Strain identifier

BacDive ID: 7215

Type strain: Yes

Species: Albibacter methylovorans

Strain Designation: DM 10

Strain history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia

NCBI tax ID(s): 128802 (species)

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General

@ref: 16420

BacDive-ID: 7215

DSM-Number: 22840

keywords: 16S sequence, Bacteria, mesophilic

description: Albibacter methylovorans DM 10 is a mesophilic bacterium that was isolated from ground water contaminated with dichloromethane.

NCBI tax id

  • NCBI tax id: 128802
  • Matching level: species

strain history

  • @ref: 16420
  • history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia

doi: 10.13145/bacdive7215.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylocystaceae
  • genus: Albibacter
  • species: Albibacter methylovorans
  • full scientific name: Albibacter methylovorans Doronina et al. 2001

@ref: 16420

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylocystaceae

genus: Albibacter

species: Albibacter methylovorans

full scientific name: Albibacter methylovorans Doronina et al. 2001

strain designation: DM 10

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16420MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462)yeshttps://mediadive.dsmz.de/medium/462Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Peptone 5.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l p-Aminobenzoate 2.5e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water
16420NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 16420
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 16420
  • sample type: ground water contaminated with dichloromethane
  • country: Switzerland
  • origin.country: CHE
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Aquatic#Groundwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_114845.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_1101;97_1289;98_5709;99_114845&stattab=map
  • Last taxonomy: Albibacter methylovorans
  • 16S sequence: FR733694
  • Sequence Identity:
  • Total samples: 21
  • soil counts: 4
  • aquatic counts: 6
  • animal counts: 10
  • plant counts: 1

Safety information

risk assessment

  • @ref: 16420
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Albibacter methylovorans partial 16S rRNA gene, type strain DSM22840TFR7336941476ena128802
16420Albobacter methylovorans 16S ribosomal RNA gene, partial sequenceAF2732131409ena128802

GC content

  • @ref: 16420
  • GC-content: 66.7
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 16420

culture collection no.: DSM 22840, DSM 13819, VKM B-2236

straininfo link

  • @ref: 76667
  • straininfo: 49528

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411673Albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane.Doronina NV, Trotsenko YA, Tourova TP, Kuznetsov BB, Leisinger TInt J Syst Evol Microbiol10.1099/00207713-51-3-10512001DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Gram-Negative Bacteria/*classification/genetics/metabolism/ultrastructure, Methylene Chloride/*metabolism, Microscopy, Electron, Molecular Sequence Data, Nucleic Acid Hybridization, Pentose Phosphate Pathway, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny27046027Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter.Yang LQ, Liu L, Salam N, Xiao M, Kim CJ, Hozzein WN, Park DJ, Li WJ, Zhang HWInt J Syst Evol Microbiol10.1099/ijsem.0.0010622016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylocystaceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/analogs & derivatives/analysisTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16420Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22840)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22840
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76667Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49528.1StrainInfo: A central database for resolving microbial strain identifiers