax ID: 119025

External links

@ref: 16520

culture collection no.: DSM 22800, CIP 106788, VKM B-2189

straininfo link

  • @ref: 76673
  • straininfo: 69240

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10930073Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov.--novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane.Doronina NV, Trotsenko YA, Tourova TP, Kuznetsov BB, Leisinger TSyst Appl Microbiol10.1016/S0723-2020(00)80007-72000Bacterial Typing Techniques, Biodegradation, Environmental, DNA, Ribosomal/genetics, Gram-Negative Aerobic Bacteria/classification/*metabolism/ultrastructure, Methylene Chloride/*metabolism, Methylobacterium/classification/*metabolism/ultrastructure, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny27046027Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter.Yang LQ, Liu L, Salam N, Xiao M, Kim CJ, Hozzein WN, Park DJ, Li WJ, Zhang HWInt J Syst Evol Microbiol10.1099/ijsem.0.0010622016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylocystaceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/analogs & derivatives/analysisTranscriptome

Reference

@idauthorscataloguedoi/urltitle
16520Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22800)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22800
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68382Automatically annotated from API zym
76673Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69240.1StrainInfo: A central database for resolving microbial strain identifiers
119864Curators of the CIPCollection of Institut Pasteur (CIP 106788)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106788