Strain identifier
BacDive ID: 7180
Type strain:
Species: Methylobacterium adhaesivum
Strain Designation: AR27
Strain history: <- A. Ventosa <- V. Gallego; AR27
NCBI tax ID(s): 333297 (species)
General
@ref: 6804
BacDive-ID: 7180
DSM-Number: 17169
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Methylobacterium adhaesivum AR27 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from drinking water.
NCBI tax id
- NCBI tax id: 333297
- Matching level: species
strain history
- @ref: 6804
- history: <- A. Ventosa <- V. Gallego; AR27
doi: 10.13145/bacdive7180.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylobacterium
- species: Methylobacterium adhaesivum
- full scientific name: Methylobacterium adhaesivum Gallego et al. 2006
@ref: 6804
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylobacterium
species: Methylobacterium adhaesivum
full scientific name: Methylobacterium adhaesivum Gallego et al. 2006
strain designation: AR27
type strain: yes
Morphology
cell morphology
- @ref: 31650
- gram stain: negative
- cell length: 1.5-5 µm
- cell width: 1-1.2 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 31650
- production: yes
Culture and growth conditions
culture medium
- @ref: 6804
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6804 | positive | growth | 28 | mesophilic |
31650 | positive | growth | 15-35 | |
31650 | positive | optimum | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31650 | positive | growth | 05-09 | alkaliphile |
31650 | positive | optimum | 6.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 31650
- oxygen tolerance: aerobe
spore formation
- @ref: 31650
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31650 | NaCl | positive | growth | 0-1 % |
31650 | NaCl | positive | optimum | 0-1 % |
observation
- @ref: 31650
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31650 | 30089 | acetate | + | carbon source |
31650 | 28757 | fructose | + | carbon source |
31650 | 29987 | glutamate | + | carbon source |
31650 | 17754 | glycerol | + | carbon source |
31650 | 24996 | lactate | + | carbon source |
31650 | 25115 | malate | + | carbon source |
31650 | 51850 | methyl pyruvate | + | carbon source |
31650 | 17272 | propionate | + | carbon source |
31650 | 15361 | pyruvate | + | carbon source |
31650 | 30031 | succinate | + | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
31650 | catalase | + | 1.11.1.6 |
31650 | cytochrome oxidase | + | 1.9.3.1 |
31650 | urease | + | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 6804
- sample type: drinking water
- geographic location: Seville
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Freshwater
taxonmaps
- @ref: 69479
- File name: preview.99_2004.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_372;97_420;98_479;99_2004&stattab=map
- Last taxonomy: Methylobacterium
- 16S sequence: AM040156
- Sequence Identity:
- Total samples: 14971
- soil counts: 2415
- aquatic counts: 1965
- animal counts: 7622
- plant counts: 2969
Safety information
risk assessment
- @ref: 6804
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Methylobacterium adhaesivum gene for 16S rRNA, partial sequence, strain: DSM 17169 | AB302928 | 1435 | ena | 333297 |
6804 | Methylobacterium adhaesivum partial 16S rRNA gene, type strain AR27T | AM040156 | 1391 | ena | 333297 |
Genome sequences
- @ref: 66792
- description: Methylobacterium adhaesivum DSM 17169
- accession: GCA_022179065
- assembly level: contig
- database: ncbi
- NCBI tax ID: 333297
GC content
@ref | GC-content | method |
---|---|---|
6804 | 63.6 | thermal denaturation, midpoint method (Tm) |
31650 | 63.6 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 76.98 | no |
gram-positive | no | 97.679 | no |
anaerobic | no | 97.328 | yes |
aerobic | yes | 90.916 | yes |
halophile | no | 89.637 | yes |
spore-forming | no | 94.087 | yes |
motile | yes | 85.689 | yes |
glucose-ferment | no | 90.952 | no |
thermophile | no | 99.208 | yes |
glucose-util | yes | 83.967 | no |
External links
@ref: 6804
culture collection no.: DSM 17169, CCM 7305, CECT 7069
straininfo link
- @ref: 76633
- straininfo: 289979
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 16449436 | Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water. | Gallego V, Garcia MT, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijs.0.63966-0 | 2006 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water Supply | Genetics |
Phylogeny | 18175690 | Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea. | Weon HY, Kim BY, Joa JH, Son JA, Song MH, Kwon SW, Go SJ, Yoon SH | Int J Syst Evol Microbiol | 10.1099/ijs.0.65047-0 | 2008 | *Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
6804 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17169) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17169 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
31650 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 27932 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
76633 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID289979.1 | StrainInfo: A central database for resolving microbial strain identifiers |