Strain identifier
BacDive ID: 7217
Type strain: 
Species: Methylocystis rosea
Strain Designation: SV97
Strain history: <- M. M. Svenning; SV97 <- A. G. Hestnes
NCBI tax ID(s): 173366 (species)
version 8.1 (current version)
General
@ref: 6861
BacDive-ID: 7217
DSM-Number: 17261
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped
description: Methylocystis rosea SV97 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from a high Artic Wetland soil.
NCBI tax id
- NCBI tax id: 173366
- Matching level: species
strain history
- @ref: 6861
- history: <- M. M. Svenning; SV97 <- A. G. Hestnes
doi: 10.13145/bacdive7217.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylocystaceae
- genus: Methylocystis
- species: Methylocystis rosea
- full scientific name: Methylocystis rosea Wartiainen et al. 2006
@ref: 6861
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylocystaceae
genus: Methylocystis
species: Methylocystis rosea
full scientific name: Methylocystis rosea Wartiainen et al. 2006
strain designation: SV97
type strain: yes
Morphology
cell morphology
- @ref: 31620
- gram stain: negative
- cell length: 1.1-2.5 µm
- cell width: 0.8-1.1 µm
- cell shape: rod-shaped
- motility: no
pigmentation
- @ref: 31620
- production: yes
Culture and growth conditions
culture medium
- @ref: 6861
- name: METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM (DSMZ Medium 921)
- growth: yes
- link: https://mediadive.dsmz.de/medium/921
- composition: Name: METHYLOSARCINA QUISQUILLARUM AND M. FIBRATA MEDIUM (DSMZ Medium 921) Composition: Agar 15.0 g/l MgSO4 x 6 H2O 1.0 g/l KNO3 1.0 g/l Na2HPO4 x 12 H2O 0.716 g/l KH2PO4 0.26 g/l CaCl2 x 2 H2O 0.2 g/l CuSO4 x 5 H2O 0.001 g/l FeSO4 x 7 H2O 0.0005 g/l ZnSO4 x 7 H2O 0.0004 g/l Fe(III)-EDTA 0.00038 g/l Na2MoO4 x 2 H2O 0.00026 g/l Na2-EDTA 0.00025 g/l CoCl2 x 6 H2O 5e-05 g/l MnCl2 x 4 H2O 2e-05 g/l H3BO3 1.5e-05 g/l NiCl2 x 6 H2O 1e-05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6861 | positive | growth | 28 | mesophilic |
| 31620 | positive | growth | 27-37 | mesophilic |
| 31620 | positive | optimum | 27 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31620 | positive | growth | 5.5-9 | alkaliphile |
| 31620 | positive | optimum | 5.5-9 |
Physiology and metabolism
spore formation
- @ref: 31620
- spore formation: no
observation
- @ref: 31620
- observation: aggregates in chains
enzymes
- @ref: 31620
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
- @ref: 6861
- sample type: a high Artic Wetland soil
- geographic location: Ny-Alesund, Svalbard Islands
- country: Norway
- origin.country: NOR
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Environmental | #Terrestrial | #Wetland (Swamp) |
| #Condition | #Psychrophilic (<10°C) | |
| #Climate | #Cold | #Polar |
taxonmaps
- @ref: 69479
- File name: preview.99_302.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_208;97_227;98_256;99_302&stattab=map
- Last taxonomy: Methylocystis
- 16S sequence: AJ414656
- Sequence Identity:
- Total samples: 1645
- soil counts: 493
- aquatic counts: 940
- animal counts: 169
- plant counts: 43
Safety information
risk assessment
- @ref: 6861
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6861
- description: Methylocystis rosea 16S ribosomal RNA, type strain SV97T
- accession: AJ414656
- length: 1438
- database: ena
- NCBI tax ID: 1132444
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methylocystis rosea SV97 | GCA_000372845 | scaffold | ncbi | 1132444 |
| 66792 | Methylocystis rosea SV97 | 1132444.3 | wgs | patric | 1132444 |
GC content
- @ref: 6861
- GC-content: 62
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 88.569 | yes |
| flagellated | no | 97.008 | yes |
| gram-positive | no | 98.055 | yes |
| anaerobic | no | 97.21 | no |
| aerobic | yes | 79.595 | no |
| halophile | no | 93.791 | no |
| spore-forming | no | 93.877 | yes |
| thermophile | no | 97.88 | yes |
| glucose-util | yes | 73.646 | no |
| glucose-ferment | no | 93.899 | no |
External links
@ref: 6861
culture collection no.: DSM 17261, ATCC BAA 1196
straininfo link
- @ref: 76669
- straininfo: 290993
literature
- topic: Phylogeny
- Pubmed-ID: 16514024
- title: Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78 degrees N).
- authors: Wartiainen I, Hestnes AG, McDonald IR, Svenning MM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63912-0
- year: 2006
- mesh: Arctic Regions, Base Composition, DNA, Ribosomal/chemistry, Methylocystaceae/*classification/genetics/isolation & purification/physiology, Norway, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
- topic2: Transcriptome
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6861 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17261) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17261 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31620 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27906 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76669 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID290993.1 | StrainInfo: A central database for resolving microbial strain identifiers |