Strain identifier
BacDive ID: 7195
Type strain: 
Species: Methylobacterium bullatum
Strain Designation: F3.2
Strain history: <- H. Hoffmann, Univ. Kassel, Germany; F3.2 <- M. Hornschuh
NCBI tax ID(s): 570505 (species)
version 8.1 (current version)
General
@ref: 16023
BacDive-ID: 7195
DSM-Number: 21893
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile
description: Methylobacterium bullatum F3.2 is an aerobe, mesophilic, motile bacterium that was isolated from cord moss Funaria hygrometrica.
NCBI tax id
- NCBI tax id: 570505
- Matching level: species
strain history
- @ref: 16023
- history: <- H. Hoffmann, Univ. Kassel, Germany; F3.2 <- M. Hornschuh
doi: 10.13145/bacdive7195.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium bullatum
- full scientific name: Methylobacterium bullatum Hoppe et al. 2012
@ref: 16023
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium bullatum
full scientific name: Methylobacterium bullatum Hoppe et al. 2012
strain designation: F3.2
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 96.99 | |
| 69480 | 99.995 | negative |
colony morphology
- @ref: 62819
- incubation period: 2 days
Culture and growth conditions
culture medium
- @ref: 16023
- name: MODIFIED METHYLOBACTERIUM MEDIUM (DSMZ Medium 1324)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1324
- composition: Name: MODIFIED METHYLOBACTERIUM MEDIUM (DSMZ Medium 1324) Composition: Agar 15.0 g/l Methanol 5.0 g/l MgSO4 x 6 H2O 1.0 g/l K2HPO4 0.7 g/l KH2PO4 0.54 g/l NH4Cl 0.5 g/l CaCl2 x 2 H2O 0.2 g/l FeSO4 x 7 H2O 0.004 g/l H3BO3 0.003 g/l CoCl2 x 6 H2O 0.002 g/l ZnSO4 x 7 H2O 0.001 g/l Na2MoO4 x 2 H2O 0.0006 g/l MnCl2 x 4 H2O 0.0003 g/l NiCl2 x 6 H2O 0.0002 g/l CuCl2 x 2 H2O 0.0001 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16023 | positive | growth | 28 | mesophilic |
| 62819 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 62819
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.987 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|---|---|
| 16023 | cord moss Funaria hygrometrica | Funaria hygrometrica | Kassel, Bergpark Wilhelmshöhe | Germany | DEU | Europe | |
| 62819 | Plant,phylloid of Funaria hygrometrica | Hesse,Kassel | Germany | DEU | Europe | 2002 |
isolation source categories
- Cat1: #Host
- Cat2: #Plants
- Cat3: #Moss
taxonmaps
- @ref: 69479
- File name: preview.99_790.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_285;97_548;98_631;99_790&stattab=map
- Last taxonomy: Methylobacterium bullatum
- 16S sequence: FJ268657
- Sequence Identity:
- Total samples: 24347
- soil counts: 5760
- aquatic counts: 3552
- animal counts: 8689
- plant counts: 6346
Sequence information
16S sequences
- @ref: 16023
- description: Methylobacterium bullatum strain F3.2 16S ribosomal RNA gene, partial sequence
- accession: FJ268657
- length: 1448
- database: ena
- NCBI tax ID: 570505
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methylobacterium bullatum LMG 24788 | GCA_014845095 | contig | ncbi | 570505 |
| 66792 | Methylobacterium bullatum strain LMG 24788 | 570505.5 | wgs | patric | 570505 |
| 66792 | Methylobacterium bullatum DSM 21893 | GCA_022179105 | contig | ncbi | 570505 |
GC content
- @ref: 16023
- GC-content: 67.1
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| gram-positive | no | 96.789 | no |
| anaerobic | no | 98.348 | yes |
| halophile | no | 93.448 | no |
| spore-forming | no | 93.676 | no |
| glucose-util | yes | 88.817 | no |
| thermophile | no | 99.373 | no |
| flagellated | no | 73.208 | no |
| aerobic | yes | 92.867 | no |
| motile | yes | 86.868 | no |
| glucose-ferment | no | 87.615 | no |
External links
@ref: 16023
culture collection no.: DSM 21893, LMG 24788, CCUG 62366
straininfo link
- @ref: 76648
- straininfo: 359370
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 21612883 | Methylobacterium bullatum sp. nov., a methylotrophic bacterium isolated from Funaria hygrometrica. | Hoppe T, Peters K, Schmidt F | Syst Appl Microbiol | 10.1016/j.syapm.2010.12.005 | 2011 | Alcohol Oxidoreductases/genetics, Bacteria, Aerobic/classification/genetics/isolation & purification/physiology/ultrastructure, Bacterial Typing Techniques, Base Composition, Biofilms, Bryopsida/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Genes, Bacterial, Hydrogen-Ion Concentration, Methylobacterium/*classification/genetics/*isolation & purification/physiology/ultrastructure, Microscopy, Electron, Phenotype, Phylogeny, Pigmentation, Ribosomes/genetics, Salt Tolerance | Enzymology |
| Phylogeny | 23291886 | Methylobacterium trifolii sp. nov. and Methylobacterium thuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces. | Wellner S, Lodders N, Glaeser SP, Kampfer P | Int J Syst Evol Microbiol | 10.1099/ijs.0.047787-0 | 2013 | Bacterial Typing Techniques, Caryophyllaceae/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Germany, Methanol/*metabolism, Methylobacterium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, *Pigmentation, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Trifolium/*microbiology | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 16023 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21893) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21893 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 62819 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 62366) | https://www.ccug.se/strain?id=62366 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 76648 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID359370.1 | StrainInfo: A central database for resolving microbial strain identifiers |