Strain identifier
BacDive ID: 7197
Type strain:
Species: Methylobacterium cerastii
Strain Designation: C15, C20oh1-15
Strain history: <- P. Kämpfer, Justus-Liebig-Universität; C20oh1-15 <- S.A. Wellner
NCBI tax ID(s): 932741 (species)
General
@ref: 17341
BacDive-ID: 7197
DSM-Number: 23679
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Methylobacterium cerastii C15 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from leaf surface of Cerastium holosteoides.
NCBI tax id
- NCBI tax id: 932741
- Matching level: species
strain history
- @ref: 17341
- history: <- P. Kämpfer, Justus-Liebig-Universität; C20oh1-15 <- S.A. Wellner
doi: 10.13145/bacdive7197.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium cerastii
- full scientific name: Methylobacterium cerastii Wellner et al. 2012
@ref: 17341
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium cerastii
full scientific name: Methylobacterium cerastii Wellner et al. 2012
strain designation: C15, C20oh1-15
type strain: yes
Morphology
cell morphology
- @ref: 30207
- gram stain: negative
- cell length: 1.6-5.4 µm
- cell width: 0.8-1.1 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 62192
- incubation period: 4 days
pigmentation
- @ref: 30207
- production: yes
Culture and growth conditions
culture medium
- @ref: 17341
- name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125)
- growth: yes
- link: https://mediadive.dsmz.de/medium/125
- composition: Name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125; with strain-specific modifications) Composition: Agar 12.0 g/l Methanol 5.0 g/l KNO3 1.0 g/l Na2HPO4 0.23 g/l MgSO4 x 7 H2O 0.2 g/l NaH2PO4 0.07 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l MoO3 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17341 | positive | growth | 28 | mesophilic |
30207 | positive | growth | 10-28 | |
30207 | positive | optimum | 20 | psychrophilic |
62192 | positive | growth | 25 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30207 | positive | growth | 04-06 | acidophile |
30207 | positive | optimum | 05-06 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
30207 | aerobe |
62192 | aerobe |
halophily
- @ref: 30207
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0.50 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30207 | 29987 | glutamate | + | carbon source |
30207 | 506227 | N-acetylglucosamine | + | carbon source |
30207 | 17148 | putrescine | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
30207 | catalase | + | 1.11.1.6 |
30207 | cytochrome oxidase | + | 1.9.3.1 |
30207 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|---|
17341 | leaf surface of Cerastium holosteoides | Cerastium holosteoides | Thüringen, exploratory Hainich-Dün | Germany | DEU | Europe | |
62192 | Leaf phyllosphere of Cerastium holosteoides | Thüringen | Germany | DEU | Europe | 2008 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Leaf (Phyllosphere) |
taxonmaps
- @ref: 69479
- File name: preview.99_17343.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_285;97_10713;98_13138;99_17343&stattab=map
- Last taxonomy: Methylobacterium
- 16S sequence: FR733885
- Sequence Identity:
- Total samples: 3957
- soil counts: 698
- aquatic counts: 353
- animal counts: 1785
- plant counts: 1121
Safety information
risk assessment
- @ref: 17341
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17341
- description: Methylobacterium cerastii partial 16S rRNA gene, isolate C15
- accession: FR733885
- length: 1353
- database: ena
- NCBI tax ID: 932741
Genome sequences
- @ref: 66792
- description: Methylobacterium cerastii DSM 23679
- accession: GCA_022179125
- assembly level: contig
- database: ncbi
- NCBI tax ID: 932741
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 79.169 | yes |
gram-positive | no | 97.713 | no |
anaerobic | no | 97.87 | yes |
aerobic | yes | 91.449 | yes |
halophile | no | 90.693 | yes |
spore-forming | no | 94.397 | no |
motile | yes | 86.285 | yes |
glucose-ferment | no | 89.368 | no |
thermophile | no | 99.061 | no |
glucose-util | yes | 89.205 | no |
External links
@ref: 17341
culture collection no.: DSM 23679, CCM 7788, CCUG 60040
straininfo link
- @ref: 76650
- straininfo: 379629
literature
- topic: Phylogeny
- Pubmed-ID: 21669927
- title: Methylobacterium cerastii sp. nov., isolated from the leaf surface of Cerastium holosteoides.
- authors: Wellner S, Lodders N, Kampfer P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.030767-0
- year: 2011
- mesh: Bacterial Typing Techniques, Caryophyllaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genotype, Germany, Methylobacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
17341 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23679) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23679 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30207 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26552 | 28776041 | |
62192 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 60040) | https://www.ccug.se/strain?id=60040 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76650 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID379629.1 | StrainInfo: A central database for resolving microbial strain identifiers |