Strain identifier

BacDive ID: 7197

Type strain: Yes

Species: Methylobacterium cerastii

Strain Designation: C15, C20oh1-15

Strain history: <- P. Kämpfer, Justus-Liebig-Universität; C20oh1-15 <- S.A. Wellner

NCBI tax ID(s): 932741 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17341

BacDive-ID: 7197

DSM-Number: 23679

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Methylobacterium cerastii C15 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from leaf surface of Cerastium holosteoides.

NCBI tax id

  • NCBI tax id: 932741
  • Matching level: species

strain history

  • @ref: 17341
  • history: <- P. Kämpfer, Justus-Liebig-Universität; C20oh1-15 <- S.A. Wellner

doi: 10.13145/bacdive7197.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylobacteriaceae
  • genus: Methylobacterium
  • species: Methylobacterium cerastii
  • full scientific name: Methylobacterium cerastii Wellner et al. 2012

@ref: 17341

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylobacteriaceae

genus: Methylobacterium

species: Methylobacterium cerastii

full scientific name: Methylobacterium cerastii Wellner et al. 2012

strain designation: C15, C20oh1-15

type strain: yes

Morphology

cell morphology

  • @ref: 30207
  • gram stain: negative
  • cell length: 1.6-5.4 µm
  • cell width: 0.8-1.1 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 62192
  • incubation period: 4 days

pigmentation

  • @ref: 30207
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 17341
  • name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/125
  • composition: Name: METHYLOBACTERIUM MEDIUM (DSMZ Medium 125; with strain-specific modifications) Composition: Agar 12.0 g/l Methanol 5.0 g/l KNO3 1.0 g/l Na2HPO4 0.23 g/l MgSO4 x 7 H2O 0.2 g/l NaH2PO4 0.07 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l MoO3 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17341positivegrowth28mesophilic
30207positivegrowth10-28
30207positiveoptimum20psychrophilic
62192positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
30207positivegrowth04-06acidophile
30207positiveoptimum05-06

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30207aerobe
62192aerobe

halophily

  • @ref: 30207
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.50 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3020729987glutamate+carbon source
30207506227N-acetylglucosamine+carbon source
3020717148putrescine+carbon source

enzymes

@refvalueactivityec
30207catalase+1.11.1.6
30207cytochrome oxidase+1.9.3.1
30207urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
17341leaf surface of Cerastium holosteoidesCerastium holosteoidesThüringen, exploratory Hainich-DünGermanyDEUEurope
62192Leaf phyllosphere of Cerastium holosteoidesThüringenGermanyDEUEurope2008

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_17343.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_285;97_10713;98_13138;99_17343&stattab=map
  • Last taxonomy: Methylobacterium
  • 16S sequence: FR733885
  • Sequence Identity:
  • Total samples: 3957
  • soil counts: 698
  • aquatic counts: 353
  • animal counts: 1785
  • plant counts: 1121

Safety information

risk assessment

  • @ref: 17341
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17341
  • description: Methylobacterium cerastii partial 16S rRNA gene, isolate C15
  • accession: FR733885
  • length: 1353
  • database: ena
  • NCBI tax ID: 932741

Genome sequences

  • @ref: 66792
  • description: Methylobacterium cerastii DSM 23679
  • accession: GCA_022179125
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 932741

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno79.169yes
gram-positiveno97.713no
anaerobicno97.87yes
aerobicyes91.449yes
halophileno90.693yes
spore-formingno94.397no
motileyes86.285yes
glucose-fermentno89.368no
thermophileno99.061no
glucose-utilyes89.205no

External links

@ref: 17341

culture collection no.: DSM 23679, CCM 7788, CCUG 60040

straininfo link

  • @ref: 76650
  • straininfo: 379629

literature

  • topic: Phylogeny
  • Pubmed-ID: 21669927
  • title: Methylobacterium cerastii sp. nov., isolated from the leaf surface of Cerastium holosteoides.
  • authors: Wellner S, Lodders N, Kampfer P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.030767-0
  • year: 2011
  • mesh: Bacterial Typing Techniques, Caryophyllaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genotype, Germany, Methylobacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17341Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23679)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23679
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30207Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2655228776041
62192Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 60040)https://www.ccug.se/strain?id=60040
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76650Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID379629.1StrainInfo: A central database for resolving microbial strain identifiers