Strain identifier
BacDive ID: 72
Type strain:
Species: Roseomonas aquatica
Strain Designation: TR53
Strain history: CIP <- 2006, CECT <- A. Ventosa: strain TR53
NCBI tax ID(s): 373043 (species)
General
@ref: 8017
BacDive-ID: 72
DSM-Number: 19438
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, colony-forming
description: Roseomonas aquatica TR53 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms irregular colonies and was isolated from drinking water distribution system of Seville.
NCBI tax id
- NCBI tax id: 373043
- Matching level: species
strain history
@ref | history |
---|---|
8017 | <- JCM/RIKEN <- A. Ventosa, Univ. Seville, Dept. Microbiol. Parasitol., Spain; TR53 |
67770 | A. Ventosa TR53. |
116433 | CIP <- 2006, CECT <- A. Ventosa: strain TR53 |
doi: 10.13145/bacdive72.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Roseomonas
- species: Roseomonas aquatica
- full scientific name: Roseomonas aquatica Gallego et al. 2006
synonyms
- @ref: 20215
- synonym: Paeniroseomonas aquatica
@ref: 8017
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Roseomonas
species: Roseomonas aquatica
full scientific name: Roseomonas aquatica Gallego et al. 2006 emend. Sánchez-Porro et al. 2009
strain designation: TR53
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | motility | cell shape |
---|---|---|---|---|---|
22959 | negative | 1.2-2.0 µm | 1.0 µm | no | |
116433 | negative | no | oval-shaped |
colony morphology
@ref | colony size | colony color | colony shape | incubation period | medium used |
---|---|---|---|---|---|
22959 | 0.5-1.0 mm | pale-pink-pigmented | irregular | 4 days | R2A Agar |
22959 | <0.5 mm | nutrient agar medium |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8017 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
22959 | nutrient agar medium | yes | ||
22959 | Reasoner's 2A agar (R2A) | yes | ||
37542 | MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati | yes | Distilled water make up to (1000.000 ml);R2A agar (18.200 g) | |
116433 | CIP Medium 566 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8017 | positive | growth | 28 | mesophilic |
22959 | positive | growth | 15.0-35.0 | |
22959 | positive | optimum | 25.0-28.0 | mesophilic |
37542 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
22959 | positive | growth | 5.0-9.0 | alkaliphile |
22959 | positive | optimum | 7.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 22959
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 22959
- spore formation: no
halophily
- @ref: 22959
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: >2.0 %
observation
- @ref: 67770
- observation: quinones: Q-10
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22959 | 15824 | D-fructose | - | builds acid from |
22959 | 12936 | D-galactose | - | builds acid from |
22959 | 17634 | D-glucose | - | builds acid from |
22959 | 17306 | maltose | - | builds acid from |
22959 | 16899 | D-mannitol | - | builds acid from |
22959 | 16024 | D-mannose | - | builds acid from |
22959 | 16551 | D-trehalose | - | builds acid from |
22959 | 65327 | D-xylose | - | builds acid from |
22959 | 17754 | glycerol | - | builds acid from |
22959 | 17716 | lactose | - | builds acid from |
22959 | 53258 | citric acid | - | carbon source |
22959 | 16808 | 2-dehydro-D-gluconate | - | carbon source |
22959 | 17426 | 5-dehydro-D-gluconate | - | carbon source |
22959 | 17128 | adipate | - | carbon source |
22959 | 27613 | amygdalin | - | carbon source |
22959 | 18305 | arbutin | - | carbon source |
22959 | 27689 | decanoate | - | carbon source |
22959 | 15963 | ribitol | - | carbon source |
22959 | 17108 | D-arabinose | - | carbon source |
22959 | 18333 | D-arabitol | - | carbon source |
22959 | 17057 | cellobiose | - | carbon source |
22959 | 15824 | D-fructose | - | carbon source |
