Strain identifier
BacDive ID: 7170
Type strain:
Species: Methylorubrum zatmanii
Strain Designation: 135
Strain history: CIP <- 1993, NCIMB <- R.I. Mateles, Jerusalem, Israel, Pseudomonas 135 <- J.S. Rock
NCBI tax ID(s): 29429 (species)
General
@ref: 2285
BacDive-ID: 7170
DSM-Number: 5688
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile
description: Methylorubrum zatmanii 135 is a mesophilic, Gram-negative, motile bacterium that was isolated from fermentor.
NCBI tax id
- NCBI tax id: 29429
- Matching level: species
strain history
@ref | history |
---|---|
2285 | <- NCIMB <- R.I. Mateles, 135 <- J.S. Rock |
67770 | NCIMB 12243 <-- R. I. Mateles 135 <-- J. S. Rock. |
124022 | CIP <- 1993, NCIMB <- R.I. Mateles, Jerusalem, Israel, Pseudomonas 135 <- J.S. Rock |
doi: 10.13145/bacdive7170.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylobacteriaceae
- genus: Methylorubrum
- species: Methylorubrum zatmanii
- full scientific name: Methylorubrum zatmanii (Green et al. 1988) Green and Ardley 2018
synonyms
- @ref: 20215
- synonym: Methylobacterium zatmanii
@ref: 2285
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylobacteriaceae
genus: Methylorubrum
species: Methylorubrum zatmanii
full scientific name: Methylorubrum zatmanii (Green et al. 1988) Green and Ardley 2018
strain designation: 135
type strain: yes
Morphology
cell morphology
- @ref: 124022
- gram stain: negative
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2285 | COLBY AND ZATHMAN MEDIUM (DSMZ Medium 606) | yes | https://mediadive.dsmz.de/medium/606 | Name: COLBY AND ZATHMAN MEDIUM (DSMZ Medium 606) Composition: Agar 15.0 g/l Methanol 1.584 g/l K2HPO4 1.2 g/l KH2PO4 0.62 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l NaCl 0.1 g/l CaCl2 x 6 H2O 0.05 g/l FeCl3 x 6 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l Na2MoO4 x 2 H2O 1e-05 g/l H3BO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l CoCl2 x 6 H2O 5e-06 g/l Distilled water |
40783 | MEDIUM 53 - for Marinomonas and Methylobacterium (except Methylobacterium organophilum) | yes | Distilled water make up to (1000.000 ml);Methanol (5.000 ml);Brain heartinfusion (37.000 g) | |
124022 | CIP Medium 53 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=53 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2285 | positive | growth | 30 | mesophilic |
40783 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
124022 | positive | growth | 30 | mesophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
124022 | 17632 | nitrate | - | reduction |
124022 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 124022
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
124022 | 15688 | acetoin | - | |
124022 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
124022 | oxidase | + | |
124022 | beta-galactosidase | - | 3.2.1.23 |
124022 | alcohol dehydrogenase | - | 1.1.1.1 |
124022 | gelatinase | - | |
124022 | DNase | - | |
124022 | caseinase | - | 3.4.21.50 |
124022 | catalase | + | 1.11.1.6 |
124022 | lecithinase | - | |
124022 | lysine decarboxylase | - | 4.1.1.18 |
124022 | ornithine decarboxylase | - | 4.1.1.17 |
124022 | urease | + | 3.5.1.5 |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
124022 | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | + | - | + | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
2285 | fermentor | ||||
67770 | Fermentor operating with formaldehyde as a sole carbon source | ||||
124022 | Other, Fermentor | Jerusalem | Israel | ISR | Asia |
isolation source categories
- Cat1: #Engineered
- Cat2: #Bioreactor
taxonmaps
- @ref: 69479
- File name: preview.99_253.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15368;96_177;97_192;98_215;99_253&stattab=map
- Last taxonomy: Methylorubrum
- 16S sequence: AB175647
- Sequence Identity:
- Total samples: 110728
- soil counts: 23280
- aquatic counts: 23711
- animal counts: 47578
- plant counts: 16159
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
2285 | 1 | Risk group (German classification) |
124022 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Methylobacterium zatmanii gene for 16S ribosomal RNA, partial sequence, strain:DSM 5688 | AB175647 | 1435 | ena | 29429 |
20218 | Methylobacterium zatmanii (NCIMB 12243) 16S ribosomal RNA | L20804 | 1316 | ena | 29429 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methylorubrum zatmanii LMG 6087 | GCA_014845115 | contig | ncbi | 29429 |
66792 | Methylorubrum zatmanii strain LMG 6087 | 29429.5 | wgs | patric | 29429 |
GC content
- @ref: 67770
- GC-content: 70.3
- method: Buoyant density centrifugation (BD)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
gram-positive | no | 97.862 | no |
anaerobic | no | 97.319 | no |
halophile | no | 92.656 | no |
spore-forming | no | 92.529 | no |
glucose-util | yes | 87.366 | no |
motile | yes | 87.554 | no |
flagellated | no | 73.023 | no |
aerobic | yes | 89.24 | no |
thermophile | no | 99.122 | yes |
glucose-ferment | no | 89.049 | no |
External links
@ref: 2285
culture collection no.: DSM 5688, ATCC 43883, NCIMB 12243, CCUG 36916, CIP 103774, LMG 6087, IFO 15845, VKM B-2161, JCM 10892, CCM 4464, NBRC 15845
straininfo link
- @ref: 76623
- straininfo: 13721
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24297603 | Methylobacterium pseudosasae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the bamboo phyllosphere. | Madhaiyan M, Poonguzhali S | Antonie Van Leeuwenhoek | 10.1007/s10482-013-0085-0 | 2013 | Acyl-Butyrolactones/analysis, Alcohol Oxidoreductases/genetics, Bambusa/*microbiology, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Methanol/*metabolism, Methylobacterium/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Enzymology |
Phylogeny | 30024371 | Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov. | Green PN, Ardley JK | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002856 | 2018 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Methylobacterium/*classification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2285 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5688) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5688 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40783 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15608 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76623 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13721.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
124022 | Curators of the CIP | Collection of Institut Pasteur (CIP 103774) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103774 |