Strain identifier

BacDive ID: 7180

Type strain: Yes

Species: Methylobacterium adhaesivum

Strain Designation: AR27

Strain history: <- A. Ventosa <- V. Gallego; AR27

NCBI tax ID(s): 333297 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6804

BacDive-ID: 7180

DSM-Number: 17169

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Methylobacterium adhaesivum AR27 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from drinking water.

NCBI tax id

  • NCBI tax id: 333297
  • Matching level: species

strain history

  • @ref: 6804
  • history: <- A. Ventosa <- V. Gallego; AR27

doi: 10.13145/bacdive7180.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylobacteriaceae
  • genus: Methylobacterium
  • species: Methylobacterium adhaesivum
  • full scientific name: Methylobacterium adhaesivum Gallego et al. 2006

@ref: 6804

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylobacteriaceae

genus: Methylobacterium

species: Methylobacterium adhaesivum

full scientific name: Methylobacterium adhaesivum Gallego et al. 2006

strain designation: AR27

type strain: yes

Morphology

cell morphology

  • @ref: 31650
  • gram stain: negative
  • cell length: 1.5-5 µm
  • cell width: 1-1.2 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 31650
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 6804
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6804positivegrowth28mesophilic
31650positivegrowth15-35
31650positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
31650positivegrowth05-09alkaliphile
31650positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 31650
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31650
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31650NaClpositivegrowth0-1 %
31650NaClpositiveoptimum0-1 %

observation

  • @ref: 31650
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3165030089acetate+carbon source
3165028757fructose+carbon source
3165029987glutamate+carbon source
3165017754glycerol+carbon source
3165024996lactate+carbon source
3165025115malate+carbon source
3165051850methyl pyruvate+carbon source
3165017272propionate+carbon source
3165015361pyruvate+carbon source
3165030031succinate+carbon source

enzymes

@refvalueactivityec
31650catalase+1.11.1.6
31650cytochrome oxidase+1.9.3.1
31650urease+3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 6804
  • sample type: drinking water
  • geographic location: Seville
  • country: Spain
  • origin.country: ESP
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_2004.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_372;97_420;98_479;99_2004&stattab=map
  • Last taxonomy: Methylobacterium
  • 16S sequence: AM040156
  • Sequence Identity:
  • Total samples: 14971
  • soil counts: 2415
  • aquatic counts: 1965
  • animal counts: 7622
  • plant counts: 2969

Safety information

risk assessment

  • @ref: 6804
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methylobacterium adhaesivum gene for 16S rRNA, partial sequence, strain: DSM 17169AB3029281435ena333297
6804Methylobacterium adhaesivum partial 16S rRNA gene, type strain AR27TAM0401561391ena333297

Genome sequences

  • @ref: 66792
  • description: Methylobacterium adhaesivum DSM 17169
  • accession: GCA_022179065
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 333297

GC content

@refGC-contentmethod
680463.6thermal denaturation, midpoint method (Tm)
3165063.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno76.98no
gram-positiveno97.679no
anaerobicno97.328yes
aerobicyes90.916yes
halophileno89.637yes
spore-formingno94.087yes
motileyes85.689yes
glucose-fermentno90.952no
thermophileno99.208yes
glucose-utilyes83.967no

External links

@ref: 6804

culture collection no.: DSM 17169, CCM 7305, CECT 7069

straininfo link

  • @ref: 76633
  • straininfo: 289979

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16449436Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water.Gallego V, Garcia MT, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.63966-02006Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Genes, rRNA, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water SupplyGenetics
Phylogeny18175690Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea.Weon HY, Kim BY, Joa JH, Son JA, Song MH, Kwon SW, Go SJ, Yoon SHInt J Syst Evol Microbiol10.1099/ijs.0.65047-02008*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6804Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17169)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17169
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31650Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127932
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76633Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID289979.1StrainInfo: A central database for resolving microbial strain identifiers