22959 | 28847 | D-fucose | - | carbon source |
22959 | 12936 | D-galactose | - | carbon source |
22959 | 17634 | D-glucose | - | carbon source |
22959 | 17716 | lactose | - | carbon source |
22959 | 62318 | D-lyxose | - | carbon source |
22959 | 17306 | maltose | - | carbon source |
22959 | 16899 | D-mannitol | - | carbon source |
22959 | 16024 | D-mannose | - | carbon source |
22959 | 6731 | melezitose | - | carbon source |
22959 | 28053 | melibiose | - | carbon source |
22959 | 16634 | raffinose | - | carbon source |
22959 | 16988 | D-ribose | - | carbon source |
22959 | 17924 | D-sorbitol | - | carbon source |
22959 | 16443 | D-tagatose | - | carbon source |
22959 | 16551 | D-trehalose | - | carbon source |
22959 | 32528 | turanose | - | carbon source |
22959 | 65327 | D-xylose | - | carbon source |
22959 | 16813 | galactitol | - | carbon source |
22959 | 17113 | erythritol | - | carbon source |
22959 | 4853 | esculin | - | carbon source |
22959 | 28066 | gentiobiose | - | carbon source |
22959 | 17754 | glycerol | - | carbon source |
22959 | 28087 | glycogen | - | carbon source |
22959 | 17268 | myo-inositol | - | carbon source |
22959 | 15443 | inulin | - | carbon source |
22959 | 30849 | L-arabinose | - | carbon source |
22959 | 18287 | L-fucose | - | carbon source |
22959 | 18403 | L-arabitol | - | carbon source |
22959 | 62345 | L-rhamnose | - | carbon source |
22959 | 17266 | L-sorbose | - | carbon source |
22959 | 65328 | L-xylose | - | carbon source |
22959 | 25115 | malate | - | carbon source |
22959 | 320061 | methyl alpha-D-glucopyranoside | - | carbon source |
22959 | 43943 | methyl alpha-D-mannoside | - | carbon source |
22959 | 74863 | methyl beta-D-xylopyranoside | - | carbon source |
22959 | 506227 | N-acetylglucosamine | - | carbon source |
22959 | 18401 | phenylacetate | - | carbon source |
22959 | 32032 | potassium gluconate | - | carbon source |
22959 | 17814 | salicin | - | carbon source |
22959 | 53258 | sodium citrate | - | carbon source |
22959 | 28017 | starch | - | carbon source |
22959 | 17992 | sucrose | - | carbon source |
22959 | 17151 | xylitol | - | carbon source |
22959 | 27897 | tryptophan | - | energy source |
22959 | 17234 | glucose | - | fermentation |
22959 | casein | - | hydrolysis | |
22959 | 16991 | dna | - | hydrolysis |
22959 | 5291 | gelatin | - | hydrolysis |
22959 | 28017 | starch | - | hydrolysis |
22959 | 53426 | tween 80 | - | hydrolysis |
22959 | 17632 | nitrate | + | reduction |
116433 | 17632 | nitrate | + | reduction |
116433 | 16301 | nitrite | - | reduction |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. |
---|---|---|---|---|---|
22959 | 28669 | bacitracin | yes | yes | 10 Unit |
22959 | 17334 | penicillin | yes | yes | 10 Unit |
22959 | 7507 | neomycin | yes | yes | 10 µg (disc) |
22959 | 17076 | streptomycin | yes | yes | 10 µg (disc) |
22959 | 48923 | erythromycin | yes | yes | 15 µg (disc) |
22959 | 3542 | cephalothin | yes | yes | 30 µg (disc) |
22959 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) |
22959 | 6104 | kanamycin | yes | yes | 30 µg (disc) |
22959 | 100147 | nalidixic acid | yes | yes | 30 µg (disc) |
22959 | 28368 | novobiocin | yes | yes | 30 µg (disc) |
22959 | 28077 | rifampicin | yes | yes | 30 µg (disc) |
22959 | 27902 | tetracycline | yes | yes | 30 µg (disc) |
22959 | 28001 | vancomycin | yes | yes | 30 µg (disc) |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
22959 | 35581 | indole | no |
22959 | 16136 | hydrogen sulfide | no |
22959 | 15688 | acetoin | no |
116433 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test | indole test | citrate test |
---|---|---|---|---|---|---|
22959 | 15688 | acetoin | - | |||
22959 | 17234 | glucose | - | |||
22959 | 35581 | indole | - | |||
22959 | 16947 | citrate | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
22959 | acid phosphatase | + | 3.1.3.2 |
22959 | alkaline phosphatase | + | 3.1.3.1 |
22959 | alpha-chymotrypsin | - | 3.4.21.1 |
22959 | alpha-fucosidase | - | 3.2.1.51 |
22959 | alpha-galactosidase | - | 3.2.1.22 |
22959 | alpha-glucosidase | - | 3.2.1.20 |
22959 | alpha-mannosidase | - | 3.2.1.24 |
22959 | arginine dihydrolase | - | 3.5.3.6 |
22959 | beta-galactosidase | - | 3.2.1.23 |
22959 | beta-glucosidase | - | 3.2.1.21 |
22959 | beta-glucuronidase | - | 3.2.1.31 |
22959 | catalase | + | 1.11.1.6 |
22959 | cystine arylamidase | - | 3.4.11.3 |
22959 | cytochrome oxidase | - | 1.9.3.1 |
22959 | esterase (C 4) | + | |
22959 | esterase lipase (C 8) | + | |
22959 | leucine arylamidase | + | 3.4.11.1 |
22959 | lipase (C 14) | - | |
22959 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
22959 | naphthol-AS-BI-phosphohydrolase | + | |
22959 | trypsin | - | 3.4.21.4 |
22959 | tryptophan deaminase | - | 4.1.99.1 |
22959 | urease | + | 3.5.1.5 |
22959 | valine arylamidase | - | |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
116433 | oxidase | - | |
116433 | catalase | + | 1.11.1.6 |
116433 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116433 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | latitude | longitude | enrichment culture | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|
8017 | drinking water distribution system of Seville | Spain | ESP | Europe | ||||||
22959 | potable water | Spain | ESP | Europe | Seville | 37.3925 | -5.9925 | plate count agar and R2A | 7 days | 28.0 |
67770 | Drinking water distribution system of Seville | Spain | ESP | Europe | ||||||
116433 | Environment, Drinking water | Spain | ESP | Europe | Sevilla |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Freshwater
taxonmaps
- @ref: 69479
- File name: preview.99_9162.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_266;96_4375;97_5327;98_6734;99_9162&stattab=map
- Last taxonomy: Roseomonas aquatica subclade
- 16S sequence: AM231587
- Sequence Identity:
- Total samples: 3865
- soil counts: 1130
- aquatic counts: 761
- animal counts: 1125
- plant counts: 849
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
8017 | 1 | Risk group (German classification) |
116433 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 8017
- description: Roseomonas aquatica partial 16S rRNA gene, type strain TR53T
- accession: AM231587
- length: 1387
- database: ena
- NCBI tax ID: 373043
GC content
- @ref: 8017
- GC-content: 68.6
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 8017
culture collection no.: DSM 19438, CECT 7131, JCM 13556, CCM 7358, NCIMB 14503, CIP 109393
straininfo link
- @ref: 69756
- straininfo: 297309
literature
- topic: Phylogeny
- Pubmed-ID: 17012549
- title: Roseomonas aquatica sp. nov., isolated from drinking water.
- authors: Gallego V, Sanchez-Porro C, Garcia MT, Ventosa A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.64379-0
- year: 2006
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Methylobacteriaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water Supply
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8017 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19438) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19438 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
22959 | Virginia Gallego,Cristina Sánchez-Porro,Maria Teresa García,Antonio Ventosa | 10.1099/ijs.0.64379-0 | Roseomonas aquatica sp. nov., isolated from drinking water | IJSEM 56: 2291-2295 2006 | 17012549 | |
37542 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7130 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69756 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297309.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116433 | Curators of the CIP | Collection of Institut Pasteur (CIP 109393) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109393 |