Strain identifier
BacDive ID: 7194
Type strain: 
Species: Methylobacterium longum
Strain Designation: 440
Strain history: <- C. Knief, ETHZ, Switzerland; strain 440 <- Laboratory of Plant Microbe Interactions (LIPM), Toulouse, France
NCBI tax ID(s): 767694 (species)
version 8.1 (current version)
General
@ref: 17442
BacDive-ID: 7194
DSM-Number: 23933
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Methylobacterium longum 440 is a mesophilic bacterium that was isolated from phyllosphere of Arabidopsis thaliana.
NCBI tax id
- NCBI tax id: 767694
- Matching level: species
strain history
- @ref: 17442
- history: <- C. Knief, ETHZ, Switzerland; strain 440 <- Laboratory of Plant Microbe Interactions (LIPM), Toulouse, France
doi: 10.13145/bacdive7194.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium longum
- full scientific name: Methylobacterium longum Knief et al. 2012
@ref: 17442
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium longum
full scientific name: Methylobacterium longum Knief et al. 2012
strain designation: 440
type strain: yes
Culture and growth conditions
culture medium
- @ref: 17442
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
- @ref: 17442
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 17442
- sample type: phyllosphere of Arabidopsis thaliana
- host species: Arabidopsis thaliana
- country: Spain
- origin.country: ESP
- continent: Europe
- latitude: 41.2147
- longitude: -4.5444
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Leaf (Phyllosphere) |
taxonmaps
- @ref: 69479
- File name: preview.99_6957.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_285;97_315;98_350;99_6957&stattab=map
- Last taxonomy: Methylobacterium
- 16S sequence: FN868949
- Sequence Identity:
- Total samples: 3690
- soil counts: 439
- aquatic counts: 1020
- animal counts: 1696
- plant counts: 535
Safety information
risk assessment
- @ref: 17442
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17442
- description: Methylobacterium longum partial 16S rRNA gene, type strain 440
- accession: FN868949
- length: 1404
- database: ena
- NCBI tax ID: 767694
Genome sequences
- @ref: 66792
- description: Methylobacterium longum DSM 23933
- accession: GCA_022179385
- assembly level: contig
- database: ncbi
- NCBI tax ID: 767694
GC content
- @ref: 17442
- GC-content: 68.6
- method: fluorimetric
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 77.637 | no |
| gram-positive | no | 97.64 | no |
| anaerobic | no | 98.523 | no |
| aerobic | yes | 91.29 | no |
| halophile | no | 91.184 | no |
| spore-forming | no | 94.051 | no |
| motile | yes | 88.308 | no |
| glucose-ferment | no | 89.082 | no |
| thermophile | no | 99.146 | no |
| glucose-util | yes | 87.031 | no |
External links
@ref: 17442
culture collection no.: DSM 23933, CECT 7806
straininfo link
- @ref: 76647
- straininfo: 379814
literature
- topic: Phylogeny
- Pubmed-ID: 21986935
- title: Characterization of Methylobacterium strains isolated from the phyllosphere and description of Methylobacterium longum sp. nov.
- authors: Knief C, Dengler V, Bodelier PL, Vorholt JA
- journal: Antonie Van Leeuwenhoek
- DOI: 10.1007/s10482-011-9650-6
- year: 2011
- mesh: Bacterial Typing Techniques, Base Composition, Carbon/metabolism, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Methanol/metabolism, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/metabolism, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 17442 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23933) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23933 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 76647 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID379814.1 | StrainInfo: A central database for resolving microbial strain identifiers